BMRBj - BMREntryMaster

Found 1 Document (1.612 seconds)

BMRB: 5286

1A06

Backbone 1H, 13C, and 15N Chemical shift assigments for [13C,15N]CaM:CaMKIp complex

Authors

Kranz, J.K., Lee, E.K., Nairn, A.C., Wand, A.JOSHUA.

Assembly

CaM:CaMKIp complex

Entity

1. calmodulin (polymer, Thiol state: not present), 148 monomers, 16706.21 Da Detail

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. CaM-binding sequence(299-320) from CaMKI (polymer, Thiol state: not present), 23 monomers, 2639.108 Da Detail

XKSKWKQAFN ATAVVRHMRK LQX

Total weight

19345.318 Da

Max. entity weight

16706.21 Da

Source organism

Gallus gallus , Rattus norvegicus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.5 %, Completeness: 40.2 %, Completeness (bb): 72.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	40.2 % (782 of 1944)	18.9 % (191 of 1013)	59.6 % (446 of 748)	79.2 % (145 of 183)
Backbone	72.6 % (733 of 1010)	44.7 % (155 of 347)	87.3 % (433 of 496)	86.8 % (145 of 167)
Sidechain	17.0 % (186 of 1092)	5.4 % (36 of 666)	36.6 % (150 of 410)	0.0 % (0 of 16)
Aromatic	0.0 % (0 of 126)	0.0 % (0 of 63)	0.0 % (0 of 62)	0.0 % (0 of 1)
Methyl	13.6 % (22 of 162)	8.6 % (7 of 81)	18.5 % (15 of 81)

1. calmodulin

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. CaM-binding sequence(299-320) from CaMKI

XKSKWKQAFN ATAVVRHMRK LQX

Show sample condition

Sample

Temperature 303 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	calmodulin	[U-99% 13C; U-99% 15N]		2 mM
2	CaM-binding sequence(299-320) from CaMKI			2.25 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.87 ppm, Outliers: 3 Detail

Release date

2002-06-12

Citation

A direct test of the reductionist approach to structural studies of calmodulin activity: relevance of peptide models of target proteins
Kranz, J.K., Lee, E.K., Nairn, A.C., Wand, A.JOSHUA.
J. Biol. Chem. (2002), 277, 16351-16354, PubMed 11904288 , DOI 10.1074/jbc.C200139200 , Abstract

Related entities 1. calmodulin, : 11 : 88 entities Detail

Related entities 2. CaM-binding sequence(299-320) from CaMKI, : 1 : 7 : 19 entities Detail

Experiments performed 5 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5286_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5286_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	718		99.3 (chain: 1, length: 148)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 148, Sequence coverage: 99.3%
- Total number of assignments
  - ¹³C chemical shifts: 430
  - ¹H chemical shifts: 144
  - ¹⁵N chemical shifts: 144
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	645	430	66.7
¹H chemical shifts	867	144	16.6
¹⁵N chemical shifts	164	144	87.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	296	294	99.3
¹H chemical shifts	305	144	47.2
¹⁵N chemical shifts	146	144	98.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	80	10	12.5
¹H chemical shifts	80	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	349	136	39.0
¹H chemical shifts	562	0	0.0
¹⁵N chemical shifts	18	0	0.0

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Found 1 Document (1.612 seconds)

BMRB: 5286

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. calmodulin, : 11 : 88 entities Detail

Related entities 2. CaM-binding sequence(299-320) from CaMKI, : 1 : 7 : 19 entities Detail

Experiments performed 5 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 4 contents Detail