Structural basis for Hif-1alpha/CBP recognition in the cellular hypoxic response
ADPEKRKLIQ QQLVLLLHAH KCQRREQANG EVRACSLPHC RTMKNVLNHM THCQAGKACQ VAHCASSRQI ISHWKNCTRH DCPVCLPLKN ASDKR
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 2:CYS22:SG | 3:ZN1:ZN |
2 | disulfide | sing | 2:CYS35:SG | 3:ZN1:ZN |
3 | disulfide | sing | 2:CYS40:SG | 3:ZN1:ZN |
4 | disulfide | sing | 2:CYS53:SG | 3:ZN1:ZN |
5 | disulfide | sing | 2:CYS59:SG | 3:ZN1:ZN |
6 | disulfide | sing | 2:CYS64:SG | 3:ZN1:ZN |
7 | disulfide | sing | 2:CYS77:SG | 3:ZN1:ZN |
8 | disulfide | sing | 2:CYS82:SG | 3:ZN1:ZN |
9 | disulfide | sing | 2:CYS85:SG | 3:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 45.1 % (754 of 1670) | 45.8 % (403 of 880) | 42.7 % (268 of 628) | 51.2 % (83 of 162) |
Backbone | 45.6 % (394 of 864) | 51.0 % (149 of 292) | 39.6 % (171 of 432) | 52.9 % (74 of 140) |
Sidechain | 45.7 % (432 of 946) | 43.2 % (254 of 588) | 50.3 % (169 of 336) | 40.9 % (9 of 22) |
Aromatic | 9.6 % (5 of 52) | 11.5 % (3 of 26) | 8.0 % (2 of 25) | 0.0 % (0 of 1) |
Methyl | 63.8 % (97 of 152) | 64.5 % (49 of 76) | 63.2 % (48 of 76) |
1. hypoxia induced factor 1 alpha
SDLACRLLGQ SMDESGLPQL TSYDCEVNAP IQGSRNLLQG EELLRALDQV N2. CREB-binding protein
ADPEKRKLIQ QQLVLLLHAH KCQRREQANG EVRACSLPHC RTMKNVLNHM THCQAGKACQ VAHCASSRQI ISHWKNCTRH DCPVCLPLKN ASDKRTemperature 298 (±1) K, pH 6.1 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | hypoxia induced factor 1 alpha | 2 mM | ||
2 | CREB-binding protein | [U-13C; U-15N] | 1 mM | |
3 | ZINC (II) ION | 3 mM | ||
4 | D-TRIS | 10 mM | ||
5 | D-DTT | 10 mM | ||
6 | NaN3 | 0.02 % | ||
7 | ZNSO4 | 0.5 mM | ||
8 | H2O | 90 % | ||
9 | D2O | 10 % |
Temperature 298 (±1) K, pH 6.1 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | hypoxia induced factor 1 alpha | 2 mM | ||
11 | CREB-binding protein | [U-13C; U-15N] | 1 mM | |
12 | ZINC (II) ION | 3 mM | ||
13 | D-TRIS | 10 mM | ||
14 | D-DTT | 10 mM | ||
15 | NaN3 | 0.02 % | ||
16 | ZNSO4 | 0.5 mM | ||
17 | D2O | 100 % |
Temperature 298 (±1) K, pH 6.1 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
18 | hypoxia induced factor 1 alpha | 2 mM | ||
19 | CREB-binding protein | [U-15N] | 1 mM | |
20 | ZINC (II) ION | 3 mM | ||
21 | D-TRIS | 10 mM | ||
22 | D-DTT | 10 mM | ||
23 | NaN3 | 0.02 % | ||
24 | ZNSO4 | 0.5 mM | ||
25 | H2O | 90 % | ||
26 | D2O | 10 % |
Temperature 298 (±1) K, pH 6.1 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
27 | hypoxia induced factor 1 alpha | [U-13C; U-15N] | 1 mM | |
28 | CREB-binding protein | 2 mM | ||
29 | ZINC (II) ION | 6 mM | ||
30 | D-TRIS | 10 mM | ||
31 | D-DTT | 10 mM | ||
32 | NaN3 | 0.02 % | ||
33 | ZNSO4 | 0.5 mM | ||
34 | H2O | 90 % | ||
35 | D2O | 10 % |
Temperature 298 (±1) K, pH 6.1 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
36 | hypoxia induced factor 1 alpha | [U-13C; U-15N] | 1 mM | |
37 | CREB-binding protein | 2 mM | ||
38 | ZINC (II) ION | 6 mM | ||
39 | D-TRIS | 10 mM | ||
40 | D-DTT | 10 mM | ||
41 | NaN3 | 0.02 % | ||
42 | ZNSO4 | 0.5 mM | ||
43 | D2O | 100 % |
