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Found 1 Document (2.384 seconds)

BMRB: 5365

2A3S

1H, 15N, 13C resonance assignments for the DNA-binding domain of myocyte nuclear factor

Authors

Chuang, W.

Assembly

myocyte nuclear factor

Entity

1. myocyte nuclear factor (polymer, Thiol state: not present), 114 monomers, 13017.50 Da Detail

ASMTGGQQMG RGSESKPPYS YAQLIVQAIS SAQDRQLTLS GIYAHITKHY PYYRTADKGW QNSIRHNLSL NRYFIKVPRS QEEPGKGSFW RIDPASEAKL VEQAFRKRRQ RGVS

Formula weight

13017.5 Da

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

Hybrid distance geometry-simulated annealing method

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.6 %, Completeness: 64.6 %, Completeness (bb): 70.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	64.6 % (878 of 1359)	81.4 % (585 of 719)	39.1 % (202 of 517)	74.0 % (91 of 123)
Backbone	70.8 % (476 of 672)	83.5 % (193 of 231)	58.0 % (193 of 333)	83.3 % (90 of 108)
Sidechain	62.0 % (491 of 792)	80.3 % (392 of 488)	33.9 % (98 of 289)	6.7 % (1 of 15)
Aromatic	41.8 % (51 of 122)	83.6 % (51 of 61)	0.0 % (0 of 59)	0.0 % (0 of 2)
Methyl	50.0 % (47 of 94)	80.9 % (38 of 47)	19.1 % (9 of 47)

1. myocyte nuclear factor

ASMTGGQQMG RGSESKPPYS YAQLIVQAIS SAQDRQLTLS GIYAHITKHY PYYRTADKGW QNSIRHNLSL NRYFIKVPRS QEEPGKGSFW RIDPASEAKL VEQAFRKRRQ RGVS

Show sample condition

Sample

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	myocyte nuclear factor	[U-15N; U-13C]	3 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 2.08 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 2.08 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.06 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2A3S, Strand ID: A Detail

Release date

2002-11-03

Citation

1H, 15N and 13C resonance assignments for the DNA-binding domain of myocyte nuclear factor (Foxk1)
Chuang, W., Yeh, I., Hsieh, Y., Liu, P., Chen, S., Jeng, W.
J. Biomol. NMR (2002), 24, 75-76, PubMed 12449423 , DOI: , Abstract

Related entities 1. myocyte nuclear factor, : 1 : 4 : 139 entities Detail

Interaction partners 1. myocyte nuclear factor, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 13 experiments Detail

1 1H-15N NOESY-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	myocyte nuclear factor	[U-15N; U-13C]	3 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
4	myocyte nuclear factor	[U-15N]	3 mM
5	phosphate buffer		25 mM
6	NaCl		100 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
7	myocyte nuclear factor	[15N]-Ala	1 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM

Sample #4

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Concentration
10	myocyte nuclear factor	3 mM
11	phosphate buffer	25 mM
12	NaCl	100 mM

2 1H-15N TOCSY-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	myocyte nuclear factor	[U-15N; U-13C]	3 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
4	myocyte nuclear factor	[U-15N]	3 mM
5	phosphate buffer		25 mM
6	NaCl		100 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
7	myocyte nuclear factor	[15N]-Ala	1 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM

Sample #4

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Concentration
10	myocyte nuclear factor	3 mM
11	phosphate buffer	25 mM
12	NaCl	100 mM

3 HNCA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	myocyte nuclear factor	[U-15N; U-13C]	3 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
4	myocyte nuclear factor	[U-15N]	3 mM
5	phosphate buffer		25 mM
6	NaCl		100 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
7	myocyte nuclear factor	[15N]-Ala	1 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM

Sample #4

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Concentration
10	myocyte nuclear factor	3 mM
11	phosphate buffer	25 mM
12	NaCl	100 mM

4 HN(CO)CA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	myocyte nuclear factor	[U-15N; U-13C]	3 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
4	myocyte nuclear factor	[U-15N]	3 mM
5	phosphate buffer		25 mM
6	NaCl		100 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
7	myocyte nuclear factor	[15N]-Ala	1 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM

Sample #4

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Concentration
10	myocyte nuclear factor	3 mM
11	phosphate buffer	25 mM
12	NaCl	100 mM

