BMRB: 5566

1H, 15N and 13C Resonance Assignments for the PTB Domain of the Signaling Protein Shc

Authors

Farooq, A., Zeng, L., Zhou, M.

Assembly

Phosphotyrosine binding domain of Shc

Entity

1. Phosphotyrosine binding domain of Shc (polymer, Thiol state: not reported), 207 monomers, 22625.73 Da Detail

MNKLSGGGGR RTRVEGGQLG GEEWTRHGSF VNKPTRGWLH PNDKVMGPGV SYLVRYMGCV EVLQSMRALD FNTRTQVTRE AISLVCEAVP GAKGATRRRK PCSRPLSSIL GRSNLKFAGM PITLTVSTSS LNLMAADCKQ IIANHHMQSI SFASGGDPDT AEYVAYVAKD PVNQRACHIL ECPEGLAQDV ISTIGQAFEL RFKQYLR

Formula weight

22625.73 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Distance Geometry, Simulated Annealing, Molecular Dynamics, ARIA

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 89.9 %, Completeness: 60.5 %, Completeness (bb): 71.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	60.5 % (1414 of 2338)	60.7 % (739 of 1218)	55.5 % (502 of 904)	80.1 % (173 of 216)
Backbone	71.2 % (870 of 1222)	80.0 % (340 of 425)	60.0 % (360 of 600)	86.3 % (170 of 197)
Sidechain	53.5 % (697 of 1302)	50.2 % (398 of 793)	60.4 % (296 of 490)	15.8 % (3 of 19)
Aromatic	29.9 % (43 of 144)	31.9 % (23 of 72)	25.7 % (18 of 70)	100.0 % (2 of 2)
Methyl	78.2 % (172 of 220)	84.5 % (93 of 110)	71.8 % (79 of 110)

1. Phosphotyrosine Binding Domain

MNKLSGGGGR RTRVEGGQLG GEEWTRHGSF VNKPTRGWLH PNDKVMGPGV SYLVRYMGCV EVLQSMRALD FNTRTQVTRE AISLVCEAVP GAKGATRRRK PCSRPLSSIL GRSNLKFAGM PITLTVSTSS LNLMAADCKQ IIANHHMQSI SFASGGDPDT AEYVAYVAKD PVNQRACHIL ECPEGLAQDV ISTIGQAFEL RFKQYLR

Show sample condition

Sample

Temperature 308 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Phosphotyrosine Binding Domain			0.5 mM

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LACS Plot; CA

Referencing offset: 0.11 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	external	indirect	1.0

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LACS Plot; CB

Referencing offset: 0.11 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	external	indirect	1.0

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LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	external	indirect	1.0

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Protein Blocks Logo

Calculated from 1 models in PDB: 1N3H, Strand ID: A Detail

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Release date

2003-03-17

Citation

1H, 15N and 13C resonance assignments for the PTB domain of the signaling protein Shc
Farooq, A., Zeng, L., Zhou, M.
J. Biomol. NMR (2003), 25, 255-256, PubMed 12652140 , Abstract

Related entities 1. Phosphotyrosine binding domain of Shc, : 1 : 7 : 4 : 67 entities Detail

Interaction partners 1. Phosphotyrosine binding domain of Shc, : 94 interactors Detail

Experiments performed 1 experiments Detail