Structure of the beta subunit of translation initiation factor 2 from the archaeon Methanococcus jannaschii: A representative of the eIF2beta/eIF5 family of proteins
MSDLENIDYY DYKALLKRAR SQIPDYVFQK DRFELPEIEI LIEGNRTIIR NFRELAKAVN RDEEFFAKYL LKETGSAGNL EGGRLILQRR ISPELLKSRI NDFLREYVIC RECGKPDTKI IKEGRVHLLK CMACGAIRPI RMI
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | na | sing | 1:CYS110:SG | 2:ZN1:ZN |
2 | na | sing | 1:CYS113:SG | 2:ZN1:ZN |
3 | na | sing | 1:CYS131:SG | 2:ZN1:ZN |
4 | na | sing | 1:CYS134:SG | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 43.2 % (762 of 1763) | 46.1 % (431 of 934) | 32.4 % (221 of 682) | 74.8 % (110 of 147) |
Backbone | 64.9 % (550 of 848) | 78.5 % (227 of 289) | 50.6 % (213 of 421) | 79.7 % (110 of 138) |
Sidechain | 21.7 % (228 of 1050) | 31.6 % (204 of 645) | 6.1 % (24 of 396) | 0.0 % (0 of 9) |
Aromatic | 4.5 % (5 of 112) | 8.9 % (5 of 56) | 0.0 % (0 of 56) | |
Methyl | 33.1 % (55 of 166) | 57.8 % (48 of 83) | 8.4 % (7 of 83) |
1. translation initiation factor IF2b
MSDLENIDYY DYKALLKRAR SQIPDYVFQK DRFELPEIEI LIEGNRTIIR NFRELAKAVN RDEEFFAKYL LKETGSAGNL EGGRLILQRR ISPELLKSRI NDFLREYVIC RECGKPDTKI IKEGRVHLLK CMACGAIRPI RMITemperature 303 (±1) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | translation initiation factor IF2b | [U-13C; U-15N] | 0.5 ~ 1.5 mM | |
2 | ZINC (II) ION | 0.0 ~ 0.0 mM |
Temperature 303 (±1) K, pH 6.0 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | translation initiation factor IF2b | [U-15N] | 0.5 ~ 1.5 mM | |
4 | ZINC (II) ION | 0.0 ~ 0.0 mM |