Two-Dimensional NMR Studies of Staphylococcal Nuclease: Evidence for Conformational Heterogeneity from Hydrogen-1, Carbon-13, and Nitrogen-15 Spin System Assignments of the Aromatic Amino Acids in the Nuclease H124L-Thymidine 3',5'-Bisphosp
ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 14.5 % (250 of 1729) | 13.2 % (120 of 912) | 17.6 % (118 of 669) | 8.1 % (12 of 148) |
Backbone | 12.1 % (102 of 846) | 10.4 % (30 of 289) | 14.3 % (60 of 420) | 8.8 % (12 of 137) |
Sidechain | 17.0 % (173 of 1017) | 14.6 % (91 of 623) | 21.4 % (82 of 383) | 0.0 % (0 of 11) |
Aromatic | 96.4 % (106 of 110) | 98.2 % (54 of 55) | 96.3 % (52 of 54) | 0.0 % (0 of 1) |
Methyl | 10.0 % (15 of 150) | 9.3 % (7 of 75) | 10.7 % (8 of 75) |
1. micrococcal nuclease
ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SEDTemperature 318 K, pH 5.5