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1H, 15N and 13C backbone assignment of the Green Fluorescent Protein (GFP)
Authors
Khan, F., Stott, K., Jackson, S.
Assembly
truncated GFPuv
Entity
1. truncated GFPuv (polymer, Thiol state: all free), 229 monomers, 25646.46 Da Detail

GSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKR HDFFKSAMPE GYVQERTISF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YITADKQKNG IKANFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGI


Formula weight
25646.46 Da
Entity Connection
covalent 3 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1covalentdoub0:SER65:C1:TYR66:N
2covalentsing0:SER65:C1:GLY67:N
3covalentdoub0:TYR66:CA1:TYR66:CB

Source organism
Aequorea victoria
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 93.4 %, Completeness: 38.4 %, Completeness (bb): 73.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All38.4 % (1029 of 2678)16.7 % (232 of 1387)57.1 % (596 of 1044)81.4 % (201 of 247)
Backbone73.3 % (977 of 1332)42.0 % (188 of 448)88.6 % (589 of 665)91.3 % (200 of 219)
Sidechain15.3 % (238 of 1553) 4.8 % (45 of 939)32.8 % (192 of 586) 3.6 % (1 of 28)
Aromatic 0.8 % (2 of 248) 0.8 % (1 of 124) 0.0 % (0 of 123)100.0 % (1 of 1)
Methyl 8.2 % (19 of 232) 4.3 % (5 of 116)12.1 % (14 of 116)

1. Green Fluorescent Protein

GSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKR HDFFKSAMPE GYVQERTISF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YITADKQKNG IKANFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGI

Sample #1

Temperature 310 (±0.5) K, pH 7.2 (±0.1)


#NameIsotope labelingTypeConcentration
1Green Fluorescent Protein[U-95% 13C; U-95% 15N]1.0 mM
2sodium chloride137 mM
3potassium chloride2.7 mM
4sodium phosphate6.0 mM
Sample #2

Temperature 310 (±0.5) K, pH 7.2 (±0.1)


#NameIsotope labelingTypeConcentration
5Green Fluorescent Protein[U-95% 13C; U-95% 15N; U-75% 2H]1.0 mM
6sodium chloride137 mM
7potassium chloride2.7 mM
8sodium phosphate6.0 mM

LACS Plot; CA
Referencing offset: -0.01 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: -0.01 ppm, Outliers: 3 Detail
LACS Plot; CO
Referencing offset: 0.44 ppm, Outliers: 1 Detail
Release date
2003-06-09
Citation
Letter to the Editor: 1H, 15N and 13C backbone assignment of the Green Fluorescent Protein (GFP)
Khan, F., Stott, K., Jackson, S.
J. Biomol. NMR (2003), 26, 281-282
Related entities 1. truncated GFPuv, : 1 : 1 : 109 entities Detail
Interaction partners 1. truncated GFPuv, : 1 interactors Detail
Experiments performed 5 experiments Detail
Keywords GFP, assignment, NMR, chromophore