BMRB: 5720

15N Relaxation Data of Escherichia coli Adenylate Kinase in Ligand-Free Form Obtained at Magnetic Fields of 14.10 and 18.79 T

Authors

Shapiro, Y.E., Kahana, E., Tugarinov, V., Liang, Z., Freed, J.H., Meirovitch, E.

Assembly

Adenylate Kinase from Escherichia coli

Entity

1. Adenylate Kinase from Escherichia coli (polymer, Thiol state: all free), 214 monomers, 23585.72 Da Detail

MRIILLGAPG AGKGTQAQFI MEKYGIPQIS TGDMLRAAVK SGSELGKQAK DIMDAGKLVT DELVIALVKE RIAQEDCRNG FLLDGFPRTI PQADAMKEAG INVDYVLEFD VPDELIVDRI VGRRVHAPSG RVYHVKFNPP KVEGKDDVTG EELTTRKDDQ EETVRKRLVE YHQMTAPLIG YYSKEAEAGN TKYAKVDGTK PVAEVRADLE KILG

Formula weight

23585.72 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts, heteronucl_T1_relaxation, heteronucl_T2_relaxation, heteronucl_NOEs

Chem. Shift Complete

Sequence coverage: 89.3 %, Completeness: 27.7 %, Completeness (bb): 60.7 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	27.7 % (415 of 1498)	17.5 % (224 of 1282)	88.4 % (191 of 216)
Backbone	60.7 % (390 of 642)	45.4 % (199 of 438)	93.6 % (191 of 204)
Sidechain	2.8 % (24 of 856)	2.8 % (24 of 844)	0.0 % (0 of 12)
Aromatic	0.0 % (0 of 59)	0.0 % (0 of 59)
Methyl	6.3 % (8 of 128)	6.3 % (8 of 128)

1. Adenylate Kinase from Escherichia coli

MRIILLGAPG AGKGTQAQFI MEKYGIPQIS TGDMLRAAVK SGSELGKQAK DIMDAGKLVT DELVIALVKE RIAQEDCRNG FLLDGFPRTI PQADAMKEAG INVDYVLEFD VPDELIVDRI VGRRVHAPSG RVYHVKFNPP KVEGKDDVTG EELTTRKDDQ EETVRKRLVE YHQMTAPLIG YYSKEAEAGN TKYAKVDGTK PVAEVRADLE KILG

Show sample condition

Sample

Temperature 303 (±0.05) K, pH 6.8 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Adenylate Kinase from Escherichia coli	[U-15N]		1.75 mM

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Protein Blocks Logo

Calculated from 1 models in PDB: 1AKE, Strand ID: A, B Detail

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Heteronucl. T₁

382 T₁ values in 2 lists
Coherence Sz, Field strength (¹H) 600 MHz, 800 MHz, Temperature 303 (±0.05) K, pH 6.8 (±0.1) Detail

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Temperature 303 (±0.05) K, pH 6.8 (±0.1)

Heteronucl. T₂

382 T₂ values in 2 lists
Coherence S(+,-), Field strength (¹H) 600 MHz, 800 MHz, Temperature 303 (±0.05) K, pH 6.8 (±0.1) Detail

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Temperature 303 (±0.05) K, pH 6.8 (±0.1)

Heteronucl. NOE

381 NOE values in 2 lists
Value type na, Field strength (¹H) 600 MHz, 800 MHz, Temperature 303 (±0.05) K, pH 6.8 (±0.1) Detail

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Temperature 303 (±0.05) K, pH 6.8 (±0.1)

Heteronucl. T₁/T₂

382 T₁/T₂ values in 2 lists
Field strength (¹H) 600 MHz, 800 MHz, Temperature 303 (±0.05) K, pH 6.8 (±0.1) Detail

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Temperature 303 (±0.05) K, pH 6.8 (±0.1)

Release date

2003-04-30

Citation

Domain flexibility in ligand-free and inhibitor-bound Escherichia coli adenylate kinase based on a mode-coupling analysis of 15N spin relaxation
Shapiro, Y.E., Kahana, E., Tugarinov, V., Liang, Z., Freed, J.H., Meirovitch, E.
Biochemistry (2002), 41, 6271-6281, PubMed 12009888 , DOI: , Abstract

Related entities 1. Adenylate Kinase from Escherichia coli, : 1 : 10 : 4 : 14 : 269 entities Detail

Interaction partners 1. Adenylate Kinase from Escherichia coli, : 11 interactors Detail

Experiments performed 4 experiments Detail