Search

Resonance assignments of the central complement control protein module pair of human decay accelerating factor
Authors
Uhrinova, S.
Assembly
double module
Entity
1. double module (polymer, Thiol state: all disulfide bound), 134 monomers, 15021.87 Da Detail

FRSCEVPTRL NSASLKQPYI TQNYFPVGTV VEYECRPGYR REPSLSPKLT CLQNLKWSTA VEFCKKKSCP NPGEIRNGQI DVPGGILFGA TISFSCNTGY KLFGSTSSFC LISGSSVQWS DPLPECREHH HHHH


Formula weight
15021.87 Da
Source organism
Komagataella pastoris
Exptl. method
NMR
Refine. method
molecular dynamics
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 96.3 %, Completeness: 70.2 %, Completeness (bb): 76.2 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All70.2 % (1100 of 1567)77.4 % (631 of 815)59.3 % (361 of 609)75.5 % (108 of 143)
Backbone76.2 % (588 of 772)92.6 % (238 of 257)62.0 % (243 of 392)87.0 % (107 of 123)
Sidechain67.9 % (624 of 919)70.4 % (393 of 558)67.4 % (230 of 341) 5.0 % (1 of 20)
Aromatic41.1 % (65 of 158)63.3 % (50 of 79)19.5 % (15 of 77) 0.0 % (0 of 2)
Methyl96.5 % (110 of 114)96.5 % (55 of 57)96.5 % (55 of 57)

1. central complement control protein

FRSCEVPTRL NSASLKQPYI TQNYFPVGTV VEYECRPGYR REPSLSPKLT CLQNLKWSTA VEFCKKKSCP NPGEIRNGQI DVPGGILFGA TISFSCNTGY KLFGSTSSFC LISGSSVQWS DPLPECREHH HHHH

Sample

Temperature 310 (±1) K


#NameIsotope labelingTypeConcentration
1central complement control protein[U-98% 15N; U-98% 13C]1 mM

LACS Plot; CA
Referencing offset: -0.4 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.4 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.02 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 42 models in PDB: 1NWV, Strand ID: A Detail


Release date
2002-12-26
Citation 1
Resonance assignments of the central complement control protein module pair of human decay accelerating factor (DAF)
Uhrinova, S.
J. Biomol. NMR (2002), 23, 167-168, PubMed , DOI:
Citation 2
Cooperation between decay-accelerating factor and membrane cofactor protein in protecting cells from autologous complement attack
Brodbeck, W.G., Mold, C., Atkinson, J.P., Medof, M.E.
J. Immunol. (2000), 165, 3999-4006, PubMed 11034410 , DOI 10.1016/s0162-3109(00)80185-x ,
Citation 3
Bulk production and functional analyses of mouse CD55's native and deglycosylated active domains
Lin, F., Immormino, R.M., Shoham, M., Medof, M.E.
Arch. Biochem. Biophys. (2001), 393, 67-72, PubMed 11516162 , DOI 10.1006/abbi.2001.2488 ,
Related entities 1. double module, : 1 : 2 : 249 entities Detail
Interaction partners 1. double module, : 8 interactors Detail
Keywords decay accelerating factor, triple resonance experiments, NMR