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Structural Differences in the NOE-derived Structure of G-T Mismatched DNA relative to Normal DNA are Correlated with Differences in (13)C Relaxation-based Internal Dynamics
Authors
Isaacs, R.J., Rayens, W.S., Spielmann, H.P.
Assembly
5'-D(*CP*CP*AP*CP*GP*CP*GP*TP*GP*G)-3'
Entity
1. 5'-D(*CP*CP*AP*CP*GP*CP*GP*TP*GP*G)-3' (polymer, Thiol state: not present), 10 monomers, 3108.964 × 2 Da Detail

CCACGCGTGG


Total weight
6217.928 Da
Max. entity weight
3108.964 Da
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 80.8 %, Completeness (bb): 91.4 % Detail

Polymer type: polydeoxyribonucleotide

Total1H
All80.8 % (80 of 99)80.8 % (80 of 99)
Suger, PO491.4 % (64 of 70)91.4 % (64 of 70)
Nucleobase55.2 % (16 of 29)55.2 % (16 of 29)
Aromatic75.0 % (15 of 20)75.0 % (15 of 20)
Methyl100.0 % (1 of 1)100.0 % (1 of 1)

1. 5'-D(*CP*CP*AP*CP*GP*CP*GP*TP*GP*G)-3'

CCACGCGTGG

Sample

Pressure 1 atm, Temperature 298 K, pH 7.0


#NameIsotope labelingTypeConcentration
15'-D(*CP*CP*AP*CP*GP*CP*GP*TP*GP*G)-3'8 mL
2NaCl200 mM
3NaPO420 mM
4NaN310 mM
5EDTA0.1 mM
6D2O99.96 %

Release date
2002-09-09
Citation
Structural differences in the NOE-derived structure of G-T mismatched DNA relative to normal DNA are correlated with differences in (13)C relaxation-based internal dynamics
Isaacs, R.J., Rayens, W.S., Spielmann, H.P.
J. Mol. Biol. (2002), 319, 191-207, PubMed 12051946 , DOI 10.1016/S0022-2836(02)00265-6 ,
Entries sharing articles BMRB: 1 entries Detail
  BMRB: 5253 released on 2002-09-09
    Title Structural Differences in the NOE-derived Structure of G-T Mismatched DNA relative to Normal DNA are Correlated with Differences in (13)C Relaxation-based Internal Dynamics
Experiments performed 1 experiments Detail
Keywords dynamics, G-T mismatch, helical parameter, order parameter, sequence dependent dynamics, sequence dependent structure, spin relaxation