BMRBj - BMREntryMaster

Found 1 Document (1.519 seconds)

BMRB: 5510

3SSG

Native State Hydrogen Exchange Study of Suppressor and Pathogenic Variants of Transthyretin

Authors

Liu, K., Kelly, J., Wemmer, D.

Assembly

Transthyretin

Entity

1. Transthyretin (polymer, Thiol state: all free), 128 monomers, 13876.38 × 4 Da Detail

MGPTGTGESK CPLMVKVLDA VRGSPAINVA VHVFRKAADD TWEPFASGKT SESGEPHGLT TEEEFVEGIY KVEIDTKSYW KALGISPFHE HAEVVFTAND SGPRRYTIAA LLSPYSYSTT AVVTNPKE

Total weight

55505.52 Da

Max. entity weight

13876.38 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 31.6 %, Completeness (bb): 52.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	31.6 % (451 of 1427)	23.5 % (172 of 731)	28.0 % (160 of 572)	96.0 % (119 of 124)
Backbone	52.3 % (392 of 750)	50.2 % (129 of 257)	39.0 % (146 of 374)	98.3 % (117 of 119)
Sidechain	9.3 % (74 of 795)	9.1 % (43 of 474)	9.2 % (29 of 316)	40.0 % (2 of 5)
Aromatic	3.1 % (4 of 130)	3.1 % (2 of 65)	0.0 % (0 of 63)	100.0 % (2 of 2)
Methyl	6.4 % (9 of 140)	5.7 % (4 of 70)	7.1 % (5 of 70)

1. Transthyretin

MGPTGTGESK CPLMVKVLDA VRGSPAINVA VHVFRKAADD TWEPFASGKT SESGEPHGLT TEEEFVEGIY KVEIDTKSYW KALGISPFHE HAEVVFTAND SGPRRYTIAA LLSPYSYSTT AVVTNPKE

Show sample condition

Sample

Temperature 298 (±1) K, pH 7 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Transthyretin	[U-C13; U-N15]		0.3 ~ 1.5 mM
2	D2O			100 %

Release date

2003-01-26

Citation

Native state hydrogen exchange study of suppressor and pathogenic variants of transthyretin
Liu, K., Kelly, J., Wemmer, D.
J. Mol. Biol. (2002), 320, 821-832, PubMed 12095258 , DOI 10.1016/s0022-2836(02)00471-0 , Abstract

Related entities 1. Transthyretin, : 1 : 94 : 71 entities Detail

Interaction partners 1. Transthyretin, : 50 interactors Detail

More details for 50 interactors identified by 11 citations

Experiments performed 4 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5510_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5510_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	TTR_L55P_cs	Warning	357		97.7 (chain: 1, length: 128)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: TTR_L55P_cs
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 128, Sequence coverage: 97.7%
- Total number of assignments
  - ¹³C chemical shifts: 120
  - ¹⁵N chemical shifts: 119
  - ¹H chemical shifts: 118
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	572	120	21.0
¹⁵N chemical shifts	128	119	93.0
¹H chemical shifts	731	118	16.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	256	120	46.9
¹⁵N chemical shifts	119	117	98.3
¹H chemical shifts	257	116	45.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	316	0	0.0
¹⁵N chemical shifts	9	2	22.2
¹H chemical shifts	474	2	0.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	72	0	0.0
¹H chemical shifts	72	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	63	0	0.0
¹⁵N chemical shifts	2	2	100.0
¹H chemical shifts	65	2	3.1

Keywords transthyretin, amyloid, hydrogen exchange, NMR spectroscopy, protection factor

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Found 1 Document (1.519 seconds)

BMRB: 5510

Sample

Related entities 1. Transthyretin, : 1 : 94 : 71 entities Detail

Interaction partners 1. Transthyretin, : 50 interactors Detail

Experiments performed 4 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail