BMRB: 5719

Resonance assignment and secondary structure of an N-terminal fragment of the human La protein

Authors

Alfano, C., Babon, J., Kelly, G., Curry, S., Conte, M.R.

Assembly

La protein

Entity

1. La protein (polymer, Thiol state: not present), 113 monomers, 13424.17 Da Detail

MGHHHHHHIE GRWILKNDVK NRSVYIKGFP TDATLDDIKE WLEDKGQVLN IQMRRTLHKA FKGSIFVVFD SIESAKKFVE TPGQKYKETD LLILFKDDYF AKKNEERKQN KVE

Formula weight

13424.17 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.7 %, Completeness: 82.2 %, Completeness (bb): 90.7 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	82.2 % (1167 of 1419)	80.1 % (598 of 747)	85.1 % (468 of 550)	82.8 % (101 of 122)
Backbone	90.7 % (611 of 674)	90.4 % (208 of 230)	91.3 % (304 of 333)	89.2 % (99 of 111)
Sidechain	76.6 % (653 of 852)	75.4 % (390 of 517)	80.6 % (261 of 324)	18.2 % (2 of 11)
Aromatic	67.1 % (98 of 146)	67.1 % (49 of 73)	66.2 % (47 of 71)	100.0 % (2 of 2)
Methyl	100.0 % (110 of 110)	100.0 % (55 of 55)	100.0 % (55 of 55)

1. La protein

MGHHHHHHIE GRWILKNDVK NRSVYIKGFP TDATLDDIKE WLEDKGQVLN IQMRRTLHKA FKGSIFVVFD SIESAKKFVE TPGQKYKETD LLILFKDDYF AKKNEERKQN KVE

Show sample condition

Sample

Temperature 293 (±0.5) K, pH 6.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	La protein			0.0 ~ 0.0 mM

Hide sample codition

LACS Plot; CA

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CB

Referencing offset: 0.09 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; HA

Referencing offset: -0.05 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

LACS Plot; CO

Referencing offset: 0.12 ppm, Outliers: 2 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Hide chemical shift referencing parameters

Protein Blocks Logo

Calculated from 20 models in PDB: 1S79, Strand ID: A Detail

---

Release date

2003-07-16

Citation

Resonance assignment and secondary structure of an N-terminal fragment of the human La protein
Alfano, C., Babon, J., Kelly, G., Curry, S., Conte, M.R.
J. Biomol. NMR (2003), 27, 93-94, PubMed 12878847 , DOI: , Abstract

Related entities 1. La protein, : 1 : 2 : 29 entities Detail

Interaction partners 1. La protein, : 83 interactors Detail

Experiments performed 1 experiments Detail