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C-terminal peptide of alpha-subunit of transducin
Authors
Koenig, B.W., Kontaxis, G., Mitchell, D.C., Louis, J.M., Litman, B.J., Bax, A.
Assembly
complex of transducin peptide and rhodopsin
Entity
1. Gt-alpha(340-350) (polymer, Thiol state: not present), 11 monomers, 1291.453 Da Detail

IRENLKDSGL F


2. rhodopsin (polymer, Thiol state: not reported), 348 monomers, 39007.26 Da Detail

MNGTEGPNFY VPFSNKTGVV RSPFEAPQYY LAEPWQFSML AAYMFLLIML GFPINFLTLY VTVQHKKLRT PLNYILLNLA VADLFMVFGG FTTTLYTSLH GYFVFGPTGC NLEGFFATLG GEIALWSLVV LAIERYVVVC KPMSNFRFGE NHAIMGVAFT WVMALACAAP PLVGWSRYIP EGMQCSCGID YYTPHEETNN ESFVIYMFVV HFIIPLIVIF FCYGQLVFTV KEAAAQQQES ATTQKAEKEV TRMVIIMVIA FLICWLPYAG VAFYIFTHQG SDFGPIFMTI PAFFAKTSAV YNPVIYIMMN KQFRNCMVTT LCCGKNPLGD DEASTTVSKT ETSQVAPA


Total weight
40298.715 Da
Max. entity weight
39007.26 Da
Source organism
Bos taurus
Exptl. method
NMR
Refine. method
simulated annealing, molecular dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 6.1 %, Completeness: 4.3 %, Completeness (bb): 4.2 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All 4.3 % (185 of 4287) 4.9 % (108 of 2183) 3.5 % (60 of 1724) 4.5 % (17 of 380)
Backbone 4.2 % (88 of 2090) 4.7 % (33 of 698) 3.7 % (39 of 1053) 4.7 % (16 of 339)
Sidechain 4.5 % (113 of 2532) 5.1 % (76 of 1485) 3.6 % (36 of 1006) 2.4 % (1 of 41)
Aromatic 1.5 % (8 of 548) 2.9 % (8 of 274) 0.0 % (0 of 269) 0.0 % (0 of 5)
Methyl 4.5 % (20 of 448) 5.4 % (12 of 224) 3.6 % (8 of 224)

1. Gt-alpha(340-350)

IRENLKDSGL F

2. rhodopsin

MNGTEGPNFY VPFSNKTGVV RSPFEAPQYY LAEPWQFSML AAYMFLLIML GFPINFLTLY VTVQHKKLRT PLNYILLNLA VADLFMVFGG FTTTLYTSLH GYFVFGPTGC NLEGFFATLG GEIALWSLVV LAIERYVVVC KPMSNFRFGE NHAIMGVAFT WVMALACAAP PLVGWSRYIP EGMQCSCGID YYTPHEETNN ESFVIYMFVV HFIIPLIVIF FCYGQLVFTV KEAAAQQQES ATTQKAEKEV TRMVIIMVIA FLICWLPYAG VAFYIFTHQG SDFGPIFMTI PAFFAKTSAV YNPVIYIMMN KQFRNCMVTT LCCGKNPLGD DEASTTVSKT ETSQVAPA

Sample #1

Temperature 283 (±0.5) K, pH 6.6 (±0.2), Details sample contains individual, intact rhodopsin rich disk membranes isolated from the rod outer segment of bovine retina; S2 peptide is in fast exchange between a free form in solution and a rhodopsin bound form


#NameIsotope labelingTypeConcentration
1Gt-alpha(340-350)[U-15N; U-13C]2.6 mM
2rhodopsin0.063 mM
Sample #2

Temperature 283 (±0.5) K, pH 6.6 (±0.2)


#NameIsotope labelingTypeConcentration
3Gt-alpha(340-350)2.6 mM

Protein Blocks Logo
Calculated from 20 models in PDB: 1LVZ, Strand ID: A Detail


Release date
2003-01-05
Citation
Structure and orientation of a G protein fragment in the receptor bound state from residual dipolar couplings
Koenig, B.W., Kontaxis, G., Mitchell, D.C., Louis, J.M., Litman, B.J., Bax, A.
J. Mol. Biol. (2002), 322, 441-461, PubMed 12217702 ,
Related entities 1. Gt-alpha(340-350), : 1 : 8 entities Detail
Related entities 2. rhodopsin, : 27 : 15 : 125 entities Detail
Interaction partners 2. rhodopsin, : 6 interactors Detail
Experiments performed 4 experiments Detail
Keywords NMR, membrane protein, rhodopsin, transducin, transferred dipolar couplings