BMRB: 5708

Solution structure of the N-terminal membrane anchor of E. coli enzyme IIA(Glucose)

Authors

Wang, G., Keifer, P.A., Peterkofsky, A.

Assembly

PTS system, glucose-specific IIA component (E.C.2.7.1.69)

Entity

1. PTS system, glucose-specific IIA component (E.C.2.7.1.69) (polymer, Thiol state: not present), 15 monomers, 1692.950 Da Detail

GLFDKLKSLV SDDKK

Formula weight

1692.95 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

Simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 85.7 %, Completeness (bb): 96.8 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	85.7 % (84 of 98)	85.7 % (84 of 98)
Backbone	96.8 % (30 of 31)	96.8 % (30 of 31)
Sidechain	80.6 % (54 of 67)	80.6 % (54 of 67)
Aromatic	100.0 % (5 of 5)	100.0 % (5 of 5)
Methyl	100.0 % (8 of 8)	100.0 % (8 of 8)

1. PTS

GLFDKLKSLV SDDKK

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5.4

#	Name	Isotope labeling	Type	Concentration
1	PTS			5 mM
2	H2O			90 %
3	D2O			10 %
4	SDS			100 mM

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Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 82.7 %, Completeness (bb): 91.9 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	82.7 % (162 of 196)	82.7 % (162 of 196)
Backbone	91.9 % (57 of 62)	91.9 % (57 of 62)
Sidechain	78.4 % (105 of 134)	78.4 % (105 of 134)
Aromatic	100.0 % (10 of 10)	100.0 % (10 of 10)
Methyl	93.8 % (15 of 16)	93.8 % (15 of 16)

1. PTS

GLFDKLKSLV SDDKK

Show sample condition

Sample

Pressure 1 atm, Temperature 298 K, pH 5.4

#	Name	Isotope labeling	Type	Concentration
5	PTS			5 mM
6	DHPG			50 mM

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Protein Blocks Logo

Calculated from 20 models in PDB: 1O53, Strand ID: A Detail

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Release date

2003-05-21

Citation

Solution structure of the N-terminal amphitropic domain of Escherichia coli glucose-specific enzyme IIA in membrane-mimetic micelles
Wang, G., Keifer, P.A., Peterkofsky, A.
Protein Sci. (2003), 12, 1087-1096, PubMed 12717030 , DOI 10.1110/ps.0301503 , Abstract

Related entities 1. PTS system, glucose-specific IIA component (E.C.2.7.1.69), : 1 : 13 : 3 entities Detail

Experiments performed 3 experiments Detail