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BMRB: 5693

Backbone chemical shift assignments of Grb2 complexed with ligand peptides for SH3 and SH2 domains

Authors

Yuzawa, S., Ogura, K., Hatanaka, H., Miura, K., Inagaki, F.

Assembly

Ligand loaded growth factor receptor-bound protein 2

Entity

1. growth factor receptor-bound protein 2 (polymer, Thiol state: all free), 217 monomers, 25157.90 Da Detail

MEAIAKYDFK ATADDELSFK RGDILKVLNE ESDQNWYKAE LNGKDGFIPK NYIEMKPHPW FFGKIPRAKA EEMLSKQRHD GAFLIRESES APGDFSLSVK FGNDVQHFKV LRDGAGKYFL WVVKFNSLNE LVDYHRSTSV SRNQQIFLRD IEQVPQQPTY VQALFDFDPQ EDGELGFRRG DFIHVMDNSD PNWWKGAAHG QTGMFPRNYV TPVNRNV

2. EGF Receptor phosphorylated peptide (polymer, Thiol state: not present), 7 monomers, 931.8793 Da Detail

EXINSQV

3. Sos peptide (polymer, Thiol state: not present), 10 monomers, 1170.408 Da Detail

VPPPVPPRRR

Total weight

27260.188 Da

Max. entity weight

25157.9 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 90.6 %, Completeness: 41.9 %, Completeness (bb): 75.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	41.9 % (1185 of 2828)	23.5 % (349 of 1488)	58.8 % (642 of 1092)	78.2 % (194 of 248)
Backbone	75.9 % (1037 of 1366)	50.5 % (235 of 465)	89.0 % (609 of 684)	88.9 % (193 of 217)
Sidechain	20.2 % (339 of 1680)	11.0 % (113 of 1023)	35.9 % (225 of 626)	3.2 % (1 of 31)
Aromatic	4.2 % (13 of 310)	7.1 % (11 of 155)	1.3 % (2 of 150)	0.0 % (0 of 5)
Methyl	23.0 % (46 of 200)	18.0 % (18 of 100)	28.0 % (28 of 100)

1. growth factor receptor-bound protein 2

2. EGF Receptor phosphorylated peptide

EXINSQV

3. Sos peptide

VPPPVPPRRR

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 7.2 (±0.05)

#	Name	Isotope labeling	Concentration
1	growth factor receptor-bound protein 2	[U-80% 2H; U-98% 13C; U-98% 15N]	1.2 mM
2	EGF Receptor phosphorylated peptide		1.44 mM
3	Sos peptide		6.0 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: 0.23 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: 0.23 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	0.0 ppm	internal	direct	1.0

Referencing offset: 0.47 ppm, Outliers: 1 Detail

Release date

2003-08-06

Citation 1

Backbone assignments of Grb2 complexed with ligand peptides for SH3 and SH2 domains
Yuzawa, S., Ogura, K., Hatanaka, H., Miura, K., Inagaki, F.
J. Biomol. NMR (2003), 27, 185-186, PubMed 12913417

Show more 1 citaion

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , DOI 10.1007/bf00197809 , Abstract

Hide non-primary 1 citaion

Related entities 1. growth factor receptor-bound protein 2, : 2 : 24 entities Detail

Related entities 3. Sos peptide, : 1 entities Detail

Experiments performed 7 experiments Detail

1 1H-15N HSQC