BMRB: 5713

Influence of pH on NMR structure and stability of the human prion protein globular domain

Authors

Calzolai, L., Zahn, R.

Assembly

human prion protein

Entity

1. human prion protein (polymer, Thiol state: all disulfide bound), 112 monomers, 13145.46 Da Detail

GSVVGGLGGY MLGSAMSRPI IHFGSDYEDR YYRENMHRYP NQVYYRPMDE YSNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCITQY ERESQAYYQR GS

Formula weight

13145.46 Da

Entity Connection

disulfide 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS61:SG	1:CYS96:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.5 %, Completeness (bb): 82.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	87.5 % (1144 of 1308)	97.2 % (663 of 682)	72.4 % (364 of 503)	95.1 % (117 of 123)
Backbone	82.0 % (546 of 666)	97.0 % (223 of 230)	67.3 % (220 of 327)	94.5 % (103 of 109)
Sidechain	93.7 % (698 of 745)	97.3 % (440 of 452)	87.5 % (244 of 279)	100.0 % (14 of 14)
Aromatic	71.6 % (96 of 134)	91.0 % (61 of 67)	52.2 % (35 of 67)
Methyl	100.0 % (86 of 86)	100.0 % (43 of 43)	100.0 % (43 of 43)

1. human prion protein

GSVVGGLGGY MLGSAMSRPI IHFGSDYEDR YYRENMHRYP NQVYYRPMDE YSNQNNFVHD CVNITIKQHT VTTTTKGENF TETDVKMMER VVEQMCITQY ERESQAYYQR GS

Show sample condition

Sample

Temperature 298 (±1) K, pH 7.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	human prion protein			0.8 ~ 1.3 mM

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LACS Plot; CA

Referencing offset: 0.38 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.38 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

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Protein Blocks Logo

Calculated from 1 models in PDB: 1HJM, Strand ID: A Detail

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Release date

2003-03-02

Citation

Influence of pH on NMR structure and stability of the human prion protein globular domain
Calzolai, L., Zahn, R.
J. Biol. Chem. (2003), 278, 35592-35596, PubMed 12826672 , DOI 10.1074/jbc.M303005200 , Abstract

Related entities 1. human prion protein, : 1 : 2 : 28 : 2 : 129 entities Detail

Interaction partners 1. human prion protein, : 70 interactors Detail

Experiments performed 1 experiments Detail