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Found 1 Document (1.302 seconds)

BMRB: 5761

1GYU

1H, 13C and 15N Chemical Shift Assignments for Gamma-Adaptin Ear Domain

Authors

Denisov, A.YU., Gehring, K.

Assembly

gamma-adaptin ear domain

Entity

1. gamma-adaptin ear domain (polymer, Thiol state: not present), 119 monomers, 13274.98 Da Detail

IPSITAYSKN GLKIEFTFER SNTNPSVTVI TIQASNSTEL DMTDFVFQAA VPKTFQLQLL SPSSSVVPAF NTGTITQVIK VLNPQKQQLR MRIKLTYNHK GSAMQDLAEV NNFPPQSWQ

Formula weight

13274.98 Da

Source organism

Mus musculus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 96.6 %, Completeness: 56.3 %, Completeness (bb): 89.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	56.3 % (798 of 1418)	47.6 % (351 of 737)	62.1 % (341 of 549)	80.3 % (106 of 132)
Backbone	89.7 % (626 of 698)	82.0 % (191 of 233)	93.5 % (331 of 354)	93.7 % (104 of 111)
Sidechain	34.0 % (284 of 836)	31.7 % (160 of 504)	39.2 % (122 of 311)	9.5 % (2 of 21)
Aromatic	6.9 % (7 of 102)	11.8 % (6 of 51)	0.0 % (0 of 50)	100.0 % (1 of 1)
Methyl	31.7 % (45 of 142)	49.3 % (35 of 71)	14.1 % (10 of 71)

1. gamma-adaptin ear domain

IPSITAYSKN GLKIEFTFER SNTNPSVTVI TIQASNSTEL DMTDFVFQAA VPKTFQLQLL SPSSSVVPAF NTGTITQVIK VLNPQKQQLR MRIKLTYNHK GSAMQDLAEV NNFPPQSWQ

Show sample condition

Sample #1

Temperature 303 (±0.5) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	gamma-adaptin ear domain	[U-99% 13C; U-99% 15N]	1.5 mM
2	sodium phosphate buffer		50 mM
3	NaCl		150 mM

Sample #2

Temperature 303 (±0.5) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
4	gamma-adaptin ear domain	[U-99% 15N]	1.5 mM
5	sodium phosphate buffer		50 mM
6	NaCl		150 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 1.38 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 1.38 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.28 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.14 ppm, Outliers: 4 Detail

Release date

2003-12-18

Citation

Characterization of a gamma-adaptin ear-binding motif in enthoprotin
Wasiak, S., Denisov, A.YU., Han, Z., Leventis, P.A., Heuvel, E., Boulianne, G.L., Kay, B.K., Gehring, K., McPherson, P.S.
FEBS Lett. (2003), 555, 437-442, PubMed 14675752 , DOI: , Abstract

Related entities 1. gamma-adaptin ear domain, : 1 : 3 : 4 : 2 : 53 entities Detail

Interaction partners 1. gamma-adaptin ear domain, : 7 interactors Detail

More details for 7 interactors identified by 5 citations

Experiments performed 6 experiments Detail

1 3D HNCACB

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	gamma-adaptin ear domain	[U-99% 13C; U-99% 15N]	1.5 mM
2	sodium phosphate buffer		50 mM
3	NaCl		150 mM

Sample #2

Temperature 303 (±0.5) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
4	gamma-adaptin ear domain	[U-99% 15N]	1.5 mM
5	sodium phosphate buffer		50 mM
6	NaCl		150 mM

2 3D CBCA(CO)HN

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	gamma-adaptin ear domain	[U-99% 13C; U-99% 15N]	1.5 mM
2	sodium phosphate buffer		50 mM
3	NaCl		150 mM

Sample #2

Temperature 303 (±0.5) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
4	gamma-adaptin ear domain	[U-99% 15N]	1.5 mM
5	sodium phosphate buffer		50 mM
6	NaCl		150 mM

3 3D HNCO

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	gamma-adaptin ear domain	[U-99% 13C; U-99% 15N]	1.5 mM
2	sodium phosphate buffer		50 mM
3	NaCl		150 mM

Sample #2

Temperature 303 (±0.5) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
4	gamma-adaptin ear domain	[U-99% 15N]	1.5 mM
5	sodium phosphate buffer		50 mM
6	NaCl		150 mM

4 3D 15N-HMQC-NOESY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	gamma-adaptin ear domain	[U-99% 13C; U-99% 15N]	1.5 mM
2	sodium phosphate buffer		50 mM
3	NaCl		150 mM

Sample #2

Temperature 303 (±0.5) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
4	gamma-adaptin ear domain	[U-99% 15N]	1.5 mM
5	sodium phosphate buffer		50 mM
6	NaCl		150 mM

5 3D 15N-HMQC-TOCSY

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	gamma-adaptin ear domain	[U-99% 13C; U-99% 15N]	1.5 mM
2	sodium phosphate buffer		50 mM
3	NaCl		150 mM

Sample #2

Temperature 303 (±0.5) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
4	gamma-adaptin ear domain	[U-99% 15N]	1.5 mM
5	sodium phosphate buffer		50 mM
6	NaCl		150 mM

6 2D 15N-HSQC

Instrument

Model Bruker DRX (600 MHz)

Sample #1

Temperature 303 (±0.5) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
1	gamma-adaptin ear domain	[U-99% 13C; U-99% 15N]	1.5 mM
2	sodium phosphate buffer		50 mM
3	NaCl		150 mM

Sample #2

Temperature 303 (±0.5) K, pH 7.2 (±0.1)

#	Name	Isotope labeling	Concentration
4	gamma-adaptin ear domain	[U-99% 15N]	1.5 mM
5	sodium phosphate buffer		50 mM
6	NaCl		150 mM

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5761_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5761_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	828		96.6 (chain: 1, length: 119)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 119, Sequence coverage: 96.6%
- Total number of assignments
  - ¹³C chemical shifts: 330
  - ¹⁵N chemical shifts: 105
  - ¹H chemical shifts: 393
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	549	330	60.1
¹⁵N chemical shifts	135	105	77.8
¹H chemical shifts	737	327	44.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	238	218	91.6
¹⁵N chemical shifts	111	104	93.7
¹H chemical shifts	233	190	81.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	311	112	36.0
¹⁵N chemical shifts	24	1	4.2
¹H chemical shifts	504	137	27.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	74	7	9.5
¹H chemical shifts	74	33	44.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	50	0	0.0
¹⁵N chemical shifts	1	1	100.0
¹H chemical shifts	51	1	2.0

Keywords Gamma-adaptin, NMR assignments, enthoprotin

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Found 1 Document (1.302 seconds)

BMRB: 5761

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. gamma-adaptin ear domain, : 1 : 3 : 4 : 2 : 53 entities Detail

Interaction partners 1. gamma-adaptin ear domain, : 7 interactors Detail

Experiments performed 6 experiments Detail

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Chemical shift validation 3 contents Detail