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Structural model for an alkaline form of ferricytochrome c
Authors
Assfalg, M., Bertini, I., Dolfi, A., Turano, P., Mauk, A.G., Rosell, F.I., Gray, H.B.
Assembly
Cytochrome c, iso-1
Entity
1. Cytochrome c, iso-1 (polymer, Thiol state: not reported), 95 monomers, 10656.99 Da Detail

LFKTRCLQCH TVEKGGPHKV GPNLHGIFGR HSGQAEGYSY TDANIKKNVL WDENNMSEYL TNPAKYIPGT AMAFGGLKKE KDRNDLITYL KKATE


2. HEM (non-polymer), 616.487 Da
Total weight
11273.478 Da
Max. entity weight
10656.99 Da
Entity Connection
metal coordination 1 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1metal coordinationsing1:LYS65:NZ2:HEM1:FE

Source organism
Saccharomyces cerevisiae
Exptl. method
NMR
Refine. method
simulated annealing in torsion angle space
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 85.3 %, Completeness: 62.0 %, Completeness (bb): 77.3 % Detail

Polymer type: polypeptide(L)

Total1H15N
All62.0 % (427 of 689)60.3 % (353 of 585)71.2 % (74 of 104)
Backbone77.3 % (214 of 277)77.4 % (144 of 186)76.9 % (70 of 91)
Sidechain51.7 % (213 of 412)52.4 % (209 of 399)30.8 % (4 of 13)
Aromatic48.0 % (24 of 50)46.9 % (23 of 49)100.0 % (1 of 1)
Methyl74.4 % (32 of 43)74.4 % (32 of 43)

1. Cytochrome c, iso-1

LFKTRCLQCH TVEKGGPHKV GPNLHGIFGR HSGQAEGYSY TDANIKKNVL WDENNMSEYL TNPAKYIPGT AMAFGGLKKE KDRNDLITYL KKATE

Sample

Pressure 1 atm, Temperature 298 K, pH 11.1


#NameIsotope labelingTypeConcentration
1Cytochrome c, iso-12 mM
2PROTOPORPHYRIN IX CONTAINING FE0.0 ~ 0.0 mM
3phosphate buffer50 mM
4H2O90 %
5D2O10 %

Protein Blocks Logo
Calculated from 1 models in PDB: 1LMS, Strand ID: A Detail


Release date
2003-04-22
Citation
Structural model for an alkaline form of ferricytochrome C
Assfalg, M., Bertini, I., Dolfi, A., Turano, P., Mauk, A.G., Rosell, F.I., Gray, H.B.
J. Am. Chem. Soc. (2003), 125, 2913-2922, PubMed 12617658 , DOI 10.1021/ja027180s ,
Related entities 1. Cytochrome c, iso-1, : 1 : 53 : 146 entities Detail
Interaction partners 1. Cytochrome c, iso-1, : 4 interactors Detail
Experiments performed 3 experiments Detail
Keywords alkaline transition, cytochrome c; NMR structure