BMRB: 5620

Assignment for ribosomal protein S28E

Authors

Wu, B., Yee, A., Arrowsmith, C.

Assembly

ribosomal protein S28E

Entity

1. ribosomal protein S28E (polymer, Thiol state: all other bound), 68 monomers, 7666.964 Da Detail

MDDATPAEVI EVLKRTGMTG EVMQVKCRIL DGRDKGRILT RNVMGPIREG DILMLLDTIR EAKEIRTP

Formula weight

7666.964 Da

Source organism

Methanothermobacter thermautotrophicus

Exptl. method

NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.6 %, Completeness: 92.0 %, Completeness (bb): 88.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	92.0 % (721 of 784)	93.0 % (384 of 413)	91.8 % (279 of 304)	86.6 % (58 of 67)
Backbone	88.8 % (357 of 402)	89.9 % (125 of 139)	88.9 % (176 of 198)	86.2 % (56 of 65)
Sidechain	95.0 % (422 of 444)	94.5 % (259 of 274)	95.8 % (161 of 168)	100.0 % (2 of 2)
Methyl	96.7 % (87 of 90)	95.6 % (43 of 45)	97.8 % (44 of 45)

1. ribosomal protein S28E

MDDATPAEVI EVLKRTGMTG EVMQVKCRIL DGRDKGRILT RNVMGPIREG DILMLLDTIR EAKEIRTP

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	ribosomal protein S28E	[U-13C; U-15N]		1 mM
2	NaCl			450 mM
3	Na2PO4			25 mM

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LACS Plot; CA

Referencing offset: -0.36 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: -0.36 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.07 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CO

Referencing offset: 0.29 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 20 models in PDB: 1NE3, Strand ID: A Detail

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Release date

2002-12-26

Citation

Solution structure of ribosomal protein S28E from Methanobacterium thermoautotrophicum
Wu, B., Yee, A., Arrowsmith, C.
Protein Sci. (2003), 12, 2831-2837, PubMed 14627743 , DOI 10.1110/ps.03358203 , Abstract

Related entities 1. ribosomal protein S28E, : 1 : 1 : 237 entities Detail

Experiments performed 2 experiments Detail