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Native State Hydrogen Exchange Study of Suppressor and Pathogenic Variants of Transthyretin
Authors
Liu, K., Kelly, J., Wemmer, D.
Assembly
Transthyretin Tetramer
Entity
1. Transthyretin Tetramer (polymer, Thiol state: all free), 128 monomers, 13892.42 × 4 Da Detail

MGPTGTGESK CPLMVKVLDA VRGSPAINVA VHVFRKAADD TWEPFASGKT SESGELHGLT TEEEFVEGIY KVEIDTKSYW KALGISPFHE HAEVVFTAND SGPRRYTIAA LLSPYSYSTT AVVTNPKE


Total weight
55569.68 Da
Max. entity weight
13892.42 Da
Source organism
Homo sapiens
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 28.4 %, Completeness (bb): 50.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All28.4 % (407 of 1433)19.4 % (142 of 731)25.1 % (144 of 573)93.8 % (121 of 129)
Backbone50.0 % (371 of 742)46.4 % (115 of 248)36.6 % (137 of 374)99.2 % (119 of 120)
Sidechain 4.9 % (40 of 809) 5.6 % (27 of 483) 3.5 % (11 of 317)22.2 % (2 of 9)
Aromatic 4.6 % (6 of 130) 4.6 % (3 of 65) 1.6 % (1 of 63)100.0 % (2 of 2)
Methyl 5.6 % (8 of 144) 5.6 % (4 of 72) 5.6 % (4 of 72)

1. Transthyretin

MGPTGTGESK CPLMVKVLDA VRGSPAINVA VHVFRKAADD TWEPFASGKT SESGELHGLT TEEEFVEGIY KVEIDTKSYW KALGISPFHE HAEVVFTAND SGPRRYTIAA LLSPYSYSTT AVVTNPKE

Sample

Temperature 298 (±1) K, pH 5.75 (±0.1)


#NameIsotope labelingTypeConcentration
1Transthyretin0.3 ~ 1.2 mM

Release date
2003-01-26
Citation
Native state hydrogen exchange study of suppressor and pathogenic variants of transthyretin
Liu, K., Kelly, J., Wemmer, D.
J. Mol. Biol. (2002), 320, 821-832, PubMed 12095258 , DOI 10.1016/s0022-2836(02)00471-0 ,
Related entities 1. Transthyretin Tetramer, : 1 : 17 : 1 : 82 : 42 entities Detail
Interaction partners 1. Transthyretin Tetramer, : 51 interactors Detail
Experiments performed 4 experiments Detail
Keywords amyloid, hydrogen exchange, NMR spectroscopy, protection factor, transthyretin