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Backbone HN, N, Ca and Cb assignment of the GFPuv mutant, an 54 kDa protein
Authors
Georgescu, J., Rehm, T., Wiehler, J., Steipe, B., Holak, T.A.
Assembly
GFPuv
Entity
1. GFPuv (polymer, Thiol state: all free), 239 monomers, 26822.83 × 2 Da Detail

MGKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKR HDFFKSAMPE GYVQERTISF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YITADKQKNG IKANFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYKG


Total weight
53645.66 Da
Max. entity weight
26822.83 Da
Source organism
Aequorea victoria
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 88.3 %, Completeness: 38.7 %, Completeness (bb): 74.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All38.7 % (1081 of 2795)23.8 % (345 of 1448)50.5 % (550 of 1090)72.4 % (186 of 257)
Backbone74.0 % (1029 of 1390)65.0 % (304 of 468)77.9 % (540 of 693)80.8 % (185 of 229)
Sidechain11.7 % (190 of 1620) 4.1 % (40 of 980)24.5 % (150 of 612) 0.0 % (0 of 28)
Aromatic 0.0 % (0 of 260) 0.0 % (0 of 130) 0.0 % (0 of 129) 0.0 % (0 of 1)
Methyl 5.5 % (13 of 238) 1.7 % (2 of 119) 9.2 % (11 of 119)

1. green fluorescent protein uv

MGKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKR HDFFKSAMPE GYVQERTISF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YITADKQKNG IKANFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYKG

Sample #1

Temperature 310 (±0.5) K, pH 7.0 (±0.1)


#NameIsotope labelingTypeConcentration
1green fluorescent protein uv[U-15N]0.8 ~ 1.5 mM
Sample #2

Temperature 310 (±0.5) K, pH 7.0 (±0.1)


#NameIsotope labelingTypeConcentration
2green fluorescent protein uv[U-15N; U-13C; U-70% 2H]0.8 ~ 1.5 mM

LACS Plot; CA
Referencing offset: 3.71 ppm, Outliers: 2 Detail
LACS Plot; CB
Referencing offset: 3.71 ppm, Outliers: 2 Detail
LACS Plot; HA
Referencing offset: -0.1 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 5.41 ppm, Outliers: 2 Detail
Release date
2003-02-17
Citation
Letter to the editor: Backbone HN, N, Ca and Cb assignment of the GFPuv mutant
Georgescu, J., Rehm, T., Wiehler, J., Steipe, B., Holak, T.A.
J. Biomol. NMR (2003), 25, 161-162
Related entities 1. GFPuv, : 1 : 1 : 107 entities Detail
Interaction partners 1. GFPuv, : 1 interactors Detail
Experiments performed 6 experiments Detail
Keywords green fluorescent protein, deuteration, chromophore