BMRB: 5310

Backbone and Sidechain 1H, 13C and 15N resonance assignments for PLC-gamma 1 C-terminal SH2 domain complexed with a PDGFR-derived phosphopeptide

Authors

Forman-Kay, J.D.

Assembly

phospholipase C gamma-1 C-terminal SH2 domain

Entity

1. PLCC SH2 (polymer, Thiol state: all free), 105 monomers, 12254.75 Da Detail

GSPGIHESKE WYHASLTRAQ AEHMLMRVPR DGAFLVRKRN EPNSYAISFR AEGKIKHCRV QQEGQTVMLG NSEFDSLVDL ISYYEKHPLY RKMKLRYPIN EENSS

2. PDGFR-derived phosphopeptide, residues 1018 to 1029 (polymer, Thiol state: not present), 12 monomers, 1479.523 Da Detail

DNDXIIPLPD PK

Total weight

13734.273 Da

Max. entity weight

12254.75 Da

Source organism

Bos taurus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 88.0 %, Completeness: 82.8 %, Completeness (bb): 87.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.8 % (1152 of 1392)	80.2 % (591 of 737)	84.7 % (454 of 536)	89.9 % (107 of 119)
Backbone	87.9 % (598 of 680)	87.8 % (202 of 230)	87.1 % (298 of 342)	90.7 % (98 of 108)
Sidechain	79.3 % (652 of 822)	76.7 % (389 of 507)	83.6 % (254 of 304)	81.8 % (9 of 11)
Aromatic	64.5 % (71 of 110)	63.6 % (35 of 55)	64.8 % (35 of 54)	100.0 % (1 of 1)
Methyl	87.0 % (87 of 100)	86.0 % (43 of 50)	88.0 % (44 of 50)

1. PLCC SH2

GSPGIHESKE WYHASLTRAQ AEHMLMRVPR DGAFLVRKRN EPNSYAISFR AEGKIKHCRV QQEGQTVMLG NSEFDSLVDL ISYYEKHPLY RKMKLRYPIN EENSS

2. PDGFR-derived phosphopeptide, residues 1018 to 1029

DNDXIIPLPD PK

Show sample condition

Sample

Temperature 303 (±1) K, pH 6.4 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	PLCC SH2	[U-99% 13C; U-99% 15N]		1.5 mM
2	PDGFR-derived phosphopeptide, residues 1018 to 1029			1.5 mM

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Chem. Shift Complete2

Sequence coverage: 35.9 %, Completeness: 51.3 %, Completeness (bb): 55.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	51.3 % (1427 of 2784)	51.8 % (764 of 1474)	49.3 % (529 of 1072)	56.3 % (134 of 238)
Backbone	55.2 % (751 of 1360)	57.8 % (266 of 460)	52.9 % (362 of 684)	56.9 % (123 of 216)
Sidechain	48.4 % (796 of 1644)	49.1 % (498 of 1014)	47.2 % (287 of 608)	50.0 % (11 of 22)
Aromatic	34.1 % (75 of 220)	35.5 % (39 of 110)	32.4 % (35 of 108)	50.0 % (1 of 2)
Methyl	52.0 % (104 of 200)	55.0 % (55 of 100)	49.0 % (49 of 100)

1. PLCC SH2

GSPGIHESKE WYHASLTRAQ AEHMLMRVPR DGAFLVRKRN EPNSYAISFR AEGKIKHCRV QQEGQTVMLG NSEFDSLVDL ISYYEKHPLY RKMKLRYPIN EENSS

2. PDGFR-derived phosphopeptide, residues 1018 to 1029

DNDXIIPLPD PK

Show sample condition

Sample

Temperature 303 (±1) K, pH 6.4 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	PLCC SH2	[U-99% 13C; U-99% 15N]		1.5 mM
2	PDGFR-derived phosphopeptide, residues 1018 to 1029			1.5 mM

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Protein Blocks Logo

Calculated from 1 models in PDB: 2PLD, Strand ID: A, B Detail

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Release date

2008-07-16

Citation 1

Nuclear magnetic resonance structure of an SH2 domain of phospholipase C-gamma 1 complexed with a high affinity binding peptide
Pascal, S.M., Singer, A.U., Gish, G., Yamazaki, T., Shoelson, S.E., Pawson, T., Kay, L.E., Forman-Kay, J.D.
Cell (1994), 77, 461-472, PubMed 8181064 , Abstract

Show more 3 citaions

Citation 2

Sequence similarity of phospholipase C with the non-catalytic region of src
Stahl, M.L., Ferenz, C.R., Kelleher, K.L., Kriz, R.W., Knopf, J.L.
Nature (1988), 332, 269-272, PubMed 2831461 , DOI 10.1038/332269a0 , Abstract

Citation 3

Phosphatidylinositol 3-kinase p85 SH2 domain specificity defined by direct phosphopeptide/SH2 domain binding
Piccione, E., Case, R.D., Domchek, S.M., Hu, P., Chaudhuri, M., Backer, J.M., Schlessinger, J., Shoelson, S.E.
Biochemistry (1993), 32, 3197-3202, PubMed 8384875 , Abstract

Citation 4

[The history of the discovery of anesthesia. Progress is the attainment of utopia]
Miguel Giacchella, R.
Rev. Soc. Odontol. La Plata (1988), 1, 29-30, PubMed 3078736 , Abstract

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Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. PLCC SH2, : 7 : 179 entities Detail

Related entities 2. PDGFR-derived phosphopeptide, residues 1018 to 1029, : 1 : 1 : 7 entities Detail

Interaction partners 1. PLCC SH2, : 28 interactors Detail

Interaction partners 2. PDGFR-derived phosphopeptide, residues 1018 to 1029, : 1 interactors Detail

Experiments performed 8 experiments Detail