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Found 1 Document (1.577 seconds)

BMRB: 5529

1M9G

The structure of G16A MNEI monellin supports a new model of interaction of sweet proteins with the T1R2-T1R3 receptor

Authors

Spadaccini, R., Trabucco, F., Saviano, G., Picone, D., Crescenzi, O., Tancredi, T., Temussi, P.A.

Assembly

Monellin chain B and Monellin chain A

Entity

1. Monellin chain B and Monellin chain A (polymer, Thiol state: all free), 97 monomers, 11416.94 Da Detail

MGEWEIIDIG PFTQNLAKFA VDEENKIGQY GRLTFNKVIR PCMKKTIYEN EGFREIKGYE YQLYVYASDK LFRADISEDY KTRGRKLLRF NGPVPPP

Formula weight

11416.94 Da

Source organism

Dioscoreophyllum cumminsii

Exptl. method

NMR

Refine. method

torsion angle dynamics and restrained energy minimization

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.9 %, Completeness: 81.2 %, Completeness (bb): 96.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	81.2 % (600 of 739)	78.7 % (503 of 639)	97.0 % (97 of 100)
Backbone	96.9 % (278 of 287)	96.9 % (190 of 196)	96.7 % (88 of 91)
Sidechain	71.2 % (322 of 452)	70.7 % (313 of 443)	100.0 % (9 of 9)
Aromatic	43.1 % (28 of 65)	42.2 % (27 of 64)	100.0 % (1 of 1)
Methyl	95.5 % (42 of 44)	95.5 % (42 of 44)

1. monellin

MGEWEIIDIG PFTQNLAKFA VDEENKIGQY GRLTFNKVIR PCMKKTIYEN EGFREIKGYE YQLYVYASDK LFRADISEDY KTRGRKLLRF NGPVPPP

Show sample condition

Sample

Pressure 1 atm, Temperature 308 (±0.1) K, pH 2.9 (±0.1)

#	Name	Concentration
1	monellin	2 mM
2	pottasium phosphate buffer	18.5 mM
3	H2O	90 %
4	D2O	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 1M9G, Strand ID: A Detail

Release date

2003-07-06

Citation

The mechanism of interaction of sweet proteins with the T1R2-T1R3 receptor: evidence from the solution structure of G16A-MNEI
Spadaccini, R., Trabucco, F., Saviano, G., Picone, D., Crescenzi, O., Tancredi, T., Temussi, P.A.
J. Mol. Biol. (2003), 328, 683-692, PubMed 12706725 , DOI: , Abstract

Related entities 1. Monellin chain B and Monellin chain A, : 1 : 1 : 15 : 27 entities Detail

Experiments performed 6 experiments Detail

1 2D NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.1) K, pH 2.9 (±0.1)

#	Name	Concentration
1	monellin	2 mM
2	pottasium phosphate buffer	18.5 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 308 (±0.1) K, pH 2.9 (±0.1)

#	Name	Isotope labeling	Concentration
5	monellin	[U-15N]	2 mM
6	pottasium phosphate buffer		18.5 mM
7	H2O		90 %
8	D2O		10 %

2 3D 15N-separated TOCSY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.1) K, pH 2.9 (±0.1)

#	Name	Concentration
1	monellin	2 mM
2	pottasium phosphate buffer	18.5 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 308 (±0.1) K, pH 2.9 (±0.1)

#	Name	Isotope labeling	Concentration
5	monellin	[U-15N]	2 mM
6	pottasium phosphate buffer		18.5 mM
7	H2O		90 %
8	D2O		10 %

3 3D 15N-separated NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.1) K, pH 2.9 (±0.1)

#	Name	Concentration
1	monellin	2 mM
2	pottasium phosphate buffer	18.5 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 308 (±0.1) K, pH 2.9 (±0.1)

#	Name	Isotope labeling	Concentration
5	monellin	[U-15N]	2 mM
6	pottasium phosphate buffer		18.5 mM
7	H2O		90 %
8	D2O		10 %

4 HNHA

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.1) K, pH 2.9 (±0.1)

#	Name	Concentration
1	monellin	2 mM
2	pottasium phosphate buffer	18.5 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 308 (±0.1) K, pH 2.9 (±0.1)

#	Name	Isotope labeling	Concentration
5	monellin	[U-15N]	2 mM
6	pottasium phosphate buffer		18.5 mM
7	H2O		90 %
8	D2O		10 %

5 HNHB

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.1) K, pH 2.9 (±0.1)

#	Name	Concentration
1	monellin	2 mM
2	pottasium phosphate buffer	18.5 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 308 (±0.1) K, pH 2.9 (±0.1)

#	Name	Isotope labeling	Concentration
5	monellin	[U-15N]	2 mM
6	pottasium phosphate buffer		18.5 mM
7	H2O		90 %
8	D2O		10 %

6 HSQC

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 atm, Temperature 308 (±0.1) K, pH 2.9 (±0.1)

#	Name	Concentration
1	monellin	2 mM
2	pottasium phosphate buffer	18.5 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 308 (±0.1) K, pH 2.9 (±0.1)

#	Name	Isotope labeling	Concentration
5	monellin	[U-15N]	2 mM
6	pottasium phosphate buffer		18.5 mM
7	H2O		90 %
8	D2O		10 %

nullKeywords 5 STRANDED BETA SHEET 1 HELIX

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Found 1 Document (1.577 seconds)

BMRB: 5529

Sample

Related entities 1. Monellin chain B and Monellin chain A, : 1 : 1 : 15 : 27 entities Detail

Experiments performed 6 experiments Detail

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