BMRB: 5287

Backbone 1H and 15N Chemical shift assigments for [2H,15N]CaM:CaMKI(320) complex

Authors

Kranz, J.K., Lee, E.K., Nairn, A.C., Wand, A.JOSHUA.

Assembly

CaM:CaMKI(320) complex

Entity

1. calmodulin (polymer, Thiol state: not present), 148 monomers, 16706.21 Da Detail

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. Ca2+/CaM-dependent (rat) protein kinase I (polymer, Thiol state: not reported), 340 monomers, 38522.43 Da Detail

MGSSHHHHHH SSGLVPRGSH MPGAVEGPRW KQAEDIRDIY DFRDVLGTGA FSEVILAEDK RTQKLVAIKC IAKKALEGKE GSMENEIAVL HKIKHPNIVA LDDIYESGGH LYLIMQLVSG GELFDRIVEK GFYTERDASR LIFQVLDAVK YLHDLGIVHR DLKPENLLYY SLDEDSKIMI SDFGLSKMED PGSVLSTACG TPGYVAPEVL AQKPYSKAVD CWSIGVIAYI LLCGYPPFYD ENDAKLFEQI LKAEYEFDSP YWDDISDSAK DFIRHLMEKD PEKRFTCEQA LQHPWIAGDT ALDKNIHQSV SEQIKKNFAK SKWKQAFNAT AVVRHMRKLQ

Total weight

55228.64 Da

Max. entity weight

38522.43 Da

Source organism

Gallus gallus , Rattus norvegicus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 31.1 %, Completeness: 10.5 %, Completeness (bb): 21.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	10.5 % (365 of 3461)	7.3 % (216 of 2953)	29.3 % (149 of 508)
Backbone	21.5 % (315 of 1465)	16.7 % (166 of 993)	31.6 % (149 of 472)
Sidechain	2.6 % (51 of 1996)	2.6 % (51 of 1960)	0.0 % (0 of 36)
Aromatic	0.0 % (0 of 238)	0.0 % (0 of 233)	0.0 % (0 of 5)
Methyl	3.6 % (9 of 253)	3.6 % (9 of 253)

1. calmodulin

ADQLTEEQIA EFKEAFSLFD KDGDGTITTK ELGTVMRSLG QNPTEAELQD MINEVDADGN GTIDFPEFLT MMARKMKDTD SEEEIREAFR VFDKDGNGYI SAAELRHVMT NLGEKLTDEE VDEMIREADI DGDGQVNYEE FVQMMTAK

2. Ca2+/CaM-dependent (rat) protein kinase I

MGSSHHHHHH SSGLVPRGSH MPGAVEGPRW KQAEDIRDIY DFRDVLGTGA FSEVILAEDK RTQKLVAIKC IAKKALEGKE GSMENEIAVL HKIKHPNIVA LDDIYESGGH LYLIMQLVSG GELFDRIVEK GFYTERDASR LIFQVLDAVK YLHDLGIVHR DLKPENLLYY SLDEDSKIMI SDFGLSKMED PGSVLSTACG TPGYVAPEVL AQKPYSKAVD CWSIGVIAYI LLCGYPPFYD ENDAKLFEQI LKAEYEFDSP YWDDISDSAK DFIRHLMEKD PEKRFTCEQA LQHPWIAGDT ALDKNIHQSV SEQIKKNFAK SKWKQAFNAT AVVRHMRKLQ

Show sample condition

Sample

Temperature 303 (±0.5) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	calmodulin	[U-99% 2H; U-99% 15N]		1.0 mM
2	Ca2+/CaM-dependent (rat) protein kinase I			1.2 mM

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Release date

2002-06-12

Citation

A direct test of the reductionist approach to structural studies of calmodulin activity: relevance of peptide models of target proteins
Kranz, J.K., Lee, E.K., Nairn, A.C., Wand, A.JOSHUA.
J. Biol. Chem. (2002), 277, 16351-16354, PubMed 11904288 , DOI 10.1074/jbc.C200139200 , Abstract

Related entities 1. calmodulin, : 1 : 111 : 6 : 3 : 178 entities Detail

Related entities 2. Ca2+/CaM-dependent (rat) protein kinase I, : 2 : 91 entities Detail

Interaction partners 1. calmodulin, : 1 interactors Detail

Experiments performed 1 experiments Detail

Chemical shift validation 4 contents Detail