Instrument
Model Varian Inova (500 MHz)
Sample #1
Temperature 298 (±0.1) K, pH 7.0 (±0.1), Details A titration of the sample with the paramagnetic Ni(II) was performed. The
Ni(II) concentration was increased from 0.0mM to 1.0mM in steps of 0.1mM.
| # | Name | Isotope labeling | Type | Concentration |
|---|
| 1 | HHP-tagged E. coli Thioredoxin | [U-15N] | | 1.0 mM |
|---|
Sample #2
Temperature 298 (±0.1) K, pH 7.0 (±0.1)
| # | Name | Isotope labeling | Type | Concentration |
|---|
| 2 | HHP-tagged E. coli Thioredoxin | [U-15N] | | 1.0 mM |
|---|
| 3 | Nickel ion | | | 0.1 mM |
|---|
Sample #3
Temperature 298 (±0.1) K, pH 7.0 (±0.1)
| # | Name | Isotope labeling | Type | Concentration |
|---|
| 4 | HHP-tagged E. coli Thioredoxin | [U-15N] | | 1.0 mM |
|---|
| 5 | Nickel ion | | | 0.2 mM |
|---|
Sample #4
Temperature 298 (±0.1) K, pH 7.0 (±0.1)
| # | Name | Isotope labeling | Type | Concentration |
|---|
| 6 | HHP-tagged E. coli Thioredoxin | [U-15N] | | 1.0 mM |
|---|
| 7 | Nickel ion | | | 0.3 mM |
|---|
Sample #5
Temperature 298 (±0.1) K, pH 7.0 (±0.1)
| # | Name | Isotope labeling | Type | Concentration |
|---|
| 8 | HHP-tagged E. coli Thioredoxin | [U-15N] | | 1.0 mM |
|---|
| 9 | Nickel ion | | | 0.4 mM |
|---|
Sample #6
Temperature 298 (±0.1) K, pH 7.0 (±0.1)
| # | Name | Isotope labeling | Type | Concentration |
|---|
| 10 | HHP-tagged E. coli Thioredoxin | [U-15N] | | 1.0 mM |
|---|
| 11 | Nickel ion | | | 0.5 mM |
|---|
Sample #7
Temperature 298 (±0.1) K, pH 7.0 (±0.1)
| # | Name | Isotope labeling | Type | Concentration |
|---|
| 12 | HHP-tagged E. coli Thioredoxin | [U-15N] | | 1.0 mM |
|---|
| 13 | Nickel ion | | | 0.6 mM |
|---|
Sample #8
Temperature 298 (±0.1) K, pH 7.0 (±0.1)
| # | Name | Isotope labeling | Type | Concentration |
|---|
| 14 | HHP-tagged E. coli Thioredoxin | [U-15N] | | 1.0 mM |
|---|
| 15 | Nickel ion | | | 0.7 mM |
|---|
Sample #9
Temperature 298 (±0.1) K, pH 7.0 (±0.1)
| # | Name | Isotope labeling | Type | Concentration |
|---|
| 16 | HHP-tagged E. coli Thioredoxin | [U-15N] | | 1.0 mM |
|---|
| 17 | Nickel ion | | | 0.8 mM |
|---|
Sample #10
Temperature 298 (±0.1) K, pH 7.0 (±0.1)
| # | Name | Isotope labeling | Type | Concentration |
|---|
| 18 | HHP-tagged E. coli Thioredoxin | [U-15N] | | 1.0 mM |
|---|
| 19 | Nickel ion | | | 0.9 mM |
|---|
Sample #11
Temperature 298 (±0.1) K, pH 7.0 (±0.1)
| # | Name | Isotope labeling | Type | Concentration |
|---|
| 20 | HHP-tagged E. coli Thioredoxin | [U-15N] | | 1.0 mM |
|---|
| 21 | Nickel ion | | | 1.0 mM |
|---|
