BMRB: 5801

Structural analysis of an EGF/TGF-alpha chimera with unique ErbB binding specificity

Authors

Wingens, M., Walma, T., van Ingen, H., Stortelers, C., van Leeuwen, J.E.M., van Zoelen, E.J.J., Vuister, G.W.

Assembly

T1E

Entity

1. T1E (polymer, Thiol state: all disulfide bound), 54 monomers, 6332.165 Da Detail

VVSHFNDCPL SHDGYCLHDG VCMYIEALDK YACNCVVGYI GERCQYRDLK WWEL

Formula weight

6332.165 Da

Entity Connection

disulfide 3 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS8:SG	1:CYS22:SG
2	disulfide	sing	1:CYS16:SG	1:CYS33:SG
3	disulfide	sing	1:CYS35:SG	1:CYS44:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry followed by brief molecular dynamics run in water.

Data set

assigned_chemical_shifts, coupling_constants, heteronucl_NOEs, heteronucl_T1_relaxation

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.4 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	93.4 % (354 of 379)	92.8 % (298 of 321)	96.6 % (56 of 58)
Backbone	100.0 % (164 of 164)	100.0 % (111 of 111)	100.0 % (53 of 53)
Sidechain	88.4 % (190 of 215)	89.0 % (187 of 210)	60.0 % (3 of 5)
Aromatic	71.1 % (32 of 45)	69.8 % (30 of 43)	100.0 % (2 of 2)
Methyl	92.3 % (24 of 26)	92.3 % (24 of 26)

1. T1E

VVSHFNDCPL SHDGYCLHDG VCMYIEALDK YACNCVVGYI GERCQYRDLK WWEL

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.3 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	T1E	[U-15N]		0.8 mM
2	K2HPO4/KH2PO4			50 mM

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Protein Blocks Logo

Calculated from 36 models in PDB: 1P9J, Strand ID: A Detail

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Heteronucl. T₁

52 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 600 MHz, Temperature 298 (±1) K, pH 6.3 (±0.2) Detail

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Temperature 298 (±1) K, pH 6.3 (±0.2)

Heteronucl. NOE

52 NOE values in 1 lists
Value type na, Field strength (¹H) 600 MHz, Temperature 298 (±1) K, pH 6.3 (±0.2) Detail

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Temperature 298 (±1) K, pH 6.3 (±0.2)

Coupling constant

51 J values in 1 lists
Temperature 298 (±1) K, pH 6.3 (±0.2) Detail

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Temperature 298 (±1) K, pH 6.3 (±0.2)

List #1 J_values_set_1

Release date

2003-10-12

Citation

Structural analysis of an epidermal growth factor/transforming growth factor-alpha chimera with unique ErbB binding specificity
Wingens, M., Walma, T., van Ingen, H., Stortelers, C., van Leeuwen, J.E.M., van Zoelen, E.J.J., Vuister, G.W.
J. Biol. Chem. (2003), 278, 39114-39123, PubMed 12869572 , DOI 10.1074/jbc.M305603200 , Abstract

Related entities 1. T1E, : 1 : 102 entities Detail

Interaction partners 1. T1E, : 7 interactors Detail

Experiments performed 5 experiments Detail