BMRBj - BMREntryMaster

Found 1 Document (2.539 seconds)

BMRB: 5516

1RSF

Assignment of the 1H, 13C and 15N resonances of the Coxsackievirus and Adenovirus receptor domain 1

Authors

Jiang, S., Caffrey, M.

Assembly

Coxsackievirus and Adenovirus receptor

Entity

1. Coxsackievirus and Adenovirus receptor (polymer, Thiol state: all disulfide bound), 126 monomers, 13798.52 Da Detail

GSSITTPEEM IEKAKGETAY LPCKFTLSPE DQGPLDIEWL ISPADNQKVD QVIILYSGDK IYDDYYPDLK GRVHFTSNDL KSGDASINVT NLQLSDIGTY QCKVKKAPGV ANKKIHLVVL VKPSGA

Formula weight

13798.52 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS23:SG	1:CYS102:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.2 %, Completeness (bb): 99.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	99.2 % (1444 of 1455)	99.6 % (753 of 756)	98.9 % (564 of 570)	98.4 % (127 of 129)
Backbone	99.1 % (733 of 740)	99.6 % (252 of 253)	98.6 % (364 of 369)	99.2 % (117 of 118)
Sidechain	99.5 % (828 of 832)	99.6 % (501 of 503)	99.7 % (317 of 318)	90.9 % (10 of 11)
Aromatic	98.9 % (87 of 88)	100.0 % (44 of 44)	100.0 % (43 of 43)	0.0 % (0 of 1)
Methyl	100.0 % (148 of 148)	100.0 % (74 of 74)	100.0 % (74 of 74)

1. Coxsackievirus and Adenovirus

GSSITTPEEM IEKAKGETAY LPCKFTLSPE DQGPLDIEWL ISPADNQKVD QVIILYSGDK IYDDYYPDLK GRVHFTSNDL KSGDASINVT NLQLSDIGTY QCKVKKAPGV ANKKIHLVVL VKPSGA

Show sample condition

Sample #1

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Coxsackievirus and Adenovirus	[U-95% 15N]		0.8 ~ 1.2 mM
2	formate	[U-2H]		50 mM

Sample #2

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
3	Coxsackievirus and Adenovirus	[U-90% 13C; U-90% N15]		0.9 ~ 1.2 mM
4	formate	[U-2H]		50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.802 ppm	internal	direct	1.0
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.74 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 1RSF, Strand ID: A Detail

Release date

2002-09-09

Citation 1

Assignment of the 1H, 13C and 15N resonances of the coxsackievirus and adenovirus receptor domain 1
Jiang, S., Caffrey, M.
J. Biomol. NMR (2002), 24, 365-366, PubMed 12522304 , DOI: , Abstract

Show more 1 citaion

Citation 2

Isolation of a common receptor for Coxsackie B viruses and adenoviruses 2 and 5
Bergelson, J.M., Cunningham, J.A., Droguett, G., Kurt-Jones, E.A., Krithivas, A., Hong, J.S., Horwitz, M.S., Crowell, R.L., Finberg, R.W.
Science (1997), 275, 1320-1323, PubMed 9036860 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. Coxsackievirus and Adenovirus receptor, : 1 : 22 : 243 entities Detail

Interaction partners 1. Coxsackievirus and Adenovirus receptor, : 9 interactors Detail

More details for 9 interactors identified by 6 citations

Experiments performed 13 experiments Detail

1 HNCA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Coxsackievirus and Adenovirus	[U-95% 15N]		0.8 ~ 1.2 mM
2	formate	[U-2H]		50 mM

Sample #2

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
3	Coxsackievirus and Adenovirus	[U-90% 13C; U-90% N15]		0.9 ~ 1.2 mM
4	formate	[U-2H]		50 mM

2 HN(CO)CA

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Coxsackievirus and Adenovirus	[U-95% 15N]		0.8 ~ 1.2 mM
2	formate	[U-2H]		50 mM

Sample #2

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
3	Coxsackievirus and Adenovirus	[U-90% 13C; U-90% N15]		0.9 ~ 1.2 mM
4	formate	[U-2H]		50 mM

3 HNCACB

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Coxsackievirus and Adenovirus	[U-95% 15N]		0.8 ~ 1.2 mM
2	formate	[U-2H]		50 mM

