BMRB: 5469

Inhibitory Specificity Change of Ovomucoid Third Domain of Silver Pheasant upon Introduction of an Engineered Cys14-Cys39 Bond

Authors

Hemmi, H., Kumazaki, T., Yamazaki, T., Kojima, S., Yoshida, T., Kyogoku, Y., Katsu, M., Shinohara, F., Yokosawa, H., Miura, K., Kobayashi, Y.

Assembly

omsvp3 monomer

Entity

1. omsvp3 monomer (polymer, Thiol state: all disulfide bound), 54 monomers, 5854.562 Da Detail

AVSVDCSEYP KPACTMEYRP LCGSDNKTYG NKCNFCNAVV ESNGTLTLSH FGKC

Formula weight

5854.562 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS6:SG	1:CYS36:SG
2	disulfide	sing	1:CYS14:SG	1:CYS33:SG
3	disulfide	sing	1:CYS22:SG	1:CYS54:SG

Source organism

Lophura nycthemera

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 97.7 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	97.7 % (296 of 303)	97.7 % (296 of 303)
Backbone	100.0 % (109 of 109)	100.0 % (109 of 109)
Sidechain	96.4 % (187 of 194)	96.4 % (187 of 194)
Aromatic	91.7 % (22 of 24)	91.7 % (22 of 24)
Methyl	100.0 % (21 of 21)	100.0 % (21 of 21)

1. ovomucoid third domain from silver pheasant

AVSVDCSEYP KPACTMEYRP LCGSDNKTYG NKCNFCNAVV ESNGTLTLSH FGKC

Show sample condition

Sample

Temperature 298 (±1) K, pH 4.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	ovomucoid third domain from silver pheasant			4.0 mM

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Protein Blocks Logo

Calculated from 15 models in PDB: 1IY5, Strand ID: A Detail

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Release date

2002-07-22

Citation

Inhibitory specificity change of the ovomucoid third domain of the silver pheasant upon introduction of an engineered Cys14-Cys39 bond
Hemmi, H., Kumazaki, T., Yamazaki, T., Kojima, S., Yoshida, T., Kyogoku, Y., Katsu, M., Shinohara, F., Yokosawa, H., Miura, K., Kobayashi, Y.
Biochemistry (2003), 42, 2524-2534, PubMed 12614146 , DOI 10.1021/bi026727c , Abstract

Related entities 1. omsvp3 monomer, : 1 : 3 : 14 : 31 : 192 entities Detail

Interaction partners 1. omsvp3 monomer, : 3 interactors Detail

Experiments performed 3 experiments Detail