BMRB: 5506

Resonance assignments of the central complement control protein module pair of human decay accelerating factor

Authors

Uhrinova, S.

Assembly

double module

Entity

1. double module (polymer, Thiol state: all disulfide bound), 134 monomers, 15021.87 Da Detail

FRSCEVPTRL NSASLKQPYI TQNYFPVGTV VEYECRPGYR REPSLSPKLT CLQNLKWSTA VEFCKKKSCP NPGEIRNGQI DVPGGILFGA TISFSCNTGY KLFGSTSSFC LISGSSVQWS DPLPECREHH HHHH

Formula weight

15021.87 Da

Source organism

Komagataella pastoris

Exptl. method

NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 71.3 %, Completeness (bb): 77.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	71.3 % (1112 of 1560)	78.9 % (643 of 815)	59.4 % (362 of 609)	78.7 % (107 of 136)
Backbone	77.5 % (606 of 782)	94.4 % (252 of 267)	63.0 % (247 of 392)	87.0 % (107 of 123)
Sidechain	68.5 % (618 of 902)	71.4 % (391 of 548)	66.6 % (227 of 341)	0.0 % (0 of 13)
Aromatic	43.0 % (68 of 158)	67.1 % (53 of 79)	19.5 % (15 of 77)	0.0 % (0 of 2)
Methyl	96.5 % (110 of 114)	96.5 % (55 of 57)	96.5 % (55 of 57)

1. central complement control protein

FRSCEVPTRL NSASLKQPYI TQNYFPVGTV VEYECRPGYR REPSLSPKLT CLQNLKWSTA VEFCKKKSCP NPGEIRNGQI DVPGGILFGA TISFSCNTGY KLFGSTSSFC LISGSSVQWS DPLPECREHH HHHH

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Sample

Temperature 310 (±1) K

#	Name	Isotope labeling	Type	Concentration
1	central complement control protein	[U-98% 15N; U-98% 13C]		1 mM

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LACS Plot; CA

Referencing offset: -0.4 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.4 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 42 models in PDB: 1NWV, Strand ID: A Detail

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Release date

2002-12-26

Citation 1

Resonance assignments of the central complement control protein module pair of human decay accelerating factor (DAF)
Uhrinova, S.
J. Biomol. NMR (2002), 23, 167-168, PubMed , DOI:

Show more 2 citaions

Citation 2

Cooperation between decay-accelerating factor and membrane cofactor protein in protecting cells from autologous complement attack
Brodbeck, W.G., Mold, C., Atkinson, J.P., Medof, M.E.
J. Immunol. (2000), 165, 3999-4006, PubMed 11034410 , DOI: , Abstract

Citation 3

Bulk production and functional analyses of mouse CD55's native and deglycosylated active domains
Lin, F., Immormino, R.M., Shoham, M., Medof, M.E.
Arch. Biochem. Biophys. (2001), 393, 67-72, PubMed 11516162 , DOI 10.1006/abbi.2001.2488 , Abstract

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Related entities 1. double module, : 1 : 6 : 382 entities Detail

Interaction partners 1. double module, : 8 interactors Detail

Experiments performed 7 experiments Detail