BMRB: 5466

Structural model for an alkaline form of ferricytochrome c

Authors

Assfalg, M., Bertini, I., Dolfi, A., Turano, P., Mauk, A.G., Rosell, F.I., Gray, H.B.

Assembly

Cytochrome c, iso-1

Entity

1. Cytochrome c, iso-1 (polymer, Thiol state: not reported), 95 monomers, 10656.99 Da Detail

LFKTRCLQCH TVEKGGPHKV GPNLHGIFGR HSGQAEGYSY TDANIKKNVL WDENNMSEYL TNPAKYIPGT AMAFGGLKKE KDRNDLITYL KKATE

2. HEM (non-polymer), 616.487 Da

Total weight

11273.478 Da

Max. entity weight

10656.99 Da

Entity Connection

metal coordination 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:LYS65:NZ	2:HEM1:FE

Source organism

Saccharomyces cerevisiae

Exptl. method

NMR

Refine. method

simulated annealing in torsion angle space

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 85.3 %, Completeness: 62.2 %, Completeness (bb): 76.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	62.2 % (427 of 686)	60.7 % (355 of 585)	71.3 % (72 of 101)
Backbone	76.3 % (219 of 287)	77.6 % (152 of 196)	73.6 % (67 of 91)
Sidechain	52.1 % (208 of 399)	52.2 % (203 of 389)	50.0 % (5 of 10)
Aromatic	48.0 % (24 of 50)	46.9 % (23 of 49)	100.0 % (1 of 1)
Methyl	76.7 % (33 of 43)	76.7 % (33 of 43)

1. Cytochrome c, iso-1

LFKTRCLQCH TVEKGGPHKV GPNLHGIFGR HSGQAEGYSY TDANIKKNVL WDENNMSEYL TNPAKYIPGT AMAFGGLKKE KDRNDLITYL KKATE

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 11.1

#	Name	Isotope labeling	Type	Concentration
1	Cytochrome c, iso-1			2 mM
2	PROTOPORPHYRIN IX CONTAINING FE			0.0 ~ 0.0 mM
3	phosphate buffer			50 mM
4	H2O			90 %
5	D2O			10 %

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Protein Blocks Logo

Calculated from 1 models in PDB: 1LMS, Strand ID: A Detail

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Release date

2003-04-22

Citation

Structural model for an alkaline form of ferricytochrome C
Assfalg, M., Bertini, I., Dolfi, A., Turano, P., Mauk, A.G., Rosell, F.I., Gray, H.B.
J. Am. Chem. Soc. (2003), 125, 2913-2922, PubMed 12617658 , DOI 10.1021/ja027180s , Abstract

Related entities 1. Cytochrome c, iso-1, : 1 : 67 : 168 entities Detail

Interaction partners 1. Cytochrome c, iso-1, : 4 interactors Detail

Experiments performed 3 experiments Detail