BMRB: 5369

A New Zinc Binding Fold Underlines the Versatility of Zinc Binding Modules in Protein Evolution

Authors

Sharpe, B.K., Matthews, J.M., Kwan, A.H.Y., Newton, A., Gell, D.A., Crossley, M., Mackay, J.P.

Assembly

CREB Binding Protein

Entity

1. CREB Binding Protein (polymer, Thiol state: all other bound), 27 monomers, 3065.598 Da Detail

EVRACSLPHC RTMKNVLNHM THCQAGK

2. ZN (non-polymer), 65.409 Da

Total weight

3131.0068 Da

Max. entity weight

3065.598 Da

Entity Connection

na 4 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	2:ZN1:ZN	1:CYS5:SG
2	na	sing	2:ZN1:ZN	1:CYS10:SG
3	na	sing	2:ZN1:ZN	1:HIS19:ND1
4	na	sing	2:ZN1:ZN	1:CYS23:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.0 %, Completeness (bb): 94.4 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	90.0 % (144 of 160)	90.0 % (144 of 160)
Backbone	94.4 % (51 of 54)	94.4 % (51 of 54)
Sidechain	87.7 % (93 of 106)	87.7 % (93 of 106)
Aromatic	100.0 % (6 of 6)	100.0 % (6 of 6)
Methyl	100.0 % (12 of 12)	100.0 % (12 of 12)

1. CREB Binding Protein

EVRACSLPHC RTMKNVLNHM THCQAGK

Show sample condition

Sample

Pressure 1 atm, Temperature 275 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	CREB Binding Protein			0.4 mM
2	ZINC (II) ION			0.6 mM
3	TCEP			0.6 mM

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 1LIQ, Strand ID: A Detail

---

Coupling constant

10 J values in 1 lists
Pressure 1 atm, Temperature 275 (±1) K, pH 6.5 (±0.2) Detail

---

Pressure 1 atm, Temperature 275 (±1) K, pH 6.5 (±0.2)

List #1 J_values_set_1

Release date

2002-05-29

Citation

A new zinc binding fold underlines the versatility of zinc binding modules in protein evolution
Sharpe, B.K., Matthews, J.M., Kwan, A.H.Y., Newton, A., Gell, D.A., Crossley, M., Mackay, J.P.
Structure (2002), 10, 639-648, PubMed 12015147 , DOI: , Abstract

Related entities 1. CREB Binding Protein, : 1 : 11 : 3 : 1 : 22 entities Detail

Interaction partners 1. CREB Binding Protein, : 78 interactors Detail

Experiments performed 4 experiments Detail