BMRB: 5527

Three-Dimensional Solution Structure of Huwentoxin-Iv by 2D 1H-NMR

Authors

Peng, K., Shu, Q., Liang, S.P.

Assembly

Huwentoxin-IV

Entity

1. Huwentoxin-IV (polymer, Thiol state: all disulfide bound), 35 monomers, 4113.806 Da Detail

ECLEIFKACN PSNDQCCKSS KLVCSRKTRW CKYQI

Formula weight

4113.806 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS17:SG
2	disulfide	sing	1:CYS9:SG	1:CYS24:SG
3	disulfide	sing	1:CYS16:SG	1:CYS31:SG

Source organism

Haplopelma schmidti

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 95.6 %, Completeness (bb): 95.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	95.6 % (216 of 226)	95.6 % (216 of 226)
Backbone	95.7 % (66 of 69)	95.7 % (66 of 69)
Sidechain	95.5 % (150 of 157)	95.5 % (150 of 157)
Aromatic	93.3 % (14 of 15)	93.3 % (14 of 15)
Methyl	100.0 % (12 of 12)	100.0 % (12 of 12)

1. Huwentoxin-IV

ECLEIFKACN PSNDQCCKSS KLVCSRKTRW CKYQI

Show sample condition

Sample

Pressure 1 atm, Temperature 300.00 K, pH 4.50

#	Name	Isotope labeling	Type	Concentration
1	Huwentoxin-IV			5 mM
2	phosphate buffer			0.0 ~ 0.0 mM
3	H2O			90 %
4	D2O			10 %

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Calculated from 20 models in PDB: 1MB6, Strand ID: A Detail

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Release date

2002-09-12

Citation

Function and solution structure of huwentoxin-IV, a potent neuronal tetrodotoxin (TTX)-sensitive sodium channel antagonist from Chinese bird spider Selenocosmia huwena
Peng, K., Shu, Q., Liu, Z., Liang, S.P.
J. Biol. Chem. (2002), 277, 47564-47571, PubMed 12228241 , DOI 10.1074/jbc.M204063200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Huwentoxin-IV, : 1 : 3 : 2 : 66 entities Detail

Experiments performed 4 experiments Detail