Temperature 298 (±1) K, pH 6.1 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
44 | hypoxia induced factor 1 alpha | [U-15N] | 1 mM | |
45 | CREB-binding protein | 2 mM | ||
46 | ZINC (II) ION | 6 mM | ||
47 | D-TRIS | 10 mM | ||
48 | D-DTT | 10 mM | ||
49 | NaN3 | 0.02 % | ||
50 | ZNSO4 | 0.5 mM | ||
51 | H2O | 90 % | ||
52 | D2O | 10 % |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 52.6 % (1756 of 3340) | 54.5 % (959 of 1760) | 48.9 % (614 of 1256) | 56.5 % (183 of 324) |
Backbone | 51.6 % (891 of 1728) | 58.4 % (341 of 584) | 45.0 % (389 of 864) | 57.5 % (161 of 280) |
Sidechain | 54.6 % (1033 of 1892) | 52.6 % (618 of 1176) | 58.5 % (393 of 672) | 50.0 % (22 of 44) |
Aromatic | 33.7 % (35 of 104) | 34.6 % (18 of 52) | 32.0 % (16 of 50) | 50.0 % (1 of 2) |
Methyl | 64.1 % (195 of 304) | 65.1 % (99 of 152) | 63.2 % (96 of 152) |
1. hypoxia induced factor 1 alpha
SDLACRLLGQ SMDESGLPQL TSYDCEVNAP IQGSRNLLQG EELLRALDQV N2. CREB-binding protein
ADPEKRKLIQ QQLVLLLHAH KCQRREQANG EVRACSLPHC RTMKNVLNHM THCQAGKACQ VAHCASSRQI ISHWKNCTRH DCPVCLPLKN ASDKRTemperature 298 (±1) K, pH 6.1 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | hypoxia induced factor 1 alpha | 2 mM | ||
2 | CREB-binding protein | [U-13C; U-15N] | 1 mM | |
3 | ZINC (II) ION | 3 mM | ||
4 | D-TRIS | 10 mM | ||
5 | D-DTT | 10 mM | ||
6 | NaN3 | 0.02 % | ||
7 | ZNSO4 | 0.5 mM | ||
8 | H2O | 90 % | ||
9 | D2O | 10 % |
Temperature 298 (±1) K, pH 6.1 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | hypoxia induced factor 1 alpha | 2 mM | ||
11 | CREB-binding protein | [U-13C; U-15N] | 1 mM | |
12 | ZINC (II) ION | 3 mM | ||
13 | D-TRIS | 10 mM | ||
14 | D-DTT | 10 mM | ||
15 | NaN3 | 0.02 % | ||
16 | ZNSO4 | 0.5 mM | ||
17 | D2O | 100 % |
Temperature 298 (±1) K, pH 6.1 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
18 | hypoxia induced factor 1 alpha | 2 mM | ||
19 | CREB-binding protein | [U-15N] | 1 mM | |
20 | ZINC (II) ION | 3 mM | ||
21 | D-TRIS | 10 mM | ||
22 | D-DTT | 10 mM | ||
23 | NaN3 | 0.02 % | ||
24 | ZNSO4 | 0.5 mM | ||
25 | H2O | 90 % | ||
26 | D2O | 10 % |
Temperature 298 (±1) K, pH 6.1 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
27 | hypoxia induced factor 1 alpha | [U-13C; U-15N] | 1 mM | |
28 | CREB-binding protein | 2 mM | ||
29 | ZINC (II) ION | 6 mM | ||
30 | D-TRIS | 10 mM | ||
31 | D-DTT | 10 mM | ||
32 | NaN3 | 0.02 % | ||
33 | ZNSO4 | 0.5 mM | ||
34 | H2O | 90 % | ||
35 | D2O | 10 % |
Temperature 298 (±1) K, pH 6.1 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
36 | hypoxia induced factor 1 alpha | [U-13C; U-15N] | 1 mM | |
37 | CREB-binding protein | 2 mM | ||
38 | ZINC (II) ION | 6 mM | ||
39 | D-TRIS | 10 mM | ||
40 | D-DTT | 10 mM | ||
41 | NaN3 | 0.02 % | ||
42 | ZNSO4 | 0.5 mM | ||
43 | D2O | 100 % |
Temperature 298 (±1) K, pH 6.1 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
44 | hypoxia induced factor 1 alpha | [U-15N] | 1 mM | |
45 | CREB-binding protein | 2 mM | ||
46 | ZINC (II) ION | 6 mM | ||
47 | D-TRIS | 10 mM | ||
48 | D-DTT | 10 mM | ||
49 | NaN3 | 0.02 % | ||
50 | ZNSO4 | 0.5 mM | ||
51 | H2O | 90 % | ||
52 | D2O | 10 % |