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BMRB: 5396

1LUD

NMR-based solution structure of the complex of Lactobacillus casei dihydrofolate reductase with trimethoprim and NADPH

Authors

Polshakov, V.I., Smirnov, E.G., Birdsall, B., Kelly, G., Feeney, J.

Assembly

ternary complex DHFR-trimethoprim-NADPH

Entity

1. Dihydrofolate reductase (polymer, Thiol state: not present), 162 monomers, 18307.38 Da Detail

TAFLWAQDRD GLIGKDGHLP WHLPDDLHYF RAQTVGKIMV VGRRTYESFP KRPLPERTNV VLTHQEDYQA QGAVVVHDVA AVFAYAKQHP DQELVIAGGA QIFTAFKDDV DTLLVTRLAG SFEGDTKMIP LNWDDFTKVS SRTVEDTNPA LTHTYEVWQK KA

2. NAP (non-polymer), 743.405 Da
3. TOP (non-polymer), 290.318 Da

Total weight

19341.104 Da

Max. entity weight

18307.38 Da

Source organism

Lacticaseibacillus casei

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 92.4 %, Completeness (bb): 99.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	92.4 % (1054 of 1141)	91.5 % (888 of 971)	97.6 % (166 of 170)
Backbone	99.8 % (479 of 480)	99.7 % (325 of 326)	100.0 % (154 of 154)
Sidechain	87.0 % (575 of 661)	87.3 % (563 of 645)	75.0 % (12 of 16)
Aromatic	69.6 % (71 of 102)	72.4 % (71 of 98)	0.0 % (0 of 4)
Methyl	100.0 % (97 of 97)	100.0 % (97 of 97)

1. Dihydrofolate reductase

TAFLWAQDRD GLIGKDGHLP WHLPDDLHYF RAQTVGKIMV VGRRTYESFP KRPLPERTNV VLTHQEDYQA QGAVVVHDVA AVFAYAKQHP DQELVIAGGA QIFTAFKDDV DTLLVTRLAG SFEGDTKMIP LNWDDFTKVS SRTVEDTNPA LTHTYEVWQK KA

Show sample condition

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Dihydrofolate reductase	[U-95% 15N]	1.5 ~ 4.0 mM
2	TRIMETHOPRIM	[U-95% 15N]	1.5 ~ 4.0 mM
3	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	1.5 ~ 4.0 mM
4	potassium phosphate		25 mM
5	KCl		50 mM

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Concentration
6	Dihydrofolate reductase	2.0 ~ 4.0 mM
7	TRIMETHOPRIM	2.0 ~ 4.0 mM
8	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	2.0 ~ 4.0 mM
9	potassium phosphate	25 mM
10	KCl	50 mM

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
11	Dihydrofolate reductase	[U-95% 15N]	0.5 mM
12	TRIMETHOPRIM	[U-95% 15N]	0.5 mM
13	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	0.5 mM
14	DMPC/DHPC (3/1)		5 %
15	H2O/D2O (90/10)		95 %

Chem. Shift Complete2

Sequence coverage: 24.1 %, Completeness: 54.5 %, Completeness (bb): 59.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	54.5 % (1243 of 2282)	53.9 % (1047 of 1942)	57.6 % (196 of 340)
Backbone	59.6 % (572 of 960)	59.8 % (390 of 652)	59.1 % (182 of 308)
Sidechain	50.4 % (666 of 1322)	50.6 % (653 of 1290)	40.6 % (13 of 32)
Aromatic	35.8 % (73 of 204)	37.2 % (73 of 196)	0.0 % (0 of 8)
Methyl	58.8 % (114 of 194)	58.8 % (114 of 194)

1. Dihydrofolate reductase

TAFLWAQDRD GLIGKDGHLP WHLPDDLHYF RAQTVGKIMV VGRRTYESFP KRPLPERTNV VLTHQEDYQA QGAVVVHDVA AVFAYAKQHP DQELVIAGGA QIFTAFKDDV DTLLVTRLAG SFEGDTKMIP LNWDDFTKVS SRTVEDTNPA LTHTYEVWQK KA

