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Found 1 Document (2.144 seconds)

BMRB: 5782

7R7Y

1H chemical shift assignment for the truncated form of the human beta2-microglobulin lacking of the first 3 residues

Authors

Viglino, P., Corazza, A., Pettirossi, F., Verdone, G., Esposito, G.

Assembly

DN3beta2-microglobulin

Entity

1. DN3beta2-microglobulin (polymer, Thiol state: all disulfide bound), 97 monomers, 11464.74 Da Detail

MTPKIQVYSR HPAENGKSNF LNCYVSGFHP SDIEVDLLKN GERIEKVEHS DLSFSKDWSF YLLYYTEFTP TEKDEYACRV NHVTLSQPKI VKWDRDM

Formula weight

11464.74 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS23:SG	1:CYS78:SG

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 100.0 %, Completeness: 86.3 %, Completeness (bb): 96.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	86.3 % (531 of 615)	86.3 % (531 of 615)
Backbone	96.4 % (185 of 192)	96.4 % (185 of 192)
Sidechain	81.8 % (346 of 423)	81.8 % (346 of 423)
Aromatic	66.7 % (46 of 69)	66.7 % (46 of 69)
Methyl	95.3 % (41 of 43)	95.3 % (41 of 43)

1. beta2-microglobulin

MTPKIQVYSR HPAENGKSNF LNCYVSGFHP SDIEVDLLKN GERIEKVEHS DLSFSKDWSF YLLYYTEFTP TEKDEYACRV NHVTLSQPKI VKWDRDM

Show sample condition

Sample #1

Temperature 310 (±0.1) K, pH 6.5 (±0.1)

#	Name	Concentration
1	beta2-microglobulin	0.86 mM
2	sodium phosphate	70 mM
3	sodium chloride	100 mM
4	H2O	100 %

Sample #2

Temperature 310 (±0.1) K, pH 6.5 (±0.1)

#	Name	Concentration
5	beta2-microglobulin	0.7 mM
6	sodium phosphate	70 mM
7	sodium chloride	100 mM
8	D2O	95 %
9	H2O	5 %

Chem. Shift Complete2

Sequence coverage: 40.2 %, Completeness: 55.2 %, Completeness (bb): 66.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	55.2 % (679 of 1230)	55.2 % (679 of 1230)
Backbone	66.4 % (255 of 384)	66.4 % (255 of 384)
Sidechain	50.1 % (424 of 846)	50.1 % (424 of 846)
Aromatic	37.0 % (51 of 138)	37.0 % (51 of 138)
Methyl	64.0 % (55 of 86)	64.0 % (55 of 86)

1. beta2-microglobulin

MTPKIQVYSR HPAENGKSNF LNCYVSGFHP SDIEVDLLKN GERIEKVEHS DLSFSKDWSF YLLYYTEFTP TEKDEYACRV NHVTLSQPKI VKWDRDM

Show sample condition

Sample #1

Temperature 310 (±0.1) K, pH 6.5 (±0.1)

#	Name	Concentration
1	beta2-microglobulin	0.86 mM
2	sodium phosphate	70 mM
3	sodium chloride	100 mM
4	H2O	100 %

Sample #2

Temperature 310 (±0.1) K, pH 6.5 (±0.1)

#	Name	Concentration
5	beta2-microglobulin	0.7 mM
6	sodium phosphate	70 mM
7	sodium chloride	100 mM
8	D2O	95 %
9	H2O	5 %

Release date

2003-04-23

Citation 1

Properties of some variants of human beta2-microglobulin and amyloidogenesis
Corazza, A., Pettirossi, F., Viglino, P., Verdone, G., Garcia, J., Dumy, P., Giorgetti, S., Mangione, P., Andreola, A., Stoppini, M., Bellotti, V., Esposito, G.
J. Biol. Chem. (2004), 279, 9176-9189, PubMed 14660575 , DOI 10.1074/jbc.M310779200 , Abstract

Show more 2 citaions

Citation 2

The solution structure of human beta2-microglobulin reveals the prodromes of its amyloid transition
Verdone, G., Corazza, A., Viglino, P., Pettirossi, F., Giorgetti, S., Mangione, P., Andreola, A., Stoppini, M., Bellotti, V., Esposito, G.
Protein Sci. (2002), 11, 487-499, PubMed 11847272 , DOI 10.1110/ps.29002 , Abstract

Citation 3

Removal of the N-terminal hexapeptide from human beta2-microglobulin facilitates protein aggregation and fibril formation
Esposito, G., Michelutti, R., Verdone, G., Viglino, P., Hernandez, H., Robinson, C.V., Amoresano, A., Dal Piaz, F., Monti, M., Pucci, P., Mangione, P., Stoppini, M., Merlini, G., Ferri, G., Bellotti, V.
Protein Sci. (2000), 9, 831-845, PubMed 10850793 , DOI 10.1110/ps.9.5.831 , Abstract

Hide non-primary 2 citaions

Entries sharing articles BMRB: 3 entries Detail

  BMRB: 5783 released on 2003-04-23
    Title 1H chemical shift assignments for a mutant of human beta2-microglobulin where ARG 3 is replaced by Ala
  BMRB: 5784 released on 2003-04-23
    Title 1H assignment of a human variant of beta2-microglobulin where His 31 was replaced by a Tyr
  BMRB: 5169 released on 2002-05-05
    Title NMR solution structure of the human beta2-microglobulin

Related entities 1. DN3beta2-microglobulin, : 1 : 103 : 183 entities Detail

Interaction partners 1. DN3beta2-microglobulin, : 121 interactors Detail

More details for 121 interactors identified by 43 citations

Experiments performed 3 experiments Detail

Chemical shift validation 4 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5782_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	True (see coordinates for details)
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:23:CYS:SG	1:78:CYS:SG	unknown	unknown	n/a

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5782_3.str

#	Content subtype	Saveframe	Status	# of rows	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	612	100.0 (chain: 1, length: 97)
2	Assigned chemical shifts	shift_set_2	Warning	104	26.8 (chain: 1, length: 97)
1	Chemical shift references	chemical_shift_reference	OK	1	No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 97, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 612
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: shift_set_2
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 97, Sequence coverage: 26.8%
- Total number of assignments
  - ¹H chemical shifts: 104
- Completeness of assignments
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	615	530	86.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	192	185	96.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	423	345	81.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	45	41	91.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	69	46	66.7

Keywords amyloidosis, beta2-microglobulin

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Found 1 Document (2.144 seconds)

BMRB: 5782

Sample #1

Sample #2

Sample #1

Sample #2

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. DN3beta2-microglobulin, : 1 : 103 : 183 entities Detail

Interaction partners 1. DN3beta2-microglobulin, : 121 interactors Detail

Experiments performed 3 experiments Detail

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Instrument

Sample #1

Sample #2

Chemical shift validation 4 contents Detail