BMRB: 5745

NMR Structure of bovine cytochrome b5

Authors

Muskett, F.W., Whitford, D.

Assembly

bovine microsomal cytochrome b5

Entity

1. Cytochrome b5 (polymer, Thiol state: not present), 104 monomers, 11794.81 Da Detail

AEESSKAVKY YTLEEIQKHN NSKSTWLILH YKVYDLTKFL EEHPGGEEVL REQAGGDATE NFEDVGHSTD ARELSKTFII GELHPDDRSK ITKPSESIIT TIDS

2. HEM (non-polymer), 616.487 Da

Total weight

12411.297 Da

Max. entity weight

11794.81 Da

Entity Connection

covalent 1 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	covalent	sing	1:HIS67:HE2	2:HEM1:FE

Source organism

Bos taurus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 81.7 %, Completeness: 69.7 %, Completeness (bb): 76.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	69.7 % (839 of 1203)	71.8 % (448 of 624)	65.9 % (311 of 472)	74.8 % (80 of 107)
Backbone	76.4 % (472 of 618)	76.8 % (162 of 211)	75.2 % (230 of 306)	79.2 % (80 of 101)
Sidechain	65.3 % (446 of 683)	69.2 % (286 of 413)	60.6 % (160 of 264)	0.0 % (0 of 6)
Aromatic	12.8 % (12 of 94)	25.5 % (12 of 47)	0.0 % (0 of 46)	0.0 % (0 of 1)
Methyl	76.9 % (83 of 108)	77.8 % (42 of 54)	75.9 % (41 of 54)

1. Cytochrome b5

AEESSKAVKY YTLEEIQKHN NSKSTWLILH YKVYDLTKFL EEHPGGEEVL REQAGGDATE NFEDVGHSTD ARELSKTFII GELHPDDRSK ITKPSESIIT TIDS

Show sample condition

Sample

Temperature 300 (±0.1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Cytochrome b5	[U-13C; U-15N]		1.0 ~ 2.0 mM
2	Phosphate			20 mM

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Protein Blocks Logo

Calculated from 42 models in PDB: 1HKO, Strand ID: A Detail

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Release date

2003-12-18

Citation

The solution structure of bovine ferricytochrome b5 determined using heteronuclear NMR methods
Muskett, F.W., Whitford, D.
J. Mol. Biol. (1996), 258, 172-189, PubMed 8613986 , DOI 10.1006/jmbi.1996.0241 , Abstract

Related entities 1. Cytochrome b5, : 1 : 1 : 1 : 120 entities Detail

Interaction partners 1. Cytochrome b5, : 4 interactors Detail

Experiments performed 9 experiments Detail