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Found 1 Document (3.744 seconds)

BMRB: 5293

1BTA

NMR Identification and Characterization of the Flexible Regions in the 160 KD Molten Globule-like Aggregate of Barstar at Low pH

Authors

Juneja, J., Bhavesh, N.S., Udgaonkar, J.B., Hosur, R.V.

Assembly

Barstar pH 2.7 160 kD aggregate

Entity

1. Barstar pH 2.7 160 kD aggregate (polymer, Thiol state: all free), 89 monomers, 10211.46 × 16 Da Detail

KKAVINGEQI RSISDLHQTL KKELALPEYY GENLDALWDC LTGWVEYPLV LEWRQFEQSK QLTENGAESV LQVFREAKAE GCDITIILS

Total weight

163383.36 Da

Max. entity weight

10211.46 Da

Source organism

Bacillus amyloliquefaciens

Exptl. method

NMR

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 25.8 %, Completeness: 18.9 %, Completeness (bb): 23.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	18.9 % (202 of 1068)	22.5 % (125 of 555)	12.8 % (53 of 414)	24.2 % (24 of 99)
Backbone	23.2 % (123 of 530)	26.0 % (47 of 181)	20.2 % (53 of 262)	26.4 % (23 of 87)
Sidechain	15.1 % (94 of 622)	20.9 % (78 of 374)	6.4 % (15 of 236)	8.3 % (1 of 12)
Aromatic	0.0 % (0 of 84)	0.0 % (0 of 42)	0.0 % (0 of 39)	0.0 % (0 of 3)
Methyl	9.8 % (11 of 112)	17.9 % (10 of 56)	1.8 % (1 of 56)

1. Barstar

KKAVINGEQI RSISDLHQTL KKELALPEYY GENLDALWDC LTGWVEYPLV LEWRQFEQSK QLTENGAESV LQVFREAKAE GCDITIILS

Show sample condition

Sample #1

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1), Details Lyophilized protein was dissolved in 90% H2O/ 10% D2O to a final pH of 2.7 to form the A form of barstar. The samples were equilibrated at pH 2.7 for at least 10 hours before collecting spectra.

#	Name	Isotope labeling	Type	Concentration
1	Barstar	[U-90% 15N]		1.2 mM

Sample #2

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Barstar	[U-95% 13C; U-90% 15N]		1.2 mM

Sample #3

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Barstar			1.2 mM

Protein Blocks Logo

Calculated from 1 models in PDB: 1BTA, Strand ID: A Detail

Coupling constant

17 J values in 1 lists
Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1) Detail

Release date

2002-10-30

Citation

NMR identification and characterization of the flexible regions in the 160 kDa molten globule-like aggregate of barstar at low pH
Juneja, J., Bhavesh, N.S., Udgaonkar, J.B., Hosur, R.V.
Biochemistry (2002), 41, 9885-9899, PubMed 12146954 , DOI: , Abstract

Related entities 1. Barstar pH 2.7 160 kD aggregate, : 1 : 4 : 1 : 15 : 7 entities Detail

Interaction partners 1. Barstar pH 2.7 160 kD aggregate, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 14 experiments Detail

1 1H-15N HSQC

Instrument

Model Varian Unityplus (600 MHz)

Sample #1

#	Name	Isotope labeling	Type	Concentration
1	Barstar	[U-90% 15N]		1.2 mM

Sample #2

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Barstar	[U-95% 13C; U-90% 15N]		1.2 mM

Sample #3

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Barstar			1.2 mM

2 HNN

Instrument

Model Varian Unityplus (600 MHz)

Sample #1

#	Name	Isotope labeling	Type	Concentration
1	Barstar	[U-90% 15N]		1.2 mM

Sample #2

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Barstar	[U-95% 13C; U-90% 15N]		1.2 mM

Sample #3

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Barstar			1.2 mM

3 HN(C)N

Instrument

Model Varian Unityplus (600 MHz)

Sample #1

#	Name	Isotope labeling	Type	Concentration
1	Barstar	[U-90% 15N]		1.2 mM

Sample #2

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Barstar	[U-95% 13C; U-90% 15N]		1.2 mM

Sample #3

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Barstar			1.2 mM

4 1H-15N NOESY-HSQC

Instrument

Model Varian Unityplus (600 MHz)

