BMRB: 5252

Structural Differences in the NOE-derived Structure of G-T Mismatched DNA relative to Normal DNA are Correlated with Differences in (13)C Relaxation-based Internal Dynamics

Authors

Isaacs, R.J., Rayens, W.S., Spielmann, H.P.

Assembly

5'-D(*CP*CP*AP*CP*GP*CP*GP*TP*GP*G)-3'

Entity

1. 5'-D(*CP*CP*AP*CP*GP*CP*GP*TP*GP*G)-3' (polymer, Thiol state: not present), 10 monomers, 3108.964 × 2 Da Detail

CCACGCGTGG

Total weight

6217.928 Da

Max. entity weight

3108.964 Da

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 80.8 %, Completeness (bb): 91.4 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	80.8 % (80 of 99)	80.8 % (80 of 99)
Suger, PO4	91.4 % (64 of 70)	91.4 % (64 of 70)
Nucleobase	55.2 % (16 of 29)	55.2 % (16 of 29)
Aromatic	75.0 % (15 of 20)	75.0 % (15 of 20)
Methyl	100.0 % (1 of 1)	100.0 % (1 of 1)

1. 5'-D(*CP*CP*AP*CP*GP*CP*GP*TP*GP*G)-3'

CCACGCGTGG

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	5'-D(*CP*CP*AP*CP*GP*CP*GP*TP*GP*G)-3'			8 mL
2	NaCl			200 mM
3	NaPO4			20 mM
4	NaN3			10 mM
5	EDTA			0.1 mM
6	D2O			99.96 %

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Release date

2002-09-09

Citation

Structural differences in the NOE-derived structure of G-T mismatched DNA relative to normal DNA are correlated with differences in (13)C relaxation-based internal dynamics
Isaacs, R.J., Rayens, W.S., Spielmann, H.P.
J. Mol. Biol. (2002), 319, 191-207, PubMed 12051946 , DOI 10.1016/S0022-2836(02)00265-6 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Experiments performed 1 experiments Detail