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Backbone resonance assignment of the 298 amino acid catalytic domain of protein tyrosine phosphatase 1B (PTP1B)
Authors
Meier, S., Li, Y., Koehn, J., Vlattas, I., Wareing, J., Jahnke, W., Wennogle, L.P., Grzesiek, S.
Assembly
protein tyrosine phosphatase 1B
Entity
1. protein tyrosine phosphatase 1B (polymer, Thiol state: all free), 298 monomers, 34672.14 Da Detail

MEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW EQKSRGVVML NRVMEKGSLK CAQYWPQKEE KEMIFEDTNL KLTLISEDIK SYYTVRQLEL ENLTTQETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE SGSLSPEHGP VVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL LEMRKFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHED


2. DADEXLIP-amide,(X=phenylalanine-p-difluoromethyl phosphonate) (polymer, Thiol state: all free), 8 monomers, 34672 Da Detail

DADEXLIP


Total weight
69344.14 Da
Max. entity weight
34672.14 Da
Entity Connection
na 1 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1nasing2:ASP1:na1:CYS215:SG

Source organism
Homo sapiens
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 81.7 %, Completeness: 36.0 %, Completeness (bb): 66.7 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All36.0 % (1328 of 3688)18.6 % (358 of 1924)52.1 % (742 of 1425)67.3 % (228 of 339)
Backbone66.7 % (1194 of 1790)42.4 % (253 of 597)79.2 % (714 of 901)77.7 % (227 of 292)
Sidechain16.2 % (354 of 2189) 7.9 % (105 of 1327)30.4 % (248 of 815) 2.1 % (1 of 47)
Aromatic 0.0 % (0 of 320) 0.0 % (0 of 160) 0.0 % (0 of 154) 0.0 % (0 of 6)
Methyl15.4 % (46 of 298)12.8 % (19 of 149)18.1 % (27 of 149)

1. protein tyrosine phosphatase 1B

MEMEKEFEQI DKSGSWAAIY QDIRHEASDF PCRVAKLPKN KNRNRYRDVS PFDHSRIKLH QEDNDYINAS LIKMEEAQRS YILTQGPLPN TCGHFWEMVW EQKSRGVVML NRVMEKGSLK CAQYWPQKEE KEMIFEDTNL KLTLISEDIK SYYTVRQLEL ENLTTQETRE ILHFHYTTWP DFGVPESPAS FLNFLFKVRE SGSLSPEHGP VVVHCSAGIG RSGTFCLADT CLLLMDKRKD PSSVDIKKVL LEMRKFRMGL IQTADQLRFS YLAVIEGAKF IMGDSSVQDQ WKELSHED

2. DADEXLIP-amide,(X=phenylalanine-p-difluoromethyl phosphonate)

DADEXLIP

Sample

Temperature 293 (±1) K, pH 7.5 (±0.1)


#NameIsotope labelingTypeConcentration
1protein tyrosine phosphatase 1B[U-100% 15N; U-100% 13C]0.5 mM
2DADEXLIP1 mM
3H2O30 %
4D2O70 %

Release date
2002-11-04
Citation 1
Backbone resonance assignment of the 298 amino acid catalytic domain of protein tyrosine phosphatase 1B (PTP1B)
Meier, S., Li, Y., Koehn, J., Vlattas, I., Wareing, J., Jahnke, W., Wennogle, L.P., Grzesiek, S.
J. Biomol. NMR (2002), 24, 165-166, PubMed 12495035 , DOI: ,
Citation 2
Crystal structure of human protein tyrosine phosphatase 1B
Barford, D., Flint, A.J., Tonks, N.K.
Science (1994), 263, 1397-1404, PubMed 8128219 , DOI 10.2210/pdb2hnp/pdb ,
Experiments performed 13 experiments Detail
Keywords obesity, diabetes, heteronuclear NMR