BMRB: 5677

1H, 13C, and 15N assignment of the Focal Adhesion Targeting Domain of Focal Adhesion Kinase

Authors

Prutzman, K.C., Gao, G., Schaller, M.D., Campbell, S.L.

Assembly

focal adhesion targeting domain of focal adhesion kinase

Entity

1. focal adhesion targeting domain of focal adhesion kinase (polymer, Thiol state: not present), 146 monomers, 15878.27 Da Detail

GSPGISGGGG GIRSNDKVYE NVTGLVKAVI EMSSKIQPAP PEEYVPMVKE VGLALRTLLA TVDESLPVLP ASTHREIEMA QKLLNSDLAE LINKMKLAQQ YVMTSLQQEY KKQMLTAAHA LAVDAKNLLD VIDQARLKMI SQSRPH

Formula weight

15878.27 Da

Source organism

Gallus gallus

Exptl. method

NMR

Refine. method

distance geometry and torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.6 %, Completeness: 86.8 %, Completeness (bb): 91.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	86.8 % (1457 of 1678)	83.8 % (733 of 875)	88.9 % (579 of 651)	95.4 % (145 of 152)
Backbone	91.0 % (783 of 860)	90.1 % (264 of 293)	90.4 % (388 of 429)	94.9 % (131 of 138)
Sidechain	83.1 % (794 of 955)	79.7 % (464 of 582)	88.3 % (317 of 359)	92.9 % (13 of 14)
Aromatic	79.5 % (35 of 44)	86.4 % (19 of 22)	72.7 % (16 of 22)
Methyl	84.9 % (163 of 192)	79.2 % (76 of 96)	90.6 % (87 of 96)

1. focal adhesion targeting domain

GSPGISGGGG GIRSNDKVYE NVTGLVKAVI EMSSKIQPAP PEEYVPMVKE VGLALRTLLA TVDESLPVLP ASTHREIEMA QKLLNSDLAE LINKMKLAQQ YVMTSLQQEY KKQMLTAAHA LAVDAKNLLD VIDQARLKMI SQSRPH

Show sample condition

Sample

Temperature 310 (±0.2) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	focal adhesion targeting domain	[U-13C; U-15N]		0.85 mM

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LACS Plot; CA

Referencing offset: -0.33 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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LACS Plot; CB

Referencing offset: -0.33 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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LACS Plot; HA

Referencing offset: -0.2 ppm, Outliers: 4 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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LACS Plot; CO

Referencing offset: -0.29 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

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Protein Blocks Logo

Calculated from 25 models in PDB: 1KTM, Strand ID: A Detail

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Release date

2004-03-14

Citation

NMR solution structure of the focal adhesion targeting domain of focal adhesion kinase in complex with a paxillin LD peptide: evidence for a two-site binding model
Gao, G., Prutzman, K.C., King, M.L., Scheswohl, D.M., DeRose, E.F., London, R.E., Schaller, M.D., Campbell, S.L.
J. Biol. Chem. (2004), 279, 8441-8451, PubMed 14662767 , DOI 10.1074/jbc.M309808200 , Abstract

Related entities 1. focal adhesion targeting domain of focal adhesion kinase, : 1 : 1 : 5 : 41 entities Detail

Interaction partners 1. focal adhesion targeting domain of focal adhesion kinase, : 6 interactors Detail