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Found 1 Document (6.382 seconds)

BMRB: 5610

1MV4

The Structure of the Carboxyl Terminus of Striated alpha-Tropomyosin in Solution Reveals an Unusual Parallel Arrangement of Interacting alpha-Helices

Authors

Greenfield, N.J., Swapna, G., Huang, Y., Palm, T., Graboski, J., Montelione, G.T., Hitchcock-DeGregori, S.E.

Assembly

C terminus of rat striated muscle alpha tropomyosin encoded by exon 9a

Entity

1. C terminus of rat striated muscle alpha tropomyosin encoded by exon 9a (polymer, Thiol state: all disulfide bound), 37 monomers, 4171.656 × 2 Da Detail

GCGKSIDDLE DELYAQKLKY KAISEELDHA LKDMTSI

Total weight

8343.312 Da

Max. entity weight

4171.656 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS2:SG	1:CYS2:SG

Source organism

Rattus norvegicus

Exptl. method

NMR

Refine. method

1648 CONFORMATIONALLY- RESTRICTING NOE CONSTRAINTS (OUT OF A TOTAL OF 2036 NOE DERIVED DISTANCES) 52 LOOSE DIHEDRAL ANGLE CONSTRAINTS AND 48 BACKBONE INTRA- HELICAL HYDROGEN BOND CONSTRAINTS WERE UTILILIZED.

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 98.6 %, Completeness (bb): 97.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	98.6 % (420 of 426)	99.5 % (221 of 222)	97.6 % (162 of 166)	97.4 % (37 of 38)
Backbone	97.3 % (216 of 222)	98.7 % (75 of 76)	96.3 % (105 of 109)	97.3 % (36 of 37)
Sidechain	100.0 % (239 of 239)	100.0 % (146 of 146)	100.0 % (92 of 92)	100.0 % (1 of 1)
Aromatic	100.0 % (20 of 20)	100.0 % (10 of 10)	100.0 % (10 of 10)
Methyl	100.0 % (40 of 40)	100.0 % (20 of 20)	100.0 % (20 of 20)

1. residues 251-284 of rat striated muscle tropomyosin

GCGKSIDDLE DELYAQKLKY KAISEELDHA LKDMTSI

Show sample condition

Sample #1

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N]	0.4 ~ 1.2 mM
2	NaCl		100 mM
3	Na phosphate		10 mM

Sample #2

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	residues 251-284 of rat striated muscle tropomyosin	[U-99% 13C; U-98% 15N]		0.4 ~ 1.2 mM

Sample #3

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N; U-99% 13C]		0.4 mM
6	residues 251-284 of rat striated muscle tropomyosin			0.4 mM

Sample #4

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	residues 251-284 of rat striated muscle tropomyosin			0.8 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 1MV4, Strand ID: A, B Detail

Coupling constant

26 J values in 1 lists
Temperature 183 (±0.2) K, pH 6.5 (±0.1) Detail

Release date

2003-02-20

Citation

The structure of the carboxyl terminus of striated alpha-tropomyosin in solution reveals an unusual parallel arrangement of interacting alpha-helices
Greenfield, N.J., Swapna, G., Huang, Y., Palm, T., Graboski, S., Montelione, G.T., Hitchcock-DeGregori, S.E.
Biochemistry (2003), 42, 614-619, PubMed 12534273 , DOI 10.1021/bi026989e , Abstract

Related entities 1. C terminus of rat striated muscle alpha tropomyosin encoded by exon 9a, : 1 : 1 : 15 : 68 entities Detail

Interaction partners 1. C terminus of rat striated muscle alpha tropomyosin encoded by exon 9a, : 2 interactors Detail

More details for 2 interactors identified by 2 citations

Experiments performed 15 experiments Detail

1 15N-1H HSQC

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N]	0.4 ~ 1.2 mM
2	NaCl		100 mM
3	Na phosphate		10 mM

Sample #2

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	residues 251-284 of rat striated muscle tropomyosin	[U-99% 13C; U-98% 15N]		0.4 ~ 1.2 mM

Sample #3

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N; U-99% 13C]		0.4 mM
6	residues 251-284 of rat striated muscle tropomyosin			0.4 mM

