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BMRB: 5759

2A1M

A model for effector activity in a highly specific biological electron transfer complex: The cytochrome P450cam-putidaredoxin couple

Authors

Pochapsky, S.SONDEJ., Pochapsky, T.C., Wei, J.W.

Assembly

cytochrome P450cam

Entity

1. cytochrome P450cam (polymer, Thiol state: all free), 414 monomers, 46505.41 Da Detail

TTETIQSNAN LAPLPPHVPE HLVFDFDMYN PSNLSAGVQE AWAVLQESNV PDLVWTRCNG GHWIATRGQL IREAYEDYRH FSSECPFIPR EAGEAYDFIP TSMDPPEQRQ FRALANQVVG MPVVDKLENR IQELACSLIE SLRPQGQCNF TEDYAEPFPI RIFMLLAGLP EEDIPHLKYL TDQMTRPDGS MTFAEAKEAL YDYLIPIIEQ RRQKPGTDAI SIVANGQVNG RPITSDEAKR MCGLLLVGGL DTVVNFLSFS MEFLAKSPEH RQELIERPER IPAACEELLR RFSLVADGRI LTSDYEFHGV QLKKGDQILL PQMLSGLDER ENAAPMHVDF SRQKVSHTTF GHGSHLCLGQ HLARREIIVT LKEWLTRIPD FSIAPGAQIQ HKSGIVSGVQ ALPLVWDPAT TKAV

2. HEM (non-polymer), 616.487 Da

Total weight

47121.9 Da

Max. entity weight

46505.41 Da

Source organism

Pseudomonas putida

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 49.3 %, Completeness: 21.2 %, Completeness (bb): 35.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	21.2 % (1022 of 4830)	13.2 % (333 of 2515)	27.5 % (520 of 1890)	39.8 % (169 of 425)
Backbone	35.1 % (851 of 2424)	24.7 % (203 of 823)	39.4 % (480 of 1217)	43.8 % (168 of 384)
Sidechain	11.1 % (309 of 2795)	7.7 % (131 of 1692)	16.7 % (177 of 1062)	2.4 % (1 of 41)
Aromatic	2.2 % (8 of 364)	2.2 % (4 of 182)	2.3 % (4 of 177)	0.0 % (0 of 5)
Methyl	10.6 % (50 of 472)	9.3 % (22 of 236)	11.9 % (28 of 236)

1. cytochrome P450cam

Show sample condition

Sample

Temperature 308 (±0.1) K, pH 7.4 (±0.2)

#	Name	Isotope labeling	Concentration
1	cytochrome P450cam	[U-15N; U-13C, U-85% 2H]	0.2 ~ 0.6 mM
2	TrisCl	[U-2H]	50 mM
3	KCl		150 mM
4	camphor	[U-2H]	1 mM
5	H2O		90 %
6	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.47 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.47 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.33 ppm, Outliers: 1 Detail

Release date

2004-04-06

Citation 1

A model for effector activity in a highly specific biological electron transfer complex: the cytochrome P450(cam)-putidaredoxin couple
Pochapsky, S.SONDEJ., Pochapsky, T.C., Wei, J.W.
Biochemistry (2003), 42, 5649-5656, PubMed 12741821 , DOI 10.1021/bi034263s , Abstract

Show more 1 citaion

Citation 2

The dimerization of Pseudomonas putida cytochrome P450cam: practical consequences and engineering of a monomeric enzyme
Nickerson, D.P., Wong, L.L.
Protein Eng. (1997), 10, 1357-1361, PubMed 9542996 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. cytochrome P450cam, : 1 : 23 : 5 : 60 : 52 entities Detail

Interaction partners 1. cytochrome P450cam, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 14 experiments Detail

1 Experiments were run with 16 scans per t1 point, 512 (1H) x 32 (13C) x 32 (15N)