Structure and Ubiquitin Interactions of the Conserved NZF Domain of Npl4
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | metal coordination | sing | 1:CYS9:SG | 2:ZN1:ZN |
2 | metal coordination | sing | 1:CYS12:SG | 2:ZN1:ZN |
3 | metal coordination | sing | 1:CYS23:SG | 2:ZN1:ZN |
4 | metal coordination | sing | 1:CYS26:SG | 2:ZN1:ZN |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 89.0 % (292 of 328) | 88.8 % (150 of 169) | 88.1 % (111 of 126) | 93.9 % (31 of 33) |
Backbone | 91.8 % (167 of 182) | 92.1 % (58 of 63) | 91.1 % (82 of 90) | 93.1 % (27 of 29) |
Sidechain | 87.4 % (152 of 174) | 86.8 % (92 of 106) | 87.5 % (56 of 64) | 100.0 % (4 of 4) |
Aromatic | 73.3 % (22 of 30) | 73.3 % (11 of 15) | 71.4 % (10 of 14) | 100.0 % (1 of 1) |
Methyl | 100.0 % (16 of 16) | 100.0 % (8 of 8) | 100.0 % (8 of 8) |
1. Npl4 Zinc Finger
GSTSAMWACQ HCTFMNQPGT GHCEMCSLPR TPressure 1 atm, Temperature 291 (±0.1) K, pH 5.5 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Npl4 Zinc Finger | [U-15N; U-13C] | 1 mM | |
2 | sodium phosphate | 20 mM | ||
3 | NaCl | 50 mM | ||
4 | BME | 5 mM | ||
5 | H2O | 90 % | ||
6 | D2O | 10 % |