Sample #4

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	residues 251-284 of rat striated muscle tropomyosin			0.8 mM

2 (HA)(CA)CO(CA)NH

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N]	0.4 ~ 1.2 mM
2	NaCl		100 mM
3	Na phosphate		10 mM

Sample #2

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	residues 251-284 of rat striated muscle tropomyosin	[U-99% 13C; U-98% 15N]		0.4 ~ 1.2 mM

Sample #3

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N; U-99% 13C]		0.4 mM
6	residues 251-284 of rat striated muscle tropomyosin			0.4 mM

Sample #4

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	residues 251-284 of rat striated muscle tropomyosin			0.8 mM

3 HNC0

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N]	0.4 ~ 1.2 mM
2	NaCl		100 mM
3	Na phosphate		10 mM

Sample #2

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	residues 251-284 of rat striated muscle tropomyosin	[U-99% 13C; U-98% 15N]		0.4 ~ 1.2 mM

Sample #3

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N; U-99% 13C]		0.4 mM
6	residues 251-284 of rat striated muscle tropomyosin			0.4 mM

Sample #4

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	residues 251-284 of rat striated muscle tropomyosin			0.8 mM

4 HN(CO)CA

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N]	0.4 ~ 1.2 mM
2	NaCl		100 mM
3	Na phosphate		10 mM

Sample #2

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	residues 251-284 of rat striated muscle tropomyosin	[U-99% 13C; U-98% 15N]		0.4 ~ 1.2 mM

Sample #3

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N; U-99% 13C]		0.4 mM
6	residues 251-284 of rat striated muscle tropomyosin			0.4 mM

Sample #4

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	residues 251-284 of rat striated muscle tropomyosin			0.8 mM

5 HNCA

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N]	0.4 ~ 1.2 mM
2	NaCl		100 mM
3	Na phosphate		10 mM

Sample #2

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	residues 251-284 of rat striated muscle tropomyosin	[U-99% 13C; U-98% 15N]		0.4 ~ 1.2 mM

Sample #3

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N; U-99% 13C]		0.4 mM
6	residues 251-284 of rat striated muscle tropomyosin			0.4 mM

Sample #4

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	residues 251-284 of rat striated muscle tropomyosin			0.8 mM

6 CBCANH

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N]	0.4 ~ 1.2 mM
2	NaCl		100 mM
3	Na phosphate		10 mM

Sample #2

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	residues 251-284 of rat striated muscle tropomyosin	[U-99% 13C; U-98% 15N]		0.4 ~ 1.2 mM

Sample #3

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N; U-99% 13C]		0.4 mM
6	residues 251-284 of rat striated muscle tropomyosin			0.4 mM

Sample #4

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	residues 251-284 of rat striated muscle tropomyosin			0.8 mM

7 CBCA(CO)NH

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N]	0.4 ~ 1.2 mM
2	NaCl		100 mM
3	Na phosphate		10 mM

Sample #2

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	residues 251-284 of rat striated muscle tropomyosin	[U-99% 13C; U-98% 15N]		0.4 ~ 1.2 mM

Sample #3

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N; U-99% 13C]		0.4 mM
6	residues 251-284 of rat striated muscle tropomyosin			0.4 mM

Sample #4

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	residues 251-284 of rat striated muscle tropomyosin			0.8 mM

8 HA(CA)NH

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N]	0.4 ~ 1.2 mM
2	NaCl		100 mM
3	Na phosphate		10 mM

Sample #2

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	residues 251-284 of rat striated muscle tropomyosin	[U-99% 13C; U-98% 15N]		0.4 ~ 1.2 mM

Sample #3

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N; U-99% 13C]		0.4 mM
6	residues 251-284 of rat striated muscle tropomyosin			0.4 mM

Sample #4

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	residues 251-284 of rat striated muscle tropomyosin			0.8 mM

9 HA(CA)(CO)CANH

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N]	0.4 ~ 1.2 mM
2	NaCl		100 mM
3	Na phosphate		10 mM

Sample #2

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	residues 251-284 of rat striated muscle tropomyosin	[U-99% 13C; U-98% 15N]		0.4 ~ 1.2 mM

