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BMRB: 5623

Assignments of 1H, 15N and 13C resonances of the proline-rich matrix protein of Moloney Murine Leukemia Virus (MA Mo-MuLV)

Authors

Noskova, V.N., Rogov, V.V.

Assembly

matrix protein from Moloney Murine Leukemia Virus matrix protein

Entity

1. matrix protein from Moloney Murine Leukemia Virus matrix protein (polymer, Thiol state: all free), 131 monomers, 14800.81 Da Detail

MGQTVTTPLS LTLGHWKDVE RIAHNQSVDV KKRRWVTFCS AEWPTFNVGW PRDGTFNRDL ITQVKIKVFS PGPHGHPDQV PYIVTWEALA FDPPPWVKPF VHPKPPPPLP PSAPSLPLEP PRSTPPRSSL Y

Formula weight

14800.81 Da

Source organism

Murine leukemia virus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 90.1 %, Completeness (bb): 97.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.1 % (1425 of 1581)	85.4 % (709 of 830)	94.8 % (598 of 631)	98.3 % (118 of 120)
Backbone	97.0 % (716 of 738)	97.5 % (238 of 244)	96.4 % (373 of 387)	98.1 % (105 of 107)
Sidechain	85.7 % (830 of 968)	80.4 % (471 of 586)	93.8 % (346 of 369)	100.0 % (13 of 13)
Aromatic	89.3 % (150 of 168)	79.8 % (67 of 84)	98.7 % (77 of 78)	100.0 % (6 of 6)
Methyl	96.2 % (125 of 130)	95.4 % (62 of 65)	96.9 % (63 of 65)

1. matrix protein

MGQTVTTPLS LTLGHWKDVE RIAHNQSVDV KKRRWVTFCS AEWPTFNVGW PRDGTFNRDL ITQVKIKVFS PGPHGHPDQV PYIVTWEALA FDPPPWVKPF VHPKPPPPLP PSAPSLPLEP PRSTPPRSSL Y

Show sample condition

Sample

Temperature 303 (±1) K, pH 5.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	matrix protein	[U-95% 13C; U-90% 15N]		1.0 ~ 1.2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.01 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.0 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.02 ppm, Outliers: 1 Detail

Release date

2003-03-17

Citation

Assignments of 1H, 15N and 13C resonances of the proline-rich matrix protein of Moloney murine leukemia virus (MA MoMuLV)
Noskova, V.N., Rogov, V.V., Lohr, F., Rozenberg, Y., Anderson, W.FRENCH., Potekhin, S.A., Ruterjans, H.
J. Biomol. NMR (2003), 25, 261-262, PubMed 12652143 , DOI: , Abstract

Related entities 1. matrix protein from Moloney Murine Leukemia Virus matrix protein, : 2 : 2 : 20 entities Detail

Interaction partners 1. matrix protein from Moloney Murine Leukemia Virus matrix protein, : 6 interactors Detail

More details for 6 interactors identified by 2 citations

Experiments performed 15 experiments Detail

1 [15N,1H]-TROSY-HNCO