Backbone 1NH, 13C and 15N Chemical shift assignments for the ternary DHFR:FOLATE:DHNADPH complex
MISLIAALAV DRVIGMENAM PWNLPADLAW FKRNTLDKPV IMGRHTWESI GRPLPGRKNI ILSSQPGTDD RVTWVKSVDE AIAACGDVPE IMVIGGGRVY EQFLPKAQKL YLTHIDAEVE GDTHFPDYEP DDWESVFSEF HDADAQNSHS YCFEILERR
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 75.9 % (1401 of 1845) | 78.7 % (752 of 955) | 68.6 % (499 of 727) | 92.0 % (150 of 163) |
Backbone | 84.2 % (786 of 934) | 95.9 % (305 of 318) | 71.9 % (336 of 467) | 97.3 % (145 of 149) |
Sidechain | 71.8 % (761 of 1060) | 70.2 % (447 of 637) | 75.6 % (309 of 409) | 35.7 % (5 of 14) |
Aromatic | 16.9 % (29 of 172) | 17.4 % (15 of 86) | 11.1 % (9 of 81) | 100.0 % (5 of 5) |
Methyl | 97.7 % (170 of 174) | 98.9 % (86 of 87) | 96.6 % (84 of 87) |
1. dihydrofolate reducatse
MISLIAALAV DRVIGMENAM PWNLPADLAW FKRNTLDKPV IMGRHTWESI GRPLPGRKNI ILSSQPGTDD RVTWVKSVDE AIAACGDVPE IMVIGGGRVY EQFLPKAQKL YLTHIDAEVE GDTHFPDYEP DDWESVFSEF HDADAQNSHS YCFEILERRTemperature 306.4 (±0.1) K
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | dihydrofolate reducatse | [U-13C; U-15N] | 1.2 mM | |
2 | FOLIC ACID | 20 mM | ||
3 | 5,6-dihydro nicotinamide adenine dinucleotide phosphate | 20 mM | ||
4 | potassium chloride | 100 mM | ||
5 | EDTA | 1 mM | ||
6 | DTT | 1 mM | ||
7 | potassium phosphate | 50 mM |