Show sample condition

Sample

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Dihydrofolate reductase	[U-95% 15N]	1.5 ~ 4.0 mM
2	TRIMETHOPRIM	[U-95% 15N]	1.5 ~ 4.0 mM
3	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	1.5 ~ 4.0 mM
4	potassium phosphate		25 mM
5	KCl		50 mM

Chem. Shift Complete3

Sequence coverage: 14.2 %, Completeness: 39.6 %, Completeness (bb): 43.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	39.6 % (1354 of 3423)	39.1 % (1140 of 2913)	42.0 % (214 of 510)
Backbone	43.3 % (623 of 1440)	43.4 % (424 of 978)	43.1 % (199 of 462)
Sidechain	36.7 % (728 of 1983)	36.9 % (714 of 1935)	29.2 % (14 of 48)
Aromatic	28.4 % (87 of 306)	29.6 % (87 of 294)	0.0 % (0 of 12)
Methyl	42.6 % (124 of 291)	42.6 % (124 of 291)

1. Dihydrofolate reductase

TAFLWAQDRD GLIGKDGHLP WHLPDDLHYF RAQTVGKIMV VGRRTYESFP KRPLPERTNV VLTHQEDYQA QGAVVVHDVA AVFAYAKQHP DQELVIAGGA QIFTAFKDDV DTLLVTRLAG SFEGDTKMIP LNWDDFTKVS SRTVEDTNPA LTHTYEVWQK KA

Show sample condition

Sample

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Dihydrofolate reductase	[U-95% 15N]	1.5 ~ 4.0 mM
2	TRIMETHOPRIM	[U-95% 15N]	1.5 ~ 4.0 mM
3	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	1.5 ~ 4.0 mM
4	potassium phosphate		25 mM
5	KCl		50 mM

Protein Blocks Logo

Calculated from 24 models in PDB: 1LUD, Strand ID: A Detail

Release date

2002-11-03

Citation

NMR-based solution structure of the complex of Lactobacillus casei dihydrofolate reductase with trimethoprim and NADPH
Polshakov, V.I., Smirnov, E.G., Birdsall, B., Kelly, G., Feeney, J.
J. Biomol. NMR (2002), 24, 67-70, PubMed 12449420 , Abstract

Related entities 1. Dihydrofolate reductase, : 1 : 9 : 137 entities Detail

Experiments performed 10 experiments Detail

1 DQF-COSY

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian UNITYPlus (500 MHz)

#3 Model Varian INOVA (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Dihydrofolate reductase	[U-95% 15N]	1.5 ~ 4.0 mM
2	TRIMETHOPRIM	[U-95% 15N]	1.5 ~ 4.0 mM
3	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	1.5 ~ 4.0 mM
4	potassium phosphate		25 mM
5	KCl		50 mM

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Concentration
6	Dihydrofolate reductase	2.0 ~ 4.0 mM
7	TRIMETHOPRIM	2.0 ~ 4.0 mM
8	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	2.0 ~ 4.0 mM
9	potassium phosphate	25 mM
10	KCl	50 mM

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
11	Dihydrofolate reductase	[U-95% 15N]	0.5 mM
12	TRIMETHOPRIM	[U-95% 15N]	0.5 mM
13	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	0.5 mM
14	DMPC/DHPC (3/1)		5 %
15	H2O/D2O (90/10)		95 %

2 TOCSY

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian UNITYPlus (500 MHz)

#3 Model Varian INOVA (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Dihydrofolate reductase	[U-95% 15N]	1.5 ~ 4.0 mM
2	TRIMETHOPRIM	[U-95% 15N]	1.5 ~ 4.0 mM
3	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	1.5 ~ 4.0 mM
4	potassium phosphate		25 mM
5	KCl		50 mM

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Concentration
6	Dihydrofolate reductase	2.0 ~ 4.0 mM
7	TRIMETHOPRIM	2.0 ~ 4.0 mM
8	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	2.0 ~ 4.0 mM
9	potassium phosphate	25 mM
10	KCl	50 mM

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
11	Dihydrofolate reductase	[U-95% 15N]	0.5 mM
12	TRIMETHOPRIM	[U-95% 15N]	0.5 mM
13	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	0.5 mM
14	DMPC/DHPC (3/1)		5 %
15	H2O/D2O (90/10)		95 %

