BMRB: 5332

1H, 13C, and 15N Chemical Shift Assignments for E85Q recoverin

Authors

Ames, J.B., Hamasaki, N., Molchanova, T.

Assembly

recoverin mutant, E85Q

Entity

1. Recoverin (polymer, Thiol state: all free), 202 monomers, 23412.26 Da Detail

XGNSKSGALS KEILEELQLN TKFTEEELSS WYQSFLKECP SGRITRQEFQ TIYSKFFPEA DPKAYAQHVF RSFDANSDGT LDFKEYVIAL HMTSAGKTNQ KLEWAFSLYD VDGNGTISKN EVLEIVTAIF KMISPEDTKH LPEDENTPEK RAEKIWGFFG KKDDDKLTEK EFIEGTLANK EILRLIQFEP QKVKEKLKEK KL

2. CA (non-polymer), 40.078 × 2 Da

Total weight

23492.416 Da

Max. entity weight

23412.26 Da

Source organism

Bos taurus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.1 %, Completeness: 55.3 %, Completeness (bb): 90.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	55.3 % (1362 of 2461)	47.3 % (612 of 1293)	60.0 % (573 of 955)	83.1 % (177 of 213)
Backbone	90.3 % (1076 of 1192)	89.9 % (364 of 405)	90.2 % (535 of 593)	91.2 % (177 of 194)
Sidechain	31.6 % (462 of 1460)	27.9 % (248 of 888)	38.7 % (214 of 553)	0.0 % (0 of 19)
Aromatic	4.4 % (10 of 228)	6.1 % (7 of 114)	2.7 % (3 of 111)	0.0 % (0 of 3)
Methyl	25.0 % (48 of 192)	25.0 % (24 of 96)	25.0 % (24 of 96)

1. Recoverin

XGNSKSGALS KEILEELQLN TKFTEEELSS WYQSFLKECP SGRITRQEFQ TIYSKFFPEA DPKAYAQHVF RSFDANSDGT LDFKEYVIAL HMTSAGKTNQ KLEWAFSLYD VDGNGTISKN EVLEIVTAIF KMISPEDTKH LPEDENTPEK RAEKIWGFFG KKDDDKLTEK EFIEGTLANK EILRLIQFEP QKVKEKLKEK KL

Show sample condition

Sample

Temperature 310 (±1) K, pH 6.7 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Recoverin	[U-13C; U-15N]		0.8 mM

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LACS Plot; CA

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.08 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.01 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: -0.34 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 24 models in PDB: 1JSA, Strand ID: A Detail

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Release date

2002-06-12

Citation

Structure and calcium-binding studies of a recoverin mutant (E85Q) in an allosteric intermediate state
Ames, J.B., Hamasaki, N., Molchanova, T.
Biochemistry (2002), 41, 5776-5787, PubMed 11980481 , DOI 10.1021/bi012153k , Abstract

Related entities 1. Recoverin, : 1 : 12 : 211 entities Detail

Interaction partners 1. Recoverin, : 2 interactors Detail

Experiments performed 6 experiments Detail