BMRBj - BMREntryMaster

Found 1 Document (0.551 seconds)

BMRB: 5250

3RIO

1H, 13C and 15N resonance assignment of the RNA-binding domain dimer form Bacillus subtilis transcriptional antiterminator GlcT

Authors

Stoldt, M., Langbein, I., Stuelke, J., Gorlach, M.

Assembly

GlcT-RBD dimer

Entity

1. GlcT-RBD dimer (polymer, Thiol state: not present), 74 monomers, 8378.388 × 2 Da Detail

MRGSHHHHHH GSVDVNGSFT VKKVLNNNVL IASHHKYSEV VLIGKGIGFG KKQDDVIEDK GYDKMFILKD EKEQ

Total weight

16756.775 Da

Max. entity weight

8378.388 Da

Source organism

Bacillus subtilis

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.6 %, Completeness: 88.9 %, Completeness (bb): 90.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.9 % (778 of 875)	89.7 % (409 of 456)	87.9 % (298 of 339)	88.7 % (71 of 80)
Backbone	90.5 % (402 of 444)	91.0 % (142 of 156)	91.1 % (195 of 214)	87.8 % (65 of 74)
Sidechain	87.9 % (437 of 497)	89.0 % (267 of 300)	85.9 % (164 of 191)	100.0 % (6 of 6)
Aromatic	43.6 % (34 of 78)	43.6 % (17 of 39)	43.6 % (17 of 39)
Methyl	100.0 % (72 of 72)	100.0 % (36 of 36)	100.0 % (36 of 36)

1. RNA-binding domain of transcriptional antiterminator GlcT

MRGSHHHHHH GSVDVNGSFT VKKVLNNNVL IASHHKYSEV VLIGKGIGFG KKQDDVIEDK GYDKMFILKD EKEQ

Show sample condition

Sample

Temperature 298 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	RNA-binding domain of transcriptional antiterminator GlcT	[U-13C; U-15N]		2.0 ~ 3.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.55 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.55 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.25 ppm, Outliers: 1 Detail

Release date

2002-09-25

Citation

1H, 13C and 15N resonance assignment of the RNA-binding domain dimer form Bacillus subtilis transcriptional antiterminator GlcT
Stoldt, M., Langbein, I., Stuelke, J., Gorlach, M.

Related entities 1. GlcT-RBD dimer, : 1 : 1 entities Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5250_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5250_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	854		94.6 (chain: 1, length: 74)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 74, Sequence coverage: 94.6%
- Total number of assignments
  - ¹³C chemical shifts: 298
  - ¹H chemical shifts: 485
  - ¹⁵N chemical shifts: 71
- Completeness of assignments
  - Entity_assemble_ID: 1
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

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Found 1 Document (0.551 seconds)

BMRB: 5250

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. GlcT-RBD dimer, : 1 : 1 entities Detail

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	339	298	87.9
¹H chemical shifts	456	409	89.7
¹⁵N chemical shifts	81	71	87.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	148	134	90.5
¹H chemical shifts	156	142	91.0
¹⁵N chemical shifts	74	65	87.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	191	164	85.9
¹H chemical shifts	300	267	89.0
¹⁵N chemical shifts	7	6	85.7