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Solution Structure of the RNase H Domain of the HIV-1 Reverse Transcriptase in Presence of Magnesium
Authors
Pari, K., Mueller, G.A., DeRose, E.F., Kirby, T.W., London, R.E.
Assembly
RNase H domain of the HIV-1 reverse transcriptase
Entity
1. HIV-1 RNase H (polymer, Thiol state: not present), 138 monomers, 15260.14 Da Detail

MNELYQLEKE PIVGAETFYV DGAANRETKL GKAGYVTNRG RQKVVTLTDT TNQKTELQAI YLALQDSGLE VNIVTDSQYA LGIIQAQPDQ SESELVNQII EQLIKKEKVY LAWVPAHKGI GGNEQVDKLV SAGIRKVL


2. MG (non-polymer), 24.305 Da
Total weight
15284.444 Da
Max. entity weight
15260.14 Da
Source organism
HIV-1 M:B_HXB2R
Exptl. method
NMR
Refine. method
AUTOMATED STRUCTURE DETERMINATION WITH ARIA
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 94.2 %, Completeness: 90.2 %, Completeness (bb): 90.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All90.2 % (1456 of 1614)90.7 % (758 of 836)90.1 % (558 of 619)88.1 % (140 of 159)
Backbone90.3 % (732 of 811)90.5 % (247 of 273)90.3 % (364 of 403)89.6 % (121 of 135)
Sidechain90.3 % (840 of 930)90.8 % (511 of 563)90.4 % (310 of 343)79.2 % (19 of 24)
Aromatic94.6 % (70 of 74)97.3 % (36 of 37)91.7 % (33 of 36)100.0 % (1 of 1)
Methyl91.0 % (171 of 188)92.6 % (87 of 94)89.4 % (84 of 94)

1. HIV-1 RNase H

MNELYQLEKE PIVGAETFYV DGAANRETKL GKAGYVTNRG RQKVVTLTDT TNQKTELQAI YLALQDSGLE VNIVTDSQYA LGIIQAQPDQ SESELVNQII EQLIKKEKVY LAWVPAHKGI GGNEQVDKLV SAGIRKVL

Sample

Temperature 298 (±0.25) K, pH 6.8 (±0.01)


#NameIsotope labelingTypeConcentration
1HIV-1 RNase H[U-90% 13C; U-90% 15N]1.1 mM
2magnesium80 mM

LACS Plot; CA
Referencing offset: -0.04 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.04 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.07 ppm, Outliers: 3 Detail
LACS Plot; CO
Referencing offset: 0.15 ppm, Outliers: 3 Detail
Protein Blocks Logo
Calculated from 7 models in PDB: 1O1W, Strand ID: A Detail


Release date
2003-02-24
Citation
Solution structure of the RNase H domain of the HIV-1 reverse transcriptase in the presence of magnesium
Pari, K., Mueller, G.A., DeRose, E.F., Kirby, T.W., London, R.E.
Biochemistry (2003), 42, 639-650, PubMed 12534276 , DOI 10.1021/bi0204894 ,
Related entities 1. HIV-1 RNase H, : 1 : 1 : 94 : 98 entities Detail
Interaction partners 1. HIV-1 RNase H, : 5 interactors Detail
Experiments performed 10 experiments Detail
Keywords HIV-1, RNase H domain, reverse transcriptase, solution structure, 3D NMR, metal binding