Sample #3

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N; U-99% 13C]		0.4 mM
6	residues 251-284 of rat striated muscle tropomyosin			0.4 mM

Sample #4

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	residues 251-284 of rat striated muscle tropomyosin			0.8 mM

10 CCH-COSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N]	0.4 ~ 1.2 mM
2	NaCl		100 mM
3	Na phosphate		10 mM

Sample #2

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	residues 251-284 of rat striated muscle tropomyosin	[U-99% 13C; U-98% 15N]		0.4 ~ 1.2 mM

Sample #3

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N; U-99% 13C]		0.4 mM
6	residues 251-284 of rat striated muscle tropomyosin			0.4 mM

Sample #4

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	residues 251-284 of rat striated muscle tropomyosin			0.8 mM

11 HCCH-COSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N]	0.4 ~ 1.2 mM
2	NaCl		100 mM
3	Na phosphate		10 mM

Sample #2

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	residues 251-284 of rat striated muscle tropomyosin	[U-99% 13C; U-98% 15N]		0.4 ~ 1.2 mM

Sample #3

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N; U-99% 13C]		0.4 mM
6	residues 251-284 of rat striated muscle tropomyosin			0.4 mM

Sample #4

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	residues 251-284 of rat striated muscle tropomyosin			0.8 mM

12 2QF-COSY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N]	0.4 ~ 1.2 mM
2	NaCl		100 mM
3	Na phosphate		10 mM

Sample #2

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	residues 251-284 of rat striated muscle tropomyosin	[U-99% 13C; U-98% 15N]		0.4 ~ 1.2 mM

Sample #3

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N; U-99% 13C]		0.4 mM
6	residues 251-284 of rat striated muscle tropomyosin			0.4 mM

Sample #4

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	residues 251-284 of rat striated muscle tropomyosin			0.8 mM

13 15N Edited NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N]	0.4 ~ 1.2 mM
2	NaCl		100 mM
3	Na phosphate		10 mM

Sample #2

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	residues 251-284 of rat striated muscle tropomyosin	[U-99% 13C; U-98% 15N]		0.4 ~ 1.2 mM

Sample #3

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N; U-99% 13C]		0.4 mM
6	residues 251-284 of rat striated muscle tropomyosin			0.4 mM

Sample #4

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	residues 251-284 of rat striated muscle tropomyosin			0.8 mM

14 13C Edited NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N]	0.4 ~ 1.2 mM
2	NaCl		100 mM
3	Na phosphate		10 mM

Sample #2

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	residues 251-284 of rat striated muscle tropomyosin	[U-99% 13C; U-98% 15N]		0.4 ~ 1.2 mM

Sample #3

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N; U-99% 13C]		0.4 mM
6	residues 251-284 of rat striated muscle tropomyosin			0.4 mM

Sample #4

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	residues 251-284 of rat striated muscle tropomyosin			0.8 mM

15 13C X-Filtered NOESY

Instrument

#1 Model Varian INOVA (500 MHz)

#2 Model Varian INOVA (600 MHz)

Sample #1

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Concentration
1	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N]	0.4 ~ 1.2 mM
2	NaCl		100 mM
3	Na phosphate		10 mM

Sample #2

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	residues 251-284 of rat striated muscle tropomyosin	[U-99% 13C; U-98% 15N]		0.4 ~ 1.2 mM

Sample #3

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
5	residues 251-284 of rat striated muscle tropomyosin	[U-98% 15N; U-99% 13C]		0.4 mM
6	residues 251-284 of rat striated muscle tropomyosin			0.4 mM

Sample #4

Temperature 183 (±0.2) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
7	residues 251-284 of rat striated muscle tropomyosin			0.8 mM

nullKeywords coiled coil, tropomyosin, troponin interaction site

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Found 1 Document (6.382 seconds)

BMRB: 5610

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Related entities 1. C terminus of rat striated muscle alpha tropomyosin encoded by exon 9a, : 1 : 1 : 15 : 68 entities Detail

Interaction partners 1. C terminus of rat striated muscle alpha tropomyosin encoded by exon 9a, : 2 interactors Detail

Experiments performed 15 experiments Detail

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