NMR assignment and secondary structure identification of the ribosomal protein L11 from Thermotoga maritima
MAKKVAAQIK LQLPAGKATP APPVGPALGQ HGVNIMEFCK RFNAETADKA GMILPVVITV YEDKSFTFII KTPPASFLLK KAAGIEKGSS EPKRKIVGKV TRKQIEEIAK TKMPDLNANS LEAAMKIIEG TAKSMGIEVV D
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 95.3 % (1567 of 1644) | 95.2 % (820 of 861) | 94.7 % (610 of 644) | 98.6 % (137 of 139) |
Backbone | 96.2 % (795 of 826) | 96.8 % (273 of 282) | 95.2 % (393 of 413) | 98.5 % (129 of 131) |
Sidechain | 94.5 % (897 of 949) | 94.5 % (547 of 579) | 94.5 % (342 of 362) | 100.0 % (8 of 8) |
Aromatic | 83.9 % (52 of 62) | 83.9 % (26 of 31) | 83.9 % (26 of 31) | |
Methyl | 96.6 % (170 of 176) | 96.6 % (85 of 88) | 96.6 % (85 of 88) |
1. Ribosomal protein L11
MAKKVAAQIK LQLPAGKATP APPVGPALGQ HGVNIMEFCK RFNAETADKA GMILPVVITV YEDKSFTFII KTPPASFLLK KAAGIEKGSS EPKRKIVGKV TRKQIEEIAK TKMPDLNANS LEAAMKIIEG TAKSMGIEVV DTemperature 298 (±0.1) K, pH 6.1 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Ribosomal protein L11 | [U-95% 13C; U-95% 15N] | 1.3 mM | |
2 | potassium phosphate | 25 mM | ||
3 | potassium chloride | 50 mM |
Temperature 298 (±0.1) K, pH 6.1 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | Ribosomal protein L11 | [U-15N] | 1.5 mM | |
5 | potassium phosphate | 25 mM | ||
6 | potassium chloride | 50 mM |