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The structure of G16A MNEI monellin supports a new model of interaction of sweet proteins with the T1R2-T1R3 receptor
Authors
Spadaccini, R., Trabucco, F., Saviano, G., Picone, D., Crescenzi, O., Tancredi, T., Temussi, P.A.
Assembly
Monellin chain B and Monellin chain A
Entity
1. Monellin chain B and Monellin chain A (polymer, Thiol state: all free), 97 monomers, 11416.94 Da Detail

MGEWEIIDIG PFTQNLAKFA VDEENKIGQY GRLTFNKVIR PCMKKTIYEN EGFREIKGYE YQLYVYASDK LFRADISEDY KTRGRKLLRF NGPVPPP


Formula weight
11416.94 Da
Source organism
Dioscoreophyllum cumminsii
Exptl. method
NMR
Refine. method
torsion angle dynamics and restrained energy minimization
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 96.9 %, Completeness: 81.8 %, Completeness (bb): 96.8 % Detail

Polymer type: polypeptide(L)

Total1H15N
All81.8 % (610 of 746)79.2 % (506 of 639)97.2 % (104 of 107)
Backbone96.8 % (270 of 279)96.8 % (182 of 188)96.7 % (88 of 91)
Sidechain72.8 % (340 of 467)71.8 % (324 of 451)100.0 % (16 of 16)
Aromatic46.2 % (30 of 65)45.3 % (29 of 64)100.0 % (1 of 1)
Methyl95.5 % (42 of 44)95.5 % (42 of 44)

1. monellin

MGEWEIIDIG PFTQNLAKFA VDEENKIGQY GRLTFNKVIR PCMKKTIYEN EGFREIKGYE YQLYVYASDK LFRADISEDY KTRGRKLLRF NGPVPPP

Sample

Pressure 1 atm, Temperature 308 (±0.1) K, pH 2.9 (±0.1)


#NameIsotope labelingTypeConcentration
1monellin2 mM
2pottasium phosphate buffer18.5 mM
3H2O90 %
4D2O10 %

Protein Blocks Logo
Calculated from 20 models in PDB: 1M9G, Strand ID: A Detail


Release date
2003-07-06
Citation
The mechanism of interaction of sweet proteins with the T1R2-T1R3 receptor: evidence from the solution structure of G16A-MNEI
Spadaccini, R., Trabucco, F., Saviano, G., Picone, D., Crescenzi, O., Tancredi, T., Temussi, P.A.
J. Mol. Biol. (2003), 328, 683-692, PubMed 12706725 , DOI 10.1016/s0022-2836(03)00346-2 ,
Related entities 1. Monellin chain B and Monellin chain A, : 1 : 1 : 13 : 13 entities Detail
Keywords 5 STRANDED BETA SHEET 1 HELIX