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BMRB: 5772

1KNL

Backbone 1H, 13C and 15N chemical shifts of CBM13 from Streptomyces lividans

Authors

Schaerpf, M., McIntosh, L.P.

Assembly

SlxA CBM13

Entity

1. SlxA CBM13 (polymer, Thiol state: all free), 142 monomers, 15035.14 Da Detail

HHHHHHIEGR ASEPPADGGQ IKGVGSGRCL DVPDASTSDG TQLQLWDCHS GTNQQWAATD AGELRVYGDK CLDAAGTSNG SKVQIYSCWG GDNQKWRLNS DGSVVGVQSG LCLDAVGNGT ANGTLIQLYT CSNGSNQRWT RT

Formula weight

15035.14 Da

Source organism

Streptomyces lividans

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.1 %, Completeness: 74.3 %, Completeness (bb): 92.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	74.3 % (1113 of 1497)	65.4 % (502 of 768)	81.5 % (462 of 567)	92.0 % (149 of 162)
Backbone	92.1 % (779 of 846)	91.1 % (275 of 302)	92.1 % (373 of 405)	94.2 % (131 of 139)
Sidechain	57.0 % (440 of 772)	47.2 % (220 of 466)	71.4 % (202 of 283)	78.3 % (18 of 23)
Aromatic	0.9 % (1 of 112)	0.0 % (0 of 56)	0.0 % (0 of 51)	20.0 % (1 of 5)
Methyl	64.8 % (83 of 128)	54.7 % (35 of 64)	75.0 % (48 of 64)

1. family 13 carbohydrate binding modul

HHHHHHIEGR ASEPPADGGQ IKGVGSGRCL DVPDASTSDG TQLQLWDCHS GTNQQWAATD AGELRVYGDK CLDAAGTSNG SKVQIYSCWG GDNQKWRLNS DGSVVGVQSG LCLDAVGNGT ANGTLIQLYT CSNGSNQRWT RT

Show sample condition

Sample

Temperature 303 (±2) K, pH 6.1 (±0.1)

#	Name	Isotope labeling	Concentration
1	family 13 carbohydrate binding modul	[U-13C; U-15N]	1 mM
2	potassium phosphate		50 mM
3	potassium chloride		50 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.96 ppm, Outliers: 2 Detail

Release date

2003-05-21

Citation

Site-specific characterization of the association of xylooligosaccharides with the CBM13 lectin-like xylan binding domain from Streptomyces lividans xylanase 10A by NMR spectroscopy
Scharpf, M., Connelly, G.P., Lee, G.M., Boraston, A.B., Warren, R.A., McIntosh, L.P.
Biochemistry (2002), 41, 4255-4263, PubMed 11914071 , DOI: , Abstract

Related entities 1. SlxA CBM13, : 1 : 2 : 124 entities Detail

Experiments performed 9 experiments Detail

1 1H-15N-HSQC