5 HNCACB

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	myocyte nuclear factor	[U-15N; U-13C]	3 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
4	myocyte nuclear factor	[U-15N]	3 mM
5	phosphate buffer		25 mM
6	NaCl		100 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
7	myocyte nuclear factor	[15N]-Ala	1 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM

Sample #4

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Concentration
10	myocyte nuclear factor	3 mM
11	phosphate buffer	25 mM
12	NaCl	100 mM

6 CACB(CO)NH

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	myocyte nuclear factor	[U-15N; U-13C]	3 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
4	myocyte nuclear factor	[U-15N]	3 mM
5	phosphate buffer		25 mM
6	NaCl		100 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
7	myocyte nuclear factor	[15N]-Ala	1 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM

Sample #4

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Concentration
10	myocyte nuclear factor	3 mM
11	phosphate buffer	25 mM
12	NaCl	100 mM

7 HNHA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	myocyte nuclear factor	[U-15N; U-13C]	3 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
4	myocyte nuclear factor	[U-15N]	3 mM
5	phosphate buffer		25 mM
6	NaCl		100 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
7	myocyte nuclear factor	[15N]-Ala	1 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM

Sample #4

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Concentration
10	myocyte nuclear factor	3 mM
11	phosphate buffer	25 mM
12	NaCl	100 mM

8 HBHA(CBCA)NH

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	myocyte nuclear factor	[U-15N; U-13C]	3 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
4	myocyte nuclear factor	[U-15N]	3 mM
5	phosphate buffer		25 mM
6	NaCl		100 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
7	myocyte nuclear factor	[15N]-Ala	1 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM

Sample #4

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Concentration
10	myocyte nuclear factor	3 mM
11	phosphate buffer	25 mM
12	NaCl	100 mM

9 HBHA(CBCACO)NH

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	myocyte nuclear factor	[U-15N; U-13C]	3 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
4	myocyte nuclear factor	[U-15N]	3 mM
5	phosphate buffer		25 mM
6	NaCl		100 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
7	myocyte nuclear factor	[15N]-Ala	1 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM

Sample #4

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Concentration
10	myocyte nuclear factor	3 mM
11	phosphate buffer	25 mM
12	NaCl	100 mM

10 HCC(CO)NH-TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	myocyte nuclear factor	[U-15N; U-13C]	3 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
4	myocyte nuclear factor	[U-15N]	3 mM
5	phosphate buffer		25 mM
6	NaCl		100 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
7	myocyte nuclear factor	[15N]-Ala	1 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM

Sample #4

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Concentration
10	myocyte nuclear factor	3 mM
11	phosphate buffer	25 mM
12	NaCl	100 mM

11 HCCH-NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	myocyte nuclear factor	[U-15N; U-13C]	3 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
4	myocyte nuclear factor	[U-15N]	3 mM
5	phosphate buffer		25 mM
6	NaCl		100 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
7	myocyte nuclear factor	[15N]-Ala	1 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM

Sample #4

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Concentration
10	myocyte nuclear factor	3 mM
11	phosphate buffer	25 mM
12	NaCl	100 mM

12 HCCH-TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	myocyte nuclear factor	[U-15N; U-13C]	3 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
4	myocyte nuclear factor	[U-15N]	3 mM
5	phosphate buffer		25 mM
6	NaCl		100 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
7	myocyte nuclear factor	[15N]-Ala	1 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM

Sample #4

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Concentration
10	myocyte nuclear factor	3 mM
11	phosphate buffer	25 mM
12	NaCl	100 mM

13 HCCH-COSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	myocyte nuclear factor	[U-15N; U-13C]	3 mM
2	phosphate buffer		25 mM
3	NaCl		100 mM

Sample #2

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
4	myocyte nuclear factor	[U-15N]	3 mM
5	phosphate buffer		25 mM
6	NaCl		100 mM

Sample #3

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
7	myocyte nuclear factor	[15N]-Ala	1 mM
8	phosphate buffer		25 mM
9	NaCl		100 mM

Sample #4

Temperature 300 (±0.1) K, pH 6.0 (±0.05)

#	Name	Concentration
10	myocyte nuclear factor	3 mM
11	phosphate buffer	25 mM
12	NaCl	100 mM

nullKeywords DNA-binding domain, myocyte nuclear factor, resonance assignment

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Found 1 Document (2.384 seconds)

BMRB: 5365

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. myocyte nuclear factor, : 1 : 4 : 139 entities Detail

Interaction partners 1. myocyte nuclear factor, : 2 interactors Detail

Experiments performed 13 experiments Detail

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