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Found 1 Document (1.645 seconds)

BMRB: 5294

1K8B

Structure of the beta subunit of translation initiation factor 2 from the archaeon Methanococcus jannaschii: A representative of the eIF2beta/eIF5 family of proteins

Authors

Cho, S., Hoffman, D.W.

Assembly

beta subunit of translation initiation factor 2

Entity

1. translation initiation factor IF2b (polymer, Thiol state: all other bound), 143 monomers, 16796.43 Da Detail

MSDLENIDYY DYKALLKRAR SQIPDYVFQK DRFELPEIEI LIEGNRTIIR NFRELAKAVN RDEEFFAKYL LKETGSAGNL EGGRLILQRR ISPELLKSRI NDFLREYVIC RECGKPDTKI IKEGRVHLLK CMACGAIRPI RMI

2. ZN (non-polymer), 65.409 Da

Total weight

16861.838 Da

Max. entity weight

16796.43 Da

Entity Connection

na 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS110:SG	2:ZN1:ZN
2	na	sing	1:CYS113:SG	2:ZN1:ZN
3	na	sing	1:CYS131:SG	2:ZN1:ZN
4	na	sing	1:CYS134:SG	2:ZN1:ZN

Source organism

Methanocaldococcus jannaschii

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 81.8 %, Completeness: 43.2 %, Completeness (bb): 64.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	43.2 % (762 of 1763)	46.1 % (431 of 934)	32.4 % (221 of 682)	74.8 % (110 of 147)
Backbone	64.9 % (550 of 848)	78.5 % (227 of 289)	50.6 % (213 of 421)	79.7 % (110 of 138)
Sidechain	21.7 % (228 of 1050)	31.6 % (204 of 645)	6.1 % (24 of 396)	0.0 % (0 of 9)
Aromatic	4.5 % (5 of 112)	8.9 % (5 of 56)	0.0 % (0 of 56)
Methyl	33.1 % (55 of 166)	57.8 % (48 of 83)	8.4 % (7 of 83)

1. translation initiation factor IF2b

MSDLENIDYY DYKALLKRAR SQIPDYVFQK DRFELPEIEI LIEGNRTIIR NFRELAKAVN RDEEFFAKYL LKETGSAGNL EGGRLILQRR ISPELLKSRI NDFLREYVIC RECGKPDTKI IKEGRVHLLK CMACGAIRPI RMI

Show sample condition

Sample #1

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	translation initiation factor IF2b	[U-13C; U-15N]		0.5 ~ 1.5 mM
2	ZINC (II) ION			0.0 ~ 0.0 mM

Sample #2

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	translation initiation factor IF2b	[U-15N]		0.5 ~ 1.5 mM
4	ZINC (II) ION			0.0 ~ 0.0 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 1K8B, Strand ID: A Detail

Release date

2002-09-10

Citation

Structure of the beta subunit of translation initiation factor 2 from the archaeon Methanococcus jannaschii: a representative of the eIF2beta/eIF5 family of proteins
Cho, S., Hoffman, D.W.
Biochemistry (2002), 41, 5730-5742, PubMed 11980477 , DOI: , Abstract

Related entities 1. translation initiation factor IF2b, : 2 : 93 entities Detail

Experiments performed 8 experiments Detail

1 HNCA

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	translation initiation factor IF2b	[U-13C; U-15N]		0.5 ~ 1.5 mM
2	ZINC (II) ION			0.0 ~ 0.0 mM

Sample #2

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	translation initiation factor IF2b	[U-15N]		0.5 ~ 1.5 mM
4	ZINC (II) ION			0.0 ~ 0.0 mM

2 HNCO

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	translation initiation factor IF2b	[U-13C; U-15N]		0.5 ~ 1.5 mM
2	ZINC (II) ION			0.0 ~ 0.0 mM

Sample #2

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	translation initiation factor IF2b	[U-15N]		0.5 ~ 1.5 mM
4	ZINC (II) ION			0.0 ~ 0.0 mM

3 HNCACB

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	translation initiation factor IF2b	[U-13C; U-15N]		0.5 ~ 1.5 mM
2	ZINC (II) ION			0.0 ~ 0.0 mM

Sample #2

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	translation initiation factor IF2b	[U-15N]		0.5 ~ 1.5 mM
4	ZINC (II) ION			0.0 ~ 0.0 mM

4 HN(CO)CACB

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	translation initiation factor IF2b	[U-13C; U-15N]		0.5 ~ 1.5 mM
2	ZINC (II) ION			0.0 ~ 0.0 mM

Sample #2

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	translation initiation factor IF2b	[U-15N]		0.5 ~ 1.5 mM
4	ZINC (II) ION			0.0 ~ 0.0 mM

5 3D-HCCH-TOCSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	translation initiation factor IF2b	[U-13C; U-15N]		0.5 ~ 1.5 mM
2	ZINC (II) ION			0.0 ~ 0.0 mM

Sample #2

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	translation initiation factor IF2b	[U-15N]		0.5 ~ 1.5 mM
4	ZINC (II) ION			0.0 ~ 0.0 mM

6 15N-HMQC

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	translation initiation factor IF2b	[U-13C; U-15N]		0.5 ~ 1.5 mM
2	ZINC (II) ION			0.0 ~ 0.0 mM

Sample #2

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	translation initiation factor IF2b	[U-15N]		0.5 ~ 1.5 mM
4	ZINC (II) ION			0.0 ~ 0.0 mM

7 3D 1H-1H-15N NOESY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	translation initiation factor IF2b	[U-13C; U-15N]		0.5 ~ 1.5 mM
2	ZINC (II) ION			0.0 ~ 0.0 mM

Sample #2

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	translation initiation factor IF2b	[U-15N]		0.5 ~ 1.5 mM
4	ZINC (II) ION			0.0 ~ 0.0 mM

8 3D 1H-13C-1H HCCH-TOCSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	translation initiation factor IF2b	[U-13C; U-15N]		0.5 ~ 1.5 mM
2	ZINC (II) ION			0.0 ~ 0.0 mM

Sample #2

Temperature 303 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	translation initiation factor IF2b	[U-15N]		0.5 ~ 1.5 mM
4	ZINC (II) ION			0.0 ~ 0.0 mM

nullKeywords translation initiation factor, protein structure, aIf2b

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Found 1 Document (1.645 seconds)

BMRB: 5294

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Related entities 1. translation initiation factor IF2b, : 2 : 93 entities Detail

Experiments performed 8 experiments Detail

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