BMRB: 5376

C-terminal peptide of alpha-subunit of transducin

Authors

Koenig, B.W., Kontaxis, G., Mitchell, D.C., Louis, J.M., Litman, B.J., Bax, A.

Assembly

complex of transducin peptide and rhodopsin

Entity

1. Gt-alpha(340-350) (polymer, Thiol state: not present), 11 monomers, 1291.453 Da Detail

IRENLKDSGL F

2. rhodopsin (polymer, Thiol state: not reported), 348 monomers, 39007.26 Da Detail

MNGTEGPNFY VPFSNKTGVV RSPFEAPQYY LAEPWQFSML AAYMFLLIML GFPINFLTLY VTVQHKKLRT PLNYILLNLA VADLFMVFGG FTTTLYTSLH GYFVFGPTGC NLEGFFATLG GEIALWSLVV LAIERYVVVC KPMSNFRFGE NHAIMGVAFT WVMALACAAP PLVGWSRYIP EGMQCSCGID YYTPHEETNN ESFVIYMFVV HFIIPLIVIF FCYGQLVFTV KEAAAQQQES ATTQKAEKEV TRMVIIMVIA FLICWLPYAG VAFYIFTHQG SDFGPIFMTI PAFFAKTSAV YNPVIYIMMN KQFRNCMVTT LCCGKNPLGD DEASTTVSKT ETSQVAPA

Total weight

40298.715 Da

Max. entity weight

39007.26 Da

Source organism

Bos taurus

Exptl. method

NMR

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 5.8 %, Completeness: 3.8 %, Completeness (bb): 4.1 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	3.8 % (164 of 4279)	4.3 % (93 of 2183)	3.1 % (53 of 1724)	4.8 % (18 of 372)
Backbone	4.1 % (86 of 2114)	4.6 % (33 of 722)	3.4 % (36 of 1053)	5.0 % (17 of 339)
Sidechain	3.8 % (95 of 2500)	4.1 % (60 of 1461)	3.4 % (34 of 1006)	3.0 % (1 of 33)
Aromatic	0.9 % (5 of 548)	1.8 % (5 of 274)	0.0 % (0 of 269)	0.0 % (0 of 5)
Methyl	4.2 % (19 of 448)	4.9 % (11 of 224)	3.6 % (8 of 224)

1. Gt-alpha(340-350)

IRENLKDSGL F

2. rhodopsin

MNGTEGPNFY VPFSNKTGVV RSPFEAPQYY LAEPWQFSML AAYMFLLIML GFPINFLTLY VTVQHKKLRT PLNYILLNLA VADLFMVFGG FTTTLYTSLH GYFVFGPTGC NLEGFFATLG GEIALWSLVV LAIERYVVVC KPMSNFRFGE NHAIMGVAFT WVMALACAAP PLVGWSRYIP EGMQCSCGID YYTPHEETNN ESFVIYMFVV HFIIPLIVIF FCYGQLVFTV KEAAAQQQES ATTQKAEKEV TRMVIIMVIA FLICWLPYAG VAFYIFTHQG SDFGPIFMTI PAFFAKTSAV YNPVIYIMMN KQFRNCMVTT LCCGKNPLGD DEASTTVSKT ETSQVAPA

Show sample condition

Sample #1

Temperature 283 (±0.5) K, pH 6.6 (±0.2), Details sample contains individual, intact rhodopsin rich disk membranes isolated from the rod outer segment of bovine retina; S2 peptide is in fast exchange between a free form in solution and a rhodopsin bound form

#	Name	Isotope labeling	Type	Concentration
1	Gt-alpha(340-350)	[U-15N; U-13C]		2.6 mM
2	rhodopsin			0.063 mM

Sample #2

Temperature 283 (±0.5) K, pH 6.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Gt-alpha(340-350)			2.6 mM

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 1LVZ, Strand ID: A Detail

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Release date

2003-01-05

Citation

Structure and orientation of a G protein fragment in the receptor bound state from residual dipolar couplings
Koenig, B.W., Kontaxis, G., Mitchell, D.C., Louis, J.M., Litman, B.J., Bax, A.
J. Mol. Biol. (2002), 322, 441-461, PubMed 12217702 , DOI 10.1016/s0022-2836(02)00745-3 , Abstract

Related entities 1. Gt-alpha(340-350), : 1 : 8 entities Detail

Related entities 2. rhodopsin, : 2 : 84 entities Detail

Experiments performed 4 experiments Detail