BMRB: 5318

Backbone and Sidechain 1H, 13C and 15N resonance assignments for PLC-gamma 1 C-terminal SH2 domain

Authors

Forman-Kay, J.D.

Assembly

phospholipase C gamma-1 C-terminal SH2 domain

Entity

1. phospholipase C gamma-1 C-terminal SH2 domain (polymer, Thiol state: all free), 105 monomers, 12254.75 Da Detail

GSPGIHESKE WYHASLTRAQ AEHMLMRVPR DGAFLVRKRN EPNSYAISFR AEGKIKHCRV QQEGQTVMLG NSEFDSLVDL ISYYEKHPLY RKMKLRYPIN EENSS

Formula weight

12254.75 Da

Source organism

Bos taurus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.3 %, Completeness: 29.3 %, Completeness (bb): 64.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	29.3 % (228 of 777)	19.5 % (130 of 667)	89.1 % (98 of 110)
Backbone	64.6 % (201 of 311)	48.8 % (103 of 211)	98.0 % (98 of 100)
Sidechain	5.8 % (27 of 466)	5.9 % (27 of 456)	0.0 % (0 of 10)
Aromatic	0.0 % (0 of 56)	0.0 % (0 of 55)	0.0 % (0 of 1)
Methyl	2.3 % (1 of 44)	2.3 % (1 of 44)

1. PLCC SH2

GSPGIHESKE WYHASLTRAQ AEHMLMRVPR DGAFLVRKRN EPNSYAISFR AEGKIKHCRV QQEGQTVMLG NSEFDSLVDL ISYYEKHPLY RKMKLRYPIN EENSS

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Sample

Temperature 303 (±1) K, pH 6.4 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	PLCC SH2	[U-99% 15N]		1.5 mM
2	sodium phosphate			100 mM

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Protein Blocks Logo

Calculated from 1 models in PDB: 2PLD, Strand ID: A Detail

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Release date

2002-04-30

Citation 1

Nuclear magnetic resonance structure of an SH2 domain of phospholipase C-gamma 1 complexed with a high affinity binding peptide
Pascal, S.M., Gish, G., Yamazaki, T., Singer, A.U., Shoelson, S.E., Pawson, T., Kay, L.E., Forman-Kay, J.D.
Cell (1994), 77, 461-472, PubMed 8181064 , Abstract

Show more 3 citaions

Citation 2

Sequence similarity of phospholipase C with the non-catalytic region of src
Nature (1988), 332, 269-272, PubMed 2831461 , DOI 10.1038/332269a0 , Abstract

Citation 3

Phosphatidylinositol 3-kinase p85 SH2 domain specificity defined by direct phosphopeptide/SH2 domain binding
Biochemistry (1993), 32, 3197-3202, PubMed 8384875 , Abstract

Citation 4

[The history of the discovery of anesthesia. Progress is the attainment of utopia]
Rev. Soc. Odontol. La Plata (1988), 1, 29-30, PubMed 3078736 , Abstract

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Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

Related entities 1. phospholipase C gamma-1 C-terminal SH2 domain, : 1 : 2 : 17 : 323 entities Detail

Interaction partners 1. phospholipase C gamma-1 C-terminal SH2 domain, : 28 interactors Detail

Experiments performed 8 experiments Detail