Sample #1

#	Name	Isotope labeling	Type	Concentration
1	Barstar	[U-90% 15N]		1.2 mM

Sample #2

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Barstar	[U-95% 13C; U-90% 15N]		1.2 mM

Sample #3

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Barstar			1.2 mM

5 1H-15N HSQC-TOCSY

Instrument

Model Varian Unityplus (600 MHz)

Sample #1

#	Name	Isotope labeling	Type	Concentration
1	Barstar	[U-90% 15N]		1.2 mM

Sample #2

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Barstar	[U-95% 13C; U-90% 15N]		1.2 mM

Sample #3

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Barstar			1.2 mM

6 1H-15N TOCSY-HSQC

Instrument

Model Varian Unityplus (600 MHz)

Sample #1

#	Name	Isotope labeling	Type	Concentration
1	Barstar	[U-90% 15N]		1.2 mM

Sample #2

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Barstar	[U-95% 13C; U-90% 15N]		1.2 mM

Sample #3

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Barstar			1.2 mM

7 HNCA

Instrument

Model Varian Unityplus (600 MHz)

Sample #1

#	Name	Isotope labeling	Type	Concentration
1	Barstar	[U-90% 15N]		1.2 mM

Sample #2

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Barstar	[U-95% 13C; U-90% 15N]		1.2 mM

Sample #3

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Barstar			1.2 mM

8 HN(CO)CA

Instrument

Model Varian Unityplus (600 MHz)

Sample #1

#	Name	Isotope labeling	Type	Concentration
1	Barstar	[U-90% 15N]		1.2 mM

Sample #2

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Barstar	[U-95% 13C; U-90% 15N]		1.2 mM

Sample #3

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Barstar			1.2 mM

9 HNCO

Instrument

Model Varian Unityplus (600 MHz)

Sample #1

#	Name	Isotope labeling	Type	Concentration
1	Barstar	[U-90% 15N]		1.2 mM

Sample #2

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Barstar	[U-95% 13C; U-90% 15N]		1.2 mM

Sample #3

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Barstar			1.2 mM

10 CBCANH

Instrument

Model Varian Unityplus (600 MHz)

Sample #1

#	Name	Isotope labeling	Type	Concentration
1	Barstar	[U-90% 15N]		1.2 mM

Sample #2

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Barstar	[U-95% 13C; U-90% 15N]		1.2 mM

Sample #3

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Barstar			1.2 mM

11 CBCA(CO)NH

Instrument

Model Varian Unityplus (600 MHz)

Sample #1

#	Name	Isotope labeling	Type	Concentration
1	Barstar	[U-90% 15N]		1.2 mM

Sample #2

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Barstar	[U-95% 13C; U-90% 15N]		1.2 mM

Sample #3

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Barstar			1.2 mM

12 HNHA

Instrument

Model Varian Unityplus (600 MHz)

Sample #1

#	Name	Isotope labeling	Type	Concentration
1	Barstar	[U-90% 15N]		1.2 mM

Sample #2

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Barstar	[U-95% 13C; U-90% 15N]		1.2 mM

Sample #3

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Barstar			1.2 mM

13 1H-1H TOCSY

Instrument

Model Varian Unityplus (600 MHz)

Sample #1

#	Name	Isotope labeling	Type	Concentration
1	Barstar	[U-90% 15N]		1.2 mM

Sample #2

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Barstar	[U-95% 13C; U-90% 15N]		1.2 mM

Sample #3

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Barstar			1.2 mM

14 1H-1H NOESY

Instrument

Model Varian Unityplus (600 MHz)

Sample #1

#	Name	Isotope labeling	Type	Concentration
1	Barstar	[U-90% 15N]		1.2 mM

Sample #2

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Barstar	[U-95% 13C; U-90% 15N]		1.2 mM

Sample #3

Pressure 760 mmHg, Temperature 298 (±0.1) K, pH 2.7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
3	Barstar			1.2 mM

nullKeywords Barstar, low pH, 160 kD aggregate, HNN, HN(C)N

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Found 1 Document (3.744 seconds)

BMRB: 5293

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Related entities 1. Barstar pH 2.7 160 kD aggregate, : 1 : 4 : 1 : 15 : 7 entities Detail

Interaction partners 1. Barstar pH 2.7 160 kD aggregate, : 2 interactors Detail

Experiments performed 14 experiments Detail

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