3 NOESY

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian UNITYPlus (500 MHz)

#3 Model Varian INOVA (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Dihydrofolate reductase	[U-95% 15N]	1.5 ~ 4.0 mM
2	TRIMETHOPRIM	[U-95% 15N]	1.5 ~ 4.0 mM
3	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	1.5 ~ 4.0 mM
4	potassium phosphate		25 mM
5	KCl		50 mM

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Concentration
6	Dihydrofolate reductase	2.0 ~ 4.0 mM
7	TRIMETHOPRIM	2.0 ~ 4.0 mM
8	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	2.0 ~ 4.0 mM
9	potassium phosphate	25 mM
10	KCl	50 mM

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
11	Dihydrofolate reductase	[U-95% 15N]	0.5 mM
12	TRIMETHOPRIM	[U-95% 15N]	0.5 mM
13	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	0.5 mM
14	DMPC/DHPC (3/1)		5 %
15	H2O/D2O (90/10)		95 %

4 1H-15N HSQC

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian UNITYPlus (500 MHz)

#3 Model Varian INOVA (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Dihydrofolate reductase	[U-95% 15N]	1.5 ~ 4.0 mM
2	TRIMETHOPRIM	[U-95% 15N]	1.5 ~ 4.0 mM
3	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	1.5 ~ 4.0 mM
4	potassium phosphate		25 mM
5	KCl		50 mM

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Concentration
6	Dihydrofolate reductase	2.0 ~ 4.0 mM
7	TRIMETHOPRIM	2.0 ~ 4.0 mM
8	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	2.0 ~ 4.0 mM
9	potassium phosphate	25 mM
10	KCl	50 mM

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
11	Dihydrofolate reductase	[U-95% 15N]	0.5 mM
12	TRIMETHOPRIM	[U-95% 15N]	0.5 mM
13	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	0.5 mM
14	DMPC/DHPC (3/1)		5 %
15	H2O/D2O (90/10)		95 %

5 1H-15N NOESY-HSQC

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian UNITYPlus (500 MHz)

#3 Model Varian INOVA (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Dihydrofolate reductase	[U-95% 15N]	1.5 ~ 4.0 mM
2	TRIMETHOPRIM	[U-95% 15N]	1.5 ~ 4.0 mM
3	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	1.5 ~ 4.0 mM
4	potassium phosphate		25 mM
5	KCl		50 mM

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Concentration
6	Dihydrofolate reductase	2.0 ~ 4.0 mM
7	TRIMETHOPRIM	2.0 ~ 4.0 mM
8	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	2.0 ~ 4.0 mM
9	potassium phosphate	25 mM
10	KCl	50 mM

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
11	Dihydrofolate reductase	[U-95% 15N]	0.5 mM
12	TRIMETHOPRIM	[U-95% 15N]	0.5 mM
13	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	0.5 mM
14	DMPC/DHPC (3/1)		5 %
15	H2O/D2O (90/10)		95 %

6 1H-15N TOCSY-HSQC

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian UNITYPlus (500 MHz)

#3 Model Varian INOVA (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Dihydrofolate reductase	[U-95% 15N]	1.5 ~ 4.0 mM
2	TRIMETHOPRIM	[U-95% 15N]	1.5 ~ 4.0 mM
3	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	1.5 ~ 4.0 mM
4	potassium phosphate		25 mM
5	KCl		50 mM

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Concentration
6	Dihydrofolate reductase	2.0 ~ 4.0 mM
7	TRIMETHOPRIM	2.0 ~ 4.0 mM
8	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	2.0 ~ 4.0 mM
9	potassium phosphate	25 mM
10	KCl	50 mM

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
11	Dihydrofolate reductase	[U-95% 15N]	0.5 mM
12	TRIMETHOPRIM	[U-95% 15N]	0.5 mM
13	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	0.5 mM
14	DMPC/DHPC (3/1)		5 %
15	H2O/D2O (90/10)		95 %

7 HNHA

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian UNITYPlus (500 MHz)

