Search

NMR assignment and secondary structure identification of the ribosomal protein L11 from Thermotoga maritima
Authors
Ilin, S., Hoskins, A., Schwalbe, H., Wohnert, J.
Assembly
Thermotoga maritima Ribosomal protein L11
Entity
1. Thermotoga maritima Ribosomal protein L11 (polymer, Thiol state: all free), 141 monomers, 15088.73 Da Detail

MAKKVAAQIK LQLPAGKATP APPVGPALGQ HGVNIMEFCK RFNAETADKA GMILPVVITV YEDKSFTFII KTPPASFLLK KAAGIEKGSS EPKRKIVGKV TRKQIEEIAK TKMPDLNANS LEAAMKIIEG TAKSMGIEVV D


Formula weight
15088.73 Da
Source organism
Thermotoga maritima
Exptl. method
NMR
Data set
assigned_chemical_shifts, coupling_constants
Chem. Shift Complete
Sequence coverage: 97.2 %, Completeness: 94.2 %, Completeness (bb): 94.9 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All94.2 % (1551 of 1647)94.1 % (810 of 861)93.8 % (604 of 644)96.5 % (137 of 142)
Backbone94.9 % (774 of 816)95.6 % (260 of 272)93.5 % (386 of 413)97.7 % (128 of 131)
Sidechain91.3 % (878 of 962)91.9 % (541 of 589)90.9 % (329 of 362)72.7 % (8 of 11)
Aromatic67.7 % (42 of 62)67.7 % (21 of 31)67.7 % (21 of 31)
Methyl94.3 % (166 of 176)94.3 % (83 of 88)94.3 % (83 of 88)

1. Ribosomal protein L11

MAKKVAAQIK LQLPAGKATP APPVGPALGQ HGVNIMEFCK RFNAETADKA GMILPVVITV YEDKSFTFII KTPPASFLLK KAAGIEKGSS EPKRKIVGKV TRKQIEEIAK TKMPDLNANS LEAAMKIIEG TAKSMGIEVV D

Sample #1

Temperature 298 (±0.1) K, pH 6.1 (±0.2)


#NameIsotope labelingTypeConcentration
1Ribosomal protein L11[U-95% 13C; U-95% 15N]1.3 mM
2potassium phosphate25 mM
3potassium chloride50 mM
Sample #2

Temperature 298 (±0.1) K, pH 6.1 (±0.2)


#NameIsotope labelingTypeConcentration
4Ribosomal protein L11[U-15N]1.5 mM
5potassium phosphate25 mM
6potassium chloride50 mM

LACS Plot; CA
Referencing offset: 1.94 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 1.94 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.07 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 1.99 ppm, Outliers: 4 Detail
Coupling constant
111 J values in 1 lists
Temperature 298 (±0.1) K, pH 6.1 (±0.2) Detail
Release date
2003-02-17
Citation 1
NMR assignment of the full-length ribosomal protein L11 from Thermotoga maritima
Ilin, S., Hoskins, A., Schwalbe, H., Wohnert, J.
J. Biomol. NMR (2003), 25, 163-164, PubMed 12652127 , DOI: ,
Citation 2
1H, 15N, and 13C assignments and secondary structure identification for full-length ribosomal protein L11 from Thermus thermophilus
Markus, M.A., Triantafillidou, D., Choli-Papadopoulou, T., Torchia, D.A.
J. Biomol. NMR (2001), 20, 293-294, PubMed 11519754 , DOI: ,
Citation 3
A detailed view of a ribosomal active site: the structure of the L11-RNA complex
Wimberly, B.T., Guymon, R., McCutcheon, J.P., White, S.W., Ramakrishnan, V.
Cell (1999), 97, 491-502, PubMed 10338213 , DOI 10.1016/s0092-8674(00)80759-x ,
Citation 4
The RNA binding domain of ribosomal protein L11: three-dimensional structure of the RNA-bound form of the protein and its interaction with 23 S rRNA
Hinck, A.P., Markus, M.A., Huang, S., Grzesiek, S., Kustonovich, I., Draper, D.E., Torchia, D.A.
J. Mol. Biol. (1997), 274, 101-113, PubMed 9398519 , DOI 10.1006/jmbi.1997.1379 ,
Related entities 1. Thermotoga maritima Ribosomal protein L11, : 1 : 9 : 10 : 209 entities Detail
Interaction partners 1. Thermotoga maritima Ribosomal protein L11, : 1 interactors Detail
Experiments performed 11 experiments Detail