BMRBj - BMREntryMaster

Found 1 Document (1.563 seconds)

BMRB: 5266

2L6F

1H, 15N and 13C assignments of the focal adhesion targeting domain of focal adhesion kinase (FAT)

Authors

Liu, G., Guibao, C.D., Zheng, J.

Assembly

FAT domain of Focal adhesion kinase (914-1053)

Entity

1. FAT domain of Focal adhesion kinase (914-1053) (polymer, Thiol state: not present), 139 monomers, 15525.94 Da Detail

MANLDRSNDK VYENVTGLVK AVIEMSSKIQ PAPPEEYVPM VKEVGLALRT LLATVDESLP VLPASTHREI EMAQKLLNSD LAELINKMKL AQQYVMTSLQ QEYKKQMLTA AHALAVDAKN LLDVIDQARL KMISQSRPH

Formula weight

15525.94 Da

Source organism

Gallus gallus

Exptl. method

NMR

Refine. method

distance geometry and torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.3 %, Completeness: 37.5 %, Completeness (bb): 67.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	37.5 % (613 of 1633)	23.2 % (198 of 852)	45.4 % (288 of 634)	86.4 % (127 of 147)
Backbone	67.3 % (552 of 820)	52.0 % (142 of 273)	68.4 % (284 of 415)	95.5 % (126 of 132)
Sidechain	20.5 % (195 of 950)	9.8 % (57 of 579)	38.5 % (137 of 356)	6.7 % (1 of 15)
Aromatic	9.1 % (4 of 44)	18.2 % (4 of 22)	0.0 % (0 of 22)
Methyl	13.7 % (26 of 190)	9.5 % (9 of 95)	17.9 % (17 of 95)

1. Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)

MANLDRSNDK VYENVTGLVK AVIEMSSKIQ PAPPEEYVPM VKEVGLALRT LLATVDESLP VLPASTHREI EMAQKLLNSD LAELINKMKL AQQYVMTSLQ QEYKKQMLTA AHALAVDAKN LLDVIDQARL KMISQSRPH

Show sample condition

Sample #1

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-15N]		1.0 mL

Sample #2

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-13C; U-15N]		2.0 mL

Protein Blocks Logo

Calculated from 25 models in PDB: 1KTM, Strand ID: A Detail

Release date

2003-01-05

Citation

1H, 15N and 13C assignments of the targeting (FAT) domain of focal adhesion kinase
Liu, G., Guibao, C.D., Zheng, J.
J. Biomol. NMR (2002), 23, 75-76, PubMed 12061723 , DOI: , Abstract

Related entities 1. FAT domain of Focal adhesion kinase (914-1053), : 1 : 3 : 3 : 42 entities Detail

Interaction partners 1. FAT domain of Focal adhesion kinase (914-1053), : 6 interactors Detail

More details for 6 interactors identified by 5 citations

Experiments performed 12 experiments Detail

1 1H-15N HAQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-15N]		1.0 mL

Sample #2

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-13C; U-15N]		2.0 mL

2 1H-15N NOESYHSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-15N]		1.0 mL

Sample #2

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-13C; U-15N]		2.0 mL

3 1H-15N TOCSYHSQC

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-15N]		1.0 mL

Sample #2

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-13C; U-15N]		2.0 mL

4 HNCA

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-15N]		1.0 mL

Sample #2

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-13C; U-15N]		2.0 mL

5 HNCACB

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-15N]		1.0 mL

Sample #2

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-13C; U-15N]		2.0 mL

6 CBCACONH

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-15N]		1.0 mL

Sample #2

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-13C; U-15N]		2.0 mL

7 HN(CO)CA

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-15N]		1.0 mL

Sample #2

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-13C; U-15N]		2.0 mL

8 3D C13-edit NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-15N]		1.0 mL

Sample #2

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-13C; U-15N]		2.0 mL

9 3D HCCHTOCSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-15N]		1.0 mL

Sample #2

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-13C; U-15N]		2.0 mL

10 3D HCCHCOSY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-15N]		1.0 mL

Sample #2

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-13C; U-15N]		2.0 mL

11 4D 15N-C13 NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-15N]		1.0 mL

Sample #2

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-13C; U-15N]		2.0 mL

12 4D C13-C13 NOESY

Instrument

Model Varian INOVA (600 MHz)

Sample #1

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-15N]		1.0 mL

Sample #2

Temperature 310 (±1) K, pH 6.2 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	Focal Adhesion Targeting domain of focal adhesion kinase (914-1053)	[U-13C; U-15N]		2.0 mL

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5266_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5266_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	520		99.3 (chain: 1, length: 139)
1	Chemical shift references	chemical_shift_reference	OK	5		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 139, Sequence coverage: 99.3%
- Total number of assignments
  - ¹³C chemical shifts: 270
  - ¹⁵N chemical shifts: 125
  - ¹H chemical shifts: 125
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	278	138	49.6
¹⁵N chemical shifts	132	124	93.9
¹H chemical shifts	273	125	45.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	103	15	14.6
¹H chemical shifts	103	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	634	270	42.6
¹⁵N chemical shifts	152	125	82.2
¹H chemical shifts	852	125	14.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	356	132	37.1
¹⁵N chemical shifts	20	1	5.0
¹H chemical shifts	579	0	0.0

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Found 1 Document (1.563 seconds)

BMRB: 5266

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Related entities 1. FAT domain of Focal adhesion kinase (914-1053), : 1 : 3 : 3 : 42 entities Detail

Interaction partners 1. FAT domain of Focal adhesion kinase (914-1053), : 6 interactors Detail

Experiments performed 12 experiments Detail

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Chemical shift validation 3 contents Detail