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Two-Dimensional NMR Studies of Staphylococcal Nuclease: Evidence for Conformational Heterogeneity from Hydrogen-1, Carbon-13, and Nitrogen-15 Spin System Assignments of the Aromatic Amino Acids in the Nuclease H124L-Thymidine 3',5'-Bisphosp
Authors
Wang, J., Hinck, A.P., Loh, S.N., Markley, J.L.
Assembly
micrococcal nuclease
Entity
1. micrococcal nuclease (polymer), 143 monomers, 16214.55 Da Detail

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED


Formula weight
16214.55 Da
Source organism
Staphylococcus aureus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 10.5 %, Completeness: 9.9 %, Completeness (bb): 4.8 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All 9.9 % (172 of 1734)10.3 % (94 of 912)11.5 % (77 of 669) 0.7 % (1 of 153)
Backbone 4.8 % (40 of 837) 5.0 % (14 of 280) 6.0 % (25 of 420) 0.7 % (1 of 137)
Sidechain14.0 % (144 of 1031)12.7 % (80 of 632)16.7 % (64 of 383) 0.0 % (0 of 16)
Aromatic96.4 % (106 of 110)98.2 % (54 of 55)96.3 % (52 of 54) 0.0 % (0 of 1)
Methyl 0.0 % (0 of 150) 0.0 % (0 of 75) 0.0 % (0 of 75)

1. micrococcal nuclease

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED

Sample

Temperature 318 K, pH 5.5



Release date
1995-07-30
Citation
Two-dimensional NMR studies of staphylococcal nuclease: evidence for conformational heterogeneity from hydrogen-1, carbon-13, and nitrogen-15 spin system assignments of the aromatic amino acids in the nuclease H124L-thymidine 3',5'-bisphosphate-Ca2+ ternary complex
Wang, J., Hinck, A.P., Loh, S.N., Markley, J.L.
Biochemistry (1990), 29, 4242-4253, PubMed 2361141 , DOI 10.1021/bi00469a029 ,
Related entities 1. micrococcal nuclease, : 1 : 7 : 43 : 73 entities Detail
Experiments performed 1 experiments Detail