BMRBj - BMREntryMaster

Found 1 Document (1.863 seconds)

BMRB: 5482

1MJD

Assignment of 1H, 13C and 15N resonances of the N-terminal microtubule-binding domain of human doublecortin

Authors

Cierpicki, T., Kim, M., Otlewski, J., Derewenda, Z.S., Bushweller, J.H.

Assembly

DC 45-150

Entity

1. DC 45-150 (polymer, Thiol state: all free), 113 monomers, 12955.35 Da Detail

GAMDPEFALS NEKKAKKVRF YRNGDRYFKG IVYAVSSDRF RSFDALLADL TRSLSDNINL PQGVRYIYTI DGSRKIGSMD ELEEGESYVC SSDNFFKKVE YTKNVNPNWS VNV

Formula weight

12955.35 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

automatic NOESY cross-peaks assignment, torsion angle dynamics, simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.6 %, Completeness (bb): 82.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.6 % (1171 of 1337)	97.9 % (683 of 698)	72.4 % (375 of 518)	93.4 % (113 of 121)
Backbone	82.4 % (554 of 672)	97.8 % (225 of 230)	67.5 % (224 of 332)	95.5 % (105 of 110)
Sidechain	93.8 % (723 of 771)	97.9 % (458 of 468)	88.0 % (257 of 292)	72.7 % (8 of 11)
Aromatic	74.6 % (103 of 138)	95.7 % (66 of 69)	52.9 % (36 of 68)	100.0 % (1 of 1)
Methyl	100.0 % (102 of 102)	100.0 % (51 of 51)	100.0 % (51 of 51)

1. doublecortin

GAMDPEFALS NEKKAKKVRF YRNGDRYFKG IVYAVSSDRF RSFDALLADL TRSLSDNINL PQGVRYIYTI DGSRKIGSMD ELEEGESYVC SSDNFFKKVE YTKNVNPNWS VNV

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	doublecortin	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	doublecortin	[U-15N]		1.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.36 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.36 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.05 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1MJD, Strand ID: A Detail

Coupling constant

94 J values in 1 lists
Field strength (¹H) 500 MHz, Temperature 298 (±1) K, pH 6.0 (±0.2) Detail

Release date

2003-01-13

Citation 1

Assignment of 1H, 13C and 15N resonances of the N-terminal microtubule-binding domain of human doublecortin
Cierpicki, T., Kim, M., Otlewski, J., Derewenda, Z.S., Bushweller, J.H.
J. Biomol. NMR (2003), 25, 81-82, PubMed 12567003 , DOI: , Abstract

Show more 1 citaion

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , Abstract

Hide non-primary 1 citaion

Related entities 1. DC 45-150, : 1 : 15 : 44 entities Detail

Interaction partners 1. DC 45-150, : 17 interactors Detail

More details for 17 interactors identified by 3 citations

Experiments performed 12 experiments Detail

1 1H-15N HSQC

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	doublecortin	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	doublecortin	[U-15N]		1.0 mM

2 1H-15N TOCSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	doublecortin	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	doublecortin	[U-15N]		1.0 mM

3 HNCO

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	doublecortin	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	doublecortin	[U-15N]		1.0 mM

4 HNCACB

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	doublecortin	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	doublecortin	[U-15N]		1.0 mM

5 CBCA(CO)NH

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	doublecortin	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	doublecortin	[U-15N]		1.0 mM

6 CC(CO)NH-TOCSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	doublecortin	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	doublecortin	[U-15N]		1.0 mM

7 HC(CO)NH-TOCSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	doublecortin	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	doublecortin	[U-15N]		1.0 mM

8 HNHA

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	doublecortin	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	doublecortin	[U-15N]		1.0 mM

9 HCCH-TOCSY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	doublecortin	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	doublecortin	[U-15N]		1.0 mM

10 HACAHB

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	doublecortin	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	doublecortin	[U-15N]		1.0 mM

11 15N NOESY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	doublecortin	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	doublecortin	[U-15N]		1.0 mM

12 13C NOESY

Instrument

Model Varian INOVA (500 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	doublecortin	[U-13C; U-15N]		1.0 mM

Sample #2

Temperature 298 (±1) K, pH 6.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
2	doublecortin	[U-15N]		1.0 mM

nullKeywords doublecortin, tubulin

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Found 1 Document (1.863 seconds)

BMRB: 5482

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Chemical shift reference

Related entities 1. DC 45-150, : 1 : 15 : 44 entities Detail

Interaction partners 1. DC 45-150, : 17 interactors Detail

Experiments performed 12 experiments Detail

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