#3 Model Varian INOVA (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Dihydrofolate reductase	[U-95% 15N]	1.5 ~ 4.0 mM
2	TRIMETHOPRIM	[U-95% 15N]	1.5 ~ 4.0 mM
3	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	1.5 ~ 4.0 mM
4	potassium phosphate		25 mM
5	KCl		50 mM

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Concentration
6	Dihydrofolate reductase	2.0 ~ 4.0 mM
7	TRIMETHOPRIM	2.0 ~ 4.0 mM
8	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	2.0 ~ 4.0 mM
9	potassium phosphate	25 mM
10	KCl	50 mM

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
11	Dihydrofolate reductase	[U-95% 15N]	0.5 mM
12	TRIMETHOPRIM	[U-95% 15N]	0.5 mM
13	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	0.5 mM
14	DMPC/DHPC (3/1)		5 %
15	H2O/D2O (90/10)		95 %

8 HNHB

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian UNITYPlus (500 MHz)

#3 Model Varian INOVA (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Dihydrofolate reductase	[U-95% 15N]	1.5 ~ 4.0 mM
2	TRIMETHOPRIM	[U-95% 15N]	1.5 ~ 4.0 mM
3	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	1.5 ~ 4.0 mM
4	potassium phosphate		25 mM
5	KCl		50 mM

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Concentration
6	Dihydrofolate reductase	2.0 ~ 4.0 mM
7	TRIMETHOPRIM	2.0 ~ 4.0 mM
8	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	2.0 ~ 4.0 mM
9	potassium phosphate	25 mM
10	KCl	50 mM

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
11	Dihydrofolate reductase	[U-95% 15N]	0.5 mM
12	TRIMETHOPRIM	[U-95% 15N]	0.5 mM
13	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	0.5 mM
14	DMPC/DHPC (3/1)		5 %
15	H2O/D2O (90/10)		95 %

9 15N-rejected NOESY

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian UNITYPlus (500 MHz)

#3 Model Varian INOVA (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Dihydrofolate reductase	[U-95% 15N]	1.5 ~ 4.0 mM
2	TRIMETHOPRIM	[U-95% 15N]	1.5 ~ 4.0 mM
3	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	1.5 ~ 4.0 mM
4	potassium phosphate		25 mM
5	KCl		50 mM

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Concentration
6	Dihydrofolate reductase	2.0 ~ 4.0 mM
7	TRIMETHOPRIM	2.0 ~ 4.0 mM
8	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	2.0 ~ 4.0 mM
9	potassium phosphate	25 mM
10	KCl	50 mM

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
11	Dihydrofolate reductase	[U-95% 15N]	0.5 mM
12	TRIMETHOPRIM	[U-95% 15N]	0.5 mM
13	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	0.5 mM
14	DMPC/DHPC (3/1)		5 %
15	H2O/D2O (90/10)		95 %

10 J-modulated 1H-15N HSQC

Instrument

#1 Model Varian UNITY (600 MHz)

#2 Model Varian UNITYPlus (500 MHz)

#3 Model Varian INOVA (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	Dihydrofolate reductase	[U-95% 15N]	1.5 ~ 4.0 mM
2	TRIMETHOPRIM	[U-95% 15N]	1.5 ~ 4.0 mM
3	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	1.5 ~ 4.0 mM
4	potassium phosphate		25 mM
5	KCl		50 mM

Sample #2

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Concentration
6	Dihydrofolate reductase	2.0 ~ 4.0 mM
7	TRIMETHOPRIM	2.0 ~ 4.0 mM
8	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	2.0 ~ 4.0 mM
9	potassium phosphate	25 mM
10	KCl	50 mM

Sample #3

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
11	Dihydrofolate reductase	[U-95% 15N]	0.5 mM
12	TRIMETHOPRIM	[U-95% 15N]	0.5 mM
13	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE	[U-95% 15N]	0.5 mM
14	DMPC/DHPC (3/1)		5 %
15	H2O/D2O (90/10)		95 %

nullKeywords DHFR, dihydrofolate reductase, trimethoprim, protein-ligand interactions, cooperative binding

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Found 1 Document (2.475 seconds)

BMRB: 5396

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Related entities 1. Dihydrofolate reductase, : 1 : 9 : 137 entities Detail

Experiments performed 10 experiments Detail

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