BMRBj - BMREntryMaster

Found 1 Document (2.223 seconds)

BMRB: 5316

1L6N

Structure of the N-terminal 283-Residue Fragment of the HIV-1 Gag Polyprotein

Authors

Tang, C., Ndassa, Y., Summers, M.F.

Assembly

gag

Entity

1. gag (polymer, Thiol state: all free), 288 monomers, 32216.29 Da Detail

GARASVLSGG ELDKWEKIRL RPGGKKQYKL KHIVWASREL ERFAVNPGLL ETSEGCRQIL GQLQPSLQTG SEELRSLYNT IAVLYCVHQR IDVKDTKEAL DKIEEEQNKS KKKAQQAAAD TGNNSQVSQN YPIVQNLQGQ MVHQAISPRT LNAWVKVVEE KAFSPEVIPM FSALSEGATP QDLNTMLNTV GGHQAAMQML KETINEEAAE WDRLHPVHAG PIAPGQMREP RGSDIAGTTS TLQEQIGWMT HNPPIPVGEI YKRWIILGLN KIVRMYSPTS ILHHHHHH

Formula weight

32216.29 Da

Source organism

Human immunodeficiency virus

Exptl. method

NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 98.3 %, Completeness: 81.7 %, Completeness (bb): 94.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.7 % (2754 of 3370)	76.5 % (1350 of 1765)	87.6 % (1133 of 1293)	86.9 % (271 of 312)
Backbone	94.5 % (1603 of 1696)	95.0 % (552 of 581)	94.0 % (792 of 843)	95.2 % (259 of 272)
Sidechain	71.7 % (1391 of 1941)	67.1 % (794 of 1184)	81.7 % (586 of 717)	27.5 % (11 of 40)
Aromatic	44.6 % (90 of 202)	47.5 % (48 of 101)	37.9 % (36 of 95)	100.0 % (6 of 6)
Methyl	96.9 % (308 of 318)	96.2 % (153 of 159)	97.5 % (155 of 159)

1. matrix-capsid

Show sample condition

Sample

Temperature 303.3 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	matrix-capsid	[U-100% 13C; U-100% 15N]	1.0 mM
2	sodium acetate		50 mM
3	NaCl		100 mM
4	beta-mecaptoethanol		5 mM

Chem. Shift Complete2

Sequence coverage: 37.2 %, Completeness: 52.0 %, Completeness (bb): 60.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	52.0 % (3505 of 6740)	48.4 % (1709 of 3530)	55.5 % (1434 of 2586)	58.0 % (362 of 624)
Backbone	60.4 % (2050 of 3392)	59.6 % (693 of 1162)	60.0 % (1012 of 1686)	63.4 % (345 of 544)
Sidechain	45.6 % (1772 of 3882)	42.7 % (1012 of 2368)	51.9 % (744 of 1434)	20.0 % (16 of 80)
Aromatic	27.2 % (110 of 404)	30.7 % (62 of 202)	22.1 % (42 of 190)	50.0 % (6 of 12)
Methyl	63.4 % (403 of 636)	62.9 % (200 of 318)	63.8 % (203 of 318)

1. matrix-capsid

Show sample condition

Sample

Temperature 303.3 (±0.5) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Concentration
1	matrix-capsid	[U-100% 13C; U-100% 15N]	1.0 mM
2	sodium acetate		50 mM
3	NaCl		100 mM
4	beta-mecaptoethanol		5 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 1L6N, Strand ID: A Detail

Release date

2002-03-10

Citation

Structure of the N-terminal 283-residue fragment of the immature HIV-1 Gag polyprotein
Tang, C., Ndassa, Y., Summers, M.F.
Nat. Struct. Biol. (2002), 9, 537-543, PubMed 12032547 , DOI 10.1038/nsb806 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P12497 released on 1989-10-01
Title POL_HV1N5 Entity Gag-Pol polyprotein

Related entities 1. gag, : 1 : 3 : 101 entities Detail

Interaction partners 1. gag, : 4 interactors Detail

More details for 4 interactors identified by 3 citations

Experiments performed 11 experiments Detail

1 1H-15N HSQC