Sample #2

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
3	Coxsackievirus and Adenovirus	[U-90% 13C; U-90% N15]		0.9 ~ 1.2 mM
4	formate	[U-2H]		50 mM

4 CBCA(CO)NH

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Coxsackievirus and Adenovirus	[U-95% 15N]		0.8 ~ 1.2 mM
2	formate	[U-2H]		50 mM

Sample #2

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
3	Coxsackievirus and Adenovirus	[U-90% 13C; U-90% N15]		0.9 ~ 1.2 mM
4	formate	[U-2H]		50 mM

5 HNCO

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Coxsackievirus and Adenovirus	[U-95% 15N]		0.8 ~ 1.2 mM
2	formate	[U-2H]		50 mM

Sample #2

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
3	Coxsackievirus and Adenovirus	[U-90% 13C; U-90% N15]		0.9 ~ 1.2 mM
4	formate	[U-2H]		50 mM

6 H(C)CH-TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Coxsackievirus and Adenovirus	[U-95% 15N]		0.8 ~ 1.2 mM
2	formate	[U-2H]		50 mM

Sample #2

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
3	Coxsackievirus and Adenovirus	[U-90% 13C; U-90% N15]		0.9 ~ 1.2 mM
4	formate	[U-2H]		50 mM

7 (H)CCH-TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Coxsackievirus and Adenovirus	[U-95% 15N]		0.8 ~ 1.2 mM
2	formate	[U-2H]		50 mM

Sample #2

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
3	Coxsackievirus and Adenovirus	[U-90% 13C; U-90% N15]		0.9 ~ 1.2 mM
4	formate	[U-2H]		50 mM

8 CC(CO)NH

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Coxsackievirus and Adenovirus	[U-95% 15N]		0.8 ~ 1.2 mM
2	formate	[U-2H]		50 mM

Sample #2

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
3	Coxsackievirus and Adenovirus	[U-90% 13C; U-90% N15]		0.9 ~ 1.2 mM
4	formate	[U-2H]		50 mM

9 1H-1H TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Coxsackievirus and Adenovirus	[U-95% 15N]		0.8 ~ 1.2 mM
2	formate	[U-2H]		50 mM

Sample #2

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
3	Coxsackievirus and Adenovirus	[U-90% 13C; U-90% N15]		0.9 ~ 1.2 mM
4	formate	[U-2H]		50 mM

10 1H-1H NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Coxsackievirus and Adenovirus	[U-95% 15N]		0.8 ~ 1.2 mM
2	formate	[U-2H]		50 mM

Sample #2

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
3	Coxsackievirus and Adenovirus	[U-90% 13C; U-90% N15]		0.9 ~ 1.2 mM
4	formate	[U-2H]		50 mM

11 1H-15N NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Coxsackievirus and Adenovirus	[U-95% 15N]		0.8 ~ 1.2 mM
2	formate	[U-2H]		50 mM

Sample #2

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
3	Coxsackievirus and Adenovirus	[U-90% 13C; U-90% N15]		0.9 ~ 1.2 mM
4	formate	[U-2H]		50 mM

12 1H-15N TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Coxsackievirus and Adenovirus	[U-95% 15N]		0.8 ~ 1.2 mM
2	formate	[U-2H]		50 mM

Sample #2

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
3	Coxsackievirus and Adenovirus	[U-90% 13C; U-90% N15]		0.9 ~ 1.2 mM
4	formate	[U-2H]		50 mM

13 1H-13C NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
1	Coxsackievirus and Adenovirus	[U-95% 15N]		0.8 ~ 1.2 mM
2	formate	[U-2H]		50 mM

Sample #2

Temperature 295 (±0.1) K, pH 3.0 (±0.02)

#	Name	Isotope labeling	Type	Concentration
3	Coxsackievirus and Adenovirus	[U-90% 13C; U-90% N15]		0.9 ~ 1.2 mM
4	formate	[U-2H]		50 mM

nullKeywords Adenovirus, CAR, Coxsackievirus, NMR assignments

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Found 1 Document (2.539 seconds)

BMRB: 5516

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Chemical shift reference

Related entities 1. Coxsackievirus and Adenovirus receptor, : 1 : 22 : 243 entities Detail

Interaction partners 1. Coxsackievirus and Adenovirus receptor, : 9 interactors Detail

Experiments performed 13